Effects of gonadotropin-releasing hormone receptor antagonists on ectopic human trophoblast cell invasion
department or school: Medicine, Faculty of
department or school: Obstetrics and Gynaecology, Department of
data provider: DSpace
degree campus: UBCV
creator: Abdellatif, Lobna
issued: 2019-08-21T16:43:16Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Ectopic pregnancy (EP) is a life-threatening condition responsible for 6.5% of Canadian mothers’ death between 1993-2004. EP occurs when an embryo implants outside the uterine endometrium, commonly in the Fallopian tube (98%). Tubal EP often requires surgical intervention as a life-saving measure. Early and precise diagnosis allow many women to choose methotrexate, the only available medical option, for treatment. Methotrexate is effective in 70% of patients; however, they may require multiple treatments, suffer moderate-to-severe side effects, or experience sub-optimal responses. The remaining 30% fail to respond to the treatment. Gonadotropin-releasing hormone (GnRH) has an inducing effect on intrauterine placental cell invasion and human chorionic gonadotropin hormone (hCG) production. GnRH receptor (GnRH-R) antagonists have been used safely to treat endometriosis, chronic pelvic pain, and in the artificial fertilization techniques. For the first time, we separated primary trophoblast cells from tubal EP placentas. We validated their trophoblastic origin using trophoblast-validating markers. The preliminary findings showed that primary EP trophoblast cells express GnRH and GnRH receptor. Treating these cells with clinical GnRH-R antagonist, cetrorelix, suppressed their viability. We used primary trophoblast cells from tubal pregnancies, immortalized HTR-8/SVneo cell line, and placental villous explants established from the first-trimester human intrauterine placenta in this study. We investigated the effect of clinical GnRH-R antagonists, cetrorelix and ganirelix, on the invasion of different trophoblast models. Finding a safe, effective, and more tolerant medical treatment for tubal pregnancy will significantly improve women’s health and reduce healthcare-related costs.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/71368
note: Effects of Gonadotropin-Releasing Hormone Receptor Antagonists on Ectopic Human Trophoblast Cell Invasion by Lobna Abdellatif MBBS, Minia University, Egypt, 2010 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (REPRODUCTIVE AND DEVELOPMENTAL SCIENCES) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2019 © Lobna Abdellatif, 2019 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis/dissertation entitled: Effect of Gonadotropin-releasing hormone receptor antagonists on ectopic human trophoblast cell invasion submitted by Lobna Abdellatif in partial fulfillment of the requirements for the degree of Master of Science in Reproductive and Developmental Science Examining Committee: Dr. Mohamed Bedaiwy Supervisor Dr. Alexander Beristain Supervisory Committee Member Dr. Christian Klausen Supervisory Committee Member Dr. Paul Yong Supervisory Committee Member Additional Supervisory Committee Members: Dr. Dan Rurak Additional Examiner iii Abstract Ectopic pregnancy (EP) is a life-threatening condition responsible for 6.5% of Canadian mothers’ death between 1993-2004. EP occurs when an embryo implants outside the uterine endometrium, commonly in the Fallopian tube (98%). Tubal EP often requires surgical intervention as a life-saving measure. Early and precise diagnosis allow many women to choose methotrexate, the only available medical option, for treatment. Methotrexate is effective in 70% of patients; however, they may require multiple treatments, suffer moderate-to-severe side effects, or experience sub-optimal responses. The remaining 30% fail to respond to the treatment. Gonadotropin-releasing hormone (GnRH) has an inducing effect on intrauterine placental cell invasion and human chorionic gonadotropin hormone (hCG) production. GnRH receptor (GnRH-R) antagonists have been u…
has type: Thesis/Dissertation
date issued: 2019-09
is shown at: 10.14288/1.0380536
language: eng
degree discipline: Reproductive and Developmental Sciences
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/71368