Identifying a native p-hydroxybenzoyl-CoA monolignol transferase gene in poplar
department or school: Forestry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: MacKay, Heather A.
issued: 2019-08-01T16:37:15Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Woody biomass utilisation for the production of pulp and paper, specialty cellulose and chemicals, and cellulosic biofuels requires significant investment of chemicals and energy. Lignin, a polyphenolic constituent of the secondary cell walls of vascular plants, is a significant impediment for wood processing. As such, delignification is essential for biomass utilisation, and typically relies on the addition of acid or alkali to promote fibre separation, and the recovery of the chemical fractions of the biomass. Increasing the degree of acylation (the process of adding an acyl group, or RCO– group, to a compound) of lignin and/or integrating unique bonds into the lignin polymer backbone can improve the processability of an otherwise chemically stable polymer. In Salix, Populus, and Palmae species, p-hydroxybenzoic acid (pHBA) acylates the monolignols. When these monolignol p-hydroxybenzoate conjugates are incorporated into lignin, pendant ester groups decorate the lignin polymer, and can be easily ‘clipped off’ by mild chemical treatment. Consequently, pHBA is of particular interest in the pursuit of creating designer lignins. p-HYDROXYBENZOYL-COA MONOLIGNOL TRANSFERASE (pHBMT) is the putative enzyme thought to be responsible for decorating monolignols with pHBA. The gene encoding pHBMT, however, remains elusive. In this study a putative pHBMT gene was identified through in vitro enzyme activity assays, and subsequently overexpressed in both A. thaliana and hybrid poplar under the control of the Cauliflower mosaic virus 35S promoter (CaMV35SP) and the Arabidopsis thaliana CINNAMATE-4-HYDROXYLASE promoter (AtC4HP). Although, overexpression of this putative pHBMT did not result in a significant increase in pHBA in the cell walls of A. thaliana, increases were observed in poplar. This result furthers our understandings of the mechanisms driving acylated monolignol biosynthesis, and identified a promising avenue in the pursuit and development of novel transgenic plant v…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/71174
note: Identifying a native p-hydroxybenzoyl-CoA monolignol transferase gene in poplar by Heather A. MacKay B.Sc., Queen’s University, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Forestry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) July 2019 © Heather A. MacKay, 2019 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Identifying a native p-hydroxybenzoyl-CoA monolignol transferase gene in poplar submitted by Heather A. MacKay in partial fulfillment of the requirements for the degree of Master of Science in Forestry Examining Committee: Shawn D. Mansfield Supervisor Lacey Samuels Supervisory Committee Member Simone D. Castellarin Supervisory Committee Member Harry Brumer Additional Examiner iii Abstract Woody biomass utilisation for the production of pulp and paper, specialty cellulose and chemicals, and cellulosic biofuels requires significant investment of chemicals and energy. Lignin, a polyphenolic constituent of the secondary cell walls of vascular plants, is a significant impediment for wood processing. As such, delignification is essential for biomass utilisation, and typically relies on the addition of acid or alkali to promote fibre separation, and the recovery of the chemical fractions of the biomass. Increasing the degree of acylation (the process of adding an acyl group, or RCO– group, to a compound) of lignin and/or integrating unique bonds into the lignin polymer backbone can improve the processability of an otherwise chemically stable polymer. In Salix, Populus, and Palmae species, p-hydroxybenzoic acid (pHBA) acylates the monolignols. When these monolignol p-hydroxybenzoate conjugates are incorporated into lignin, pendant ester groups decorate the lignin polymer, and can be easily ‘clipped off’ by mild chemical treatment. Consequently, …
has type: Thesis/Dissertation
date issued: 2019-09
is shown at: 10.14288/1.0380258
language: eng
degree discipline: Forestry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/71174