Adipose-derived stem cells improve tissue quality in a murine model of delayed wound healing
department or school: Medicine, Faculty of
department or school: Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Forbes, Diana
issued: 2019-05-09T13:51:03Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Wound repair and regeneration is a multidisciplinary field of research with considerable potential value to the management of deep and large cutaneous injuries such as ulcers caused by pressure, venous and arterial insufficiency, and diabetes. These injuries lack an appropriate tissue scaffold and pro-healing cells making them difficult to heal. An ideal therapy is one that would restore both the essential matrix and cellular components that are lacking in these wounds to expedite healing. In this study, we used a novel liquid dermal scaffold capable of gelation in vivo to show that it is biocompatible with adipose derived cells. Using a validated method of wound splinting in an impaired-healing murine model we showed that wounds treated with adipose derived stem cells and the liquid scaffold had improved epithelialization, angiogenesis, and collagen content. The addition of adipose derived cells showed significantly increased expressions of essential growth factors: vascular endothelial factor (VEGF) and hepatocyte growth factor (HGF) in vivo and that these cells are detectable in the neodermis after wound closure. This liquid dermal scaffold with cells can be considered as a feasible future strategy for the management of complex or deep wounds that are otherwise lacking the appropriate cellular matrix necessary for healing.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/70129
note: Adipose-derived stem cells improve tissue quality in a murine model of delayed wound healing by DIANA FORBES BSc (Honours), The University of British Columbia, 2010 MD, The University of British Columbia, 2014 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) May 2019 ©Diana Forbes, 2019 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Adipose-derived stem cells improve tissue quality in a murine model of delayed wound healing submitted by Diana Forbes in partial fulfillment of the requirements for the degree of Master of Science in Experimental Medicine Examining Committee: Dr. Aziz Ghahary Supervisor Dr. Reza Jalili Supervisory Committee Member Dr. Erin Brown Supervisory Committee Member Dr. Dirk Lange University Examiner iii Abstract Wound repair and regeneration is a multidisciplinary field of research with considerable potential value to the management of deep and large cutaneous injuries such as ulcers caused by pressure, venous and arterial insufficiency, and diabetes. These injuries lack an appropriate tissue scaffold and pro-healing cells making them difficult to heal. An ideal therapy is one that would restore both the essential matrix and cellular components that are lacking in these wounds to expedite healing. In this study, we used a novel liquid dermal scaffold capable of gelation in vivo to show that it is biocompatible with adipose derived cells. Using a validated method of wound splinting in an impaired-healing murine model we showed that wounds treated with adipose derived stem cells and the liquid scaffold had improved epithelialization, angiogenesis, and collagen content. The addition of adipose derived cells showed significantly increased expressions of essential…
has type: Thesis/Dissertation
date issued: 2019-09
is shown at: 10.14288/1.0378658
language: eng
degree discipline: Experimental Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/70129