Use of small molecule activity in upregulation of Class I MHC and change in tumour cytokine profile in metastatic murine epithelial carcinoma.
department or school: Science, Faculty of
department or school: Microbiology and Immunology, Department of
data provider: DSpace
degree campus: UBCV
creator: Dada, Sarah
issued: 2021-04-30T07:00:00Z
issued: 2019
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Cancer is a devastating disease that is responsible for the death of a quarter of Canadians. Cancer is the result of cells that gain genetic mutations or epigenetic changes that incur abnormal, uncontrolled proliferation. Oftentimes, such mutations give rise to the metastatic form of cancer, which is capable of moving throughout the body to distant sites. Adaptive immunity, including Cytolytic T cells (CTLs), are intended to kill tumours based on tumour specific antigen presented on Major Histocompatability Complex (MHC) Class I. However, metastatic tumours are capable of escaping such death through the downregulation of Antigen presentation machinery (APM), including MHC Class I. Here, we investigate the role of the small molecule Cannabigerol in upregulating class I MHC and altering cytokine signalling in metastatic tumours, and the consequent CTL tumour killing ex-vivo. Additionally, we determined the impact of Cannabigerol on immune infiltrates and tumour burden in vivo. Small molecules which enhance the efficacy of the CTL killing through upregulation of tumour-antigen bound MHC class I killing would be beneficial for therapeutic use through increased tumour killing, as well as reduced tumour metastasis, and would result in a prolonged host survival.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/69651
note: USE OF SMALL MOLECULE ACTIVITY IN UPREGULATION OF CLASS I MHC AND CHANGE IN TUMOUR CYTOKINE PROFILE IN METASTATIC MURINE EPITHELIAL CARCINOMA. by Sarah Dada B.Sc, Hon., Dalhousie University, 2016 A THESIS SUBMITTED IN PARTIAL FULFULLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Microbiology and Immunology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2019 © Sarah Dada, 2019 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis/dissertation entitled: Use of small molecule activity in upregulation of Class I MHC and change in tumour cytokine profile in metastatic murine epithelial carcinoma. submitted by Sarah Dada in partial fulfillment of the requirements for the degree of Master of Science in Microbiology and Immunology Examining Committee: Dr. Wilfred Jefferies Supervisor Dr. Raymond Andersen Supervisory Committee Member Dr. Nathan Lack Additional Examiner Additional Supervisory Committee Members: Dr. Marc Horwitz Supervisory Committee Member Dr. Michael Gold Supervisory Committee Member iii Abstract Cancer is a devastating disease that is responsible for the death of a quarter of Canadians. Cancer is the result of cells that gain genetic mutations or epigenetic changes that incur abnormal, uncontrolled proliferation. Oftentimes, such mutations give rise to the metastatic form of cancer, which is capable of moving throughout the body to distant sites. Adaptive immunity, including Cytolytic T cells (CTLs), are intended to kill tumours based on tumour specific antigen presented on Major Histocompatability Complex (MHC) Class I. However, metastatic tumours are capable of escaping such death through the downregulation of Antigen presentation machinery (APM), including MHC Class I. Here, we investigate the role of the small molecule Cannabigerol in upregulating class I MHC and altering cytokine signal…
has type: Thesis/Dissertation
date issued: 2019-05
is shown at: 10.14288/1.0378157
language: eng
degree discipline: Microbiology and Immunology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/69651