Comparison of MIN6 cell aggregate formation methods and shear resistance
department or school: Applied Science, Faculty of
department or school: Biomedical Engineering, School of
data provider: DSpace
degree campus: UBCV
creator: Kocyigit, Muzaffer Cagri
issued: 2018-12-21T16:49:11Z
issued: 2018
related degree: Master of Applied Science - MASc
degree grantor: University of British Columbia
description: Islet transplantation has the potential to cure type 1 diabetes, thereby avoiding the need for a lifetime of daily insulin injections. However, to protect the islet transplant from the recipients host immune system currently requires lifelong immunosuppression. Alginate gel microencapsulation is one of the biological envelopes being developed as a physical barrier to block rejection by the recipient immune system. When using emulsification for cell encapsulation, aggregates of cells are exposed to high shear stresses that can impact their recovery. This study has investigated the aggregation of MIN6 cells to develop a model system for insulin producing beta-cells. Two aggregation methods were investigated, either using a shaking agitation system or a static multi-well system without shaking. MIN6 cell aggregates generated by both methods were analyzed for their recovery after exposure to shear stresses. A disaggregation method was introduced to examine the cellular viability of the component cells in aggregates and the cells remained viable within aggregates. Finally, the aggregates were encapsulated using 1.5% (w/v) or 5% (w/v) alginate. The 5% alginate yielded higher encapsulation efficiencies and a more spherical structure with a narrower size distribution of capsule diameters, and so should be the more suitable choice for the further development of large-scale encapsulation production processing.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/68125
note: COMPARISON OF MIN6 CELL AGGREGATE FORMATION METHODS AND SHEAR RESISTANCE by Muzaffer Cagri Kocyigit B.Sc., Cumhuriyet University, 2010 M.Sc., Ankara University, 2014 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF APPLIED SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Biomedical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) December 2018 © Muzaffer Cagri Kocyigit, 2018 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Comparison of MIN6 Cell Aggregate Formation Methods and Shear Resistance submitted by Muzaffer Cagri Kocyigit in partial fulfillment of the requirements for the degree of Master of Applied Science In Biomedical Engineering Examining Committee: James Piret, Chemical and Biological Engineering Supervisor Christian Kastrup, Biochemistry and Molecular Biology Supervisory Committee Member Susan Baldwin, Chemical and Biological Engineering Additional Examiner iii Abstract Islet transplantation has the potential to cure type 1 diabetes, thereby avoiding the need for a lifetime of daily insulin injections. However, to protect the islet transplant from the recipients host immune system currently requires lifelong immunosuppression. Alginate gel microencapsulation is one of the biological envelopes being developed as a physical barrier to block rejection by the recipient immune system. When using emulsification for cell encapsulation, aggregates of cells are exposed to high shear stresses that can impact their recovery. This study has investigated the aggregation of MIN6 cells to develop a model system for insulin producing beta-cells. Two aggregation methods were investigated, either using a shaking agitation system or a static multi-well system without shaking. MIN6 cell aggregates generated by both methods were analyzed for their recovery after exposure to shear stresses. A disaggr…
has type: Thesis/Dissertation
date issued: 2019-02
is shown at: 10.14288/1.0375813
language: eng
degree discipline: Biomedical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/68125