Nicotine metabolism in the cabbage looper trichoplusia ni (Hübner)
department or school: Arts and Sciences, Irving K. Barber School of (Okanagan)
department or school: Biology, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Saremba, Brett
issued: 2018-04-09T21:02:37Z
issued: 2018
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Cabbage looper (Trichoplusia ni) larvae are generalist herbivores that feed on numerous cultivated plants. Consuming plant material from diverse plant species exposes these larvae to a wide variety of plant secondary metabolites involved in chemical defense against herbivory. The ability of the cabbage looper larvae to detoxify plant secondary metabolites, such as nicotine, has been attributed to the rapid excretion via the Malpighian (renal) tubules. However, the role of metabolism prior to excretion in the detoxification of nicotine in cabbage looper larvae has not been studied. The first objective of this thesis was to develop an accurate, precise, and sensitive method for the detection and quantification of nicotine and its major metabolites in tissues, blood and feces from insects. The second objective was to determine the appropriate nicotine dose to study the sub-lethal effects of nicotine in cabbage looper larvae. The third and final objective was to determine the metabolic fate of dietary nicotine in cabbage looper larvae. Previous studies have concluded that the cabbage looper does not metabolize nicotine. This thesis showed that the 4th instar larvae of the cabbage looper metabolized dietary nicotine into three distinct metabolites. In addition, the time course for nicotine metabolism and excretion was found to be more rapid than previously reported for other Lepidopteran insects. Taken together these data demonstrate that cabbage looper larvae are capable of efficiently metabolizing nicotine and excreting nicotine and these derived metabolites as a part of their xenobiotic coping mechanism.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/65230
note: Nicotine Metabolism in the Cabbage Looper Trichoplusia ni (Hübner) by Brett Saremba B.Sc., The University of British Columbia, 2015 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Biochemistry and Molecular Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) April 2018 © Brett Saremba, 2018 iiThe following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: Nicotine metabolism in the cabbage looper trichoplusia ni (hübner) submitted by Brett Saremba in partial fulfillment of the requirements of the degree of Master of Science. Mark Rheault, Irving K. Barber School of Arts and Sciences Supervisor Susan Murch, Irving K. Barber School of Arts and Sciences Supervisory Committee Member Karen Perry, Irving K. Barber School of Arts and Sciences Supervisory Committee Member Jeff Curtis, Irving K. Barber School of Arts and Sciences University Examiner iii Abstract Cabbage looper (Trichoplusia ni) larvae are generalist herbivores that feed on numerous cultivated plants. Consuming plant material from diverse plant species exposes these larvae to a wide variety of plant secondary metabolites involved in chemical defense against herbivory. The ability of the cabbage looper larvae to detoxify plant secondary metabolites, such as nicotine, has been attributed to the rapid excretion via the Malpighian (renal) tubules. However, the role of metabolism prior to excretion in the detoxification of nicotine in cabbage looper larvae has not been studied. The first objective of this thesis was to develop an accurate, precise, and sensitive method for the detection and quantification of nicotine and its major metabolites in tissues, blood and feces from insects. The second objective was to determine the appropriate nicotine dose to study the sub-lethal effects of nicotine in cabbage looper larvae. The third and final o…
has type: Thesis/Dissertation
date issued: 2018-05
is shown at: 10.14288/1.0365271
language: eng
degree discipline: Biochemistry and Molecular Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/65230