A biomechanical investigation of a dislocation spinal cord injury in a rat model
department or school: Applied Science, Faculty of
department or school: Biomedical Engineering, School of
data provider: DSpace
degree campus: UBCV
creator: Mattucci, Stephen Frank Ernesto
issued: 2017-12-22T23:41:36Z
issued: 2017
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Traumatic spinal cord injuries clinically occur in a heterogeneous fashion, including at different spinal levels, injury velocities, and injury mechanisms. Clinical treatment options, such as early surgical decompression produce inconsistent recovery outcomes in the patient population, despite demonstrating effectiveness in preclinical animal models. The most common biomechanical factors, such as cervical level, high-energy impact and dislocation injury mechanism, are not adequately represented in preclinical models, which may explain the lack of agreement between clinical studies. The overall objective of this thesis was to investigate the biomechanics of a high-speed cervical dislocation rat model at acute stages, refine the model, and incorporate residual compression. The temporal progression of acute SCI was investigated in different injury mechanisms, where dislocation injuries demonstrated the fastest loss of white matter tissue. To refine the dislocation model, new vertebral injury clamps were designed with a feature allowing the clamps to pivot and self-align when tightened. The vertebral kinematics during a dislocation injury were analysed using high-speed x-ray and clamp slippage was significantly reduced with the self-aligning clamps, compared to the existing clamps. This study also emphasized the importance of validating injury displacements against input parameters, particularly when comparing results or reproducing injuries. In order to implement residual compression within the dislocation model, injury parameters were independently investigated. Electrophysiology techniques were implemented to determine a minimum residual compression depth that affects signal conduction following a traumatic injury. Continuously holding the residual compression following the initial injury induced a significantly different physiological response compared to when the injury was immediately reduced. Behavioural outcome was used to identify severities following a range o…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/64175
note: A BIOMECHANICAL INVESTIGATION OF A DISLOCATION SPINAL CORD INJURY IN A RAT MODEL by Stephen Frank Ernesto Mattucci B.A.Sc., The University of British Columbia, 2008 M.A.Sc., The University of Waterloo, 2011 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Biomedical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) December 2017 © Stephen Frank Ernesto Mattucci, 2017 ii Abstract Traumatic spinal cord injuries clinically occur in a heterogeneous fashion, including at different spinal levels, injury velocities, and injury mechanisms. Clinical treatment options, such as early surgical decompression produce inconsistent recovery outcomes in the patient population, despite demonstrating effectiveness in preclinical animal models. The most common biomechanical factors, such as cervical level, high-energy impact and dislocation injury mechanism, are not adequately represented in preclinical models, which may explain the lack of agreement between clinical studies. The overall objective of this thesis was to investigate the biomechanics of a high-speed cervical dislocation rat model at acute stages, refine the model, and incorporate residual compression. The temporal progression of acute SCI was investigated in different injury mechanisms, where dislocation injuries demonstrated the fastest loss of white matter tissue. To refine the dislocation model, new vertebral injury clamps were designed with a feature allowing the clamps to pivot and self-align when tightened. The vertebral kinematics during a dislocation injury were analysed using high-speed x-ray and clamp slippage was significantly reduced with the self-aligning clamps, compared to the existing clamps. This study also emphasized the importance of validating injury displacements against input parameters, particularly when comparing results or reproducing injuries. In order to implement residual compressi…
has type: Thesis/Dissertation
date issued: 2018-02
is shown at: 10.14288/1.0362410
language: eng
degree discipline: Biomedical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/64175