Development of advanced operators for enhanced on-chip biosensing in digital microfluidic platforms
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Samiei, Ehsan
issued: 2017-01-21T03:58:31
issued: 2016
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Over the past two decades, digital microfluidics (DMF) has grown significantly as a powerful tool for lab-on-a-chip (LOC) applications. Since the introduction of DMF with its primary capabilities in sample manipulation (transport, splitting and mixing) in the droplet format, many advances have been made towards the development of platforms capable of performing entire processes of biochemical assays in an automated fashion. These advances include the progress made towards the fabrication methods, and the development of techniques for sample manipulation and integration of biosensors. Despite the general success in such developments, DMF still lacks capabilities in sample manipulation (including the droplets and their contents, e.g. cells and microbeads), specifically for biosensing, in which sample preparation and post-sensing removal of the sample are required. Therefore, in majority of the applications, DMF has been used for processing parts of the entire assay, and after biosensing, the recovery of the chip was hindered due to the contamination of the biosensor for its hydrophilic behavior. This thesis aims at the development of advanced operators for accurate pre-sensing sample preparation, biosensing and post sensing sample removal. For this purpose, an unequal droplet splitting method is developed based on the geometrical modification of one actuating electrode, which enables dispensing/splitting droplets with a wide range of volumes and with an accuracy of over 99%. An electrohydrodynamic technique is developed for rapid mixing inside the stationary droplets, enhancing the mixing time and eliminating the need for frequent and cyclic transport of the droplet on the chip. A dielectrophoretic-gravity driven technique is developed for concentrating and focusing the particles and cells inside the sample droplet. Also, a systematic study has been performed on the surface properties and geometry of the biosensors to optimize their geometry and configuration on DMF d…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/60122
note: Development of advanced operators for enhanced on-chip biosensing in digital microfluidic platforms by Ehsan Samiei B.Sc., Urmia University, Iran, 2007 M.Sc., K. N. Toosi University of Technology (KNTU), Iran, 2010 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE COLLEGE OF GRADUATE STUDIES (Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) December 2016 © Ehsan Samiei, 2016 The undersigned certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis entitled: Development of advanced operators for enhanced on-chip biosensing in digital microfluidic platforms Submitted by Ehsan Samiei in partial fulfillment of the requirements of The degree of Doctor of Philosophy . Dr. Mina Hoorfar, School of Engineering, The University of British Columbia Supervisor, Professor (please print name and faculty/school above the line) Dr. Homayoun Najjaran, School of Engineering, The University of British Columbia Supervisory Committee Member, Professor (please print name and faculty/school in the line above) Dr. Vladan Prodanovic, Department of Chemical and Biological Engineering, The University of British Columbia Supervisory Committee Member, Professor (please print name and faculty/school in the line above) Dr. Ian Foulds, School of Engineering, The University of British Columbia University Examiner, Professor (please print name and faculty/school in the line above) Dr. Ali Dolatabadi, Department of Mechanical and Industrial Engineering, Concordia University External Examiner, Professor (please print name and university in the line above) December 13, 2016 (Date submitted to Grad Studies) ii Abstract Over the past two decades, digital microfluidics (DMF) has grown significantly as a powerful tool for lab-on-a-chip (LOC) applications. Since the introduction of DMF with its primary capabilities in sample manipulation (transport, splitting and mixing) in the droplet format, man…
has type: Thesis/Dissertation
date issued: 2017-02
is shown at: 10.14288/1.0340483
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/60122