Enhancement of specialized metabolism, regeneration efficiency and biological activity in lavandin (Lavandula x intermedia cv 'Grosso')
department or school: Arts and Sciences, Irving K. Barber School of (Okanagan)
department or school: Biology, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Erland, Lauren Alexandra Elizabeth
issued: 2016-01-01T00:00:00Z
issued: 2015
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: This study aimed to improve essential oil composition by modifying terpene production in Lavandula x intermedia cv Grosso via mutagenesis to more closely resemble the oil of the commercially valuable essential oil from L. angustifolia or the medicinally active essential oil from L. latifolia. Additionally this study aimed to identify genes that control essential oil production in lavenders, and to determine the effect of essential oil composition on biological activity, specifically insecticidal and insect repellent properties. This study resulted in an improved method for the efficient regeneration of Grosso lavender, and applied this method to generate ten unique mutants. The transcriptomes of some mutants were sequenced, and thirty seven differentially expressed transcripts were identified as being involved in the biosynthesis and production of essential oil terpenes. The transcript expression results were confirmed by real-time quantitative polymerase chain reaction analysis. The lavender essential oil showing greatest biological activity against an invasive pest, spotted wing drosophila, was identified as Lavandula latifolia cv Medikus and the active constituents were identified through fumigation and spray toxicity assays as the monoterpenes 1,8-cineole and linalool. These oils showed strong fumigation and contact toxicity. In all, this thesis presents the generation, screening and analysis of unique L. x intermedia essential oil mutants, which represent both potential new commercial cultivars and model organisms for the investigation of the regulation and biosynthesis of essential oil terpenes.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/52707
note: ENHANCEMENT OF SPECIALIZED METABOLISM, REGENERATION EFFICIENCY AND BIOLOGICAL ACTIVITY IN LAVANDIN (LAVANDULA X INTERMEDIA CV ‘GROSSO’) by Lauren Alexandra Elizabeth Erland B.Sc., University of British Columbia, 2012 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Biochemistry and Molecular Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) March 2015 ©Lauren Alexandra Elizabeth Erland, 2015 ii Abstract This study aimed to improve essential oil composition by modifying terpene production in Lavandula x intermedia cv Grosso via mutagenesis to more closely resemble the oil of the commercially valuable essential oil from L. angustifolia or the medicinally active essential oil from L. latifolia. Additionally this study aimed to identify genes that control essential oil production in lavenders, and to determine the effect of essential oil composition on biological activity, specifically insecticidal and insect repellent properties. This study resulted in an improved method for the efficient regeneration of Grosso lavender, and applied this method to generate ten unique mutants. The transcriptomes of some mutants were sequenced, and thirty seven differentially expressed transcripts were identified as being involved in the biosynthesis and production of essential oil terpenes. The transcript expression results were confirmed by real-­‐time quantitative polymerase chain reaction analysis. The lavender essential oil showing greatest biological activity against an invasive pest, spotted wing drosophila, was identified as Lavandula latifolia cv Medikus and the active constituents were identified through fumigation and spray toxicity assays as the monoterpenes 1,8-­‐cineole and linalool. These oils showed strong fumigation and contact toxicity. In all, this thesis presents the generation, screening and analysis of unique L. x intermedia essential oil mutants, which represent both potential…
has type: Thesis/Dissertation
date issued: 2015-05
is shown at: 10.14288/1.0074431
language: eng
degree discipline: Biochemistry and Molecular Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/52707