The role of somatostatin expression in hair follicle immune privilege
department or school: Medicine, Faculty of
department or school: Medicine, Department of
data provider: DSpace
degree campus: UBCV
creator: Breitkopf, Trisia
issued: 2012-04-13T19:53:49Z
issued: 2012
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: The hair follicle is a mini-organ, consisting of many different types and groups of cells, capable of frequent remodeling and cycles of growth. Immune privilege (IP) is believed to exist in the anagen growth stage of the hair follicle (HF). Previous studies using immunohistology have illustrated unique downregulation of major histocompatibility complex Class I in the HF bulb, as well as expression of immunosuppressive factors in the bulge region. However, quantitative studies and functional studies to clearly demonstrate IP in HF cells are required. My goal was to examine the middle (hair fiber and sheaths) and lower (bulb) portion of the human HF to identify a novel functional mechanism of IP. My hypothesis was that the bulb and the middle third of the hair follicle have functional immune privilege capabilities. In an in vitro experiment, I found that HF cells appeared to suppress histo-incompatible peripheral blood mononuclear cell (PBMC) IFN-gamma secretion relative to epidermal cells. I screened expression of IP-related genes in HFs relative to interfollicular epidermis by quantitative real-time RT-PCR. Briefly, I found significant downregulation of all Class I and Class II HLAs examined in the bulb and sheaths. There were also several genes coding for immunosuppressant secretory factors significantly upregulated in the sheath. Most notably, somatostatin (SST) was significantly upregulated in the sheath 5.9-fold and in the bulb 94.2-fold relative to non-follicular epidermis. This led me to investigate the hypothesis that SST contributes to IP in hair follicles. I found strong expression of SST in the outer root sheath by immunohistochemistry and significant secretion of SST from HF sheath cells compared to epidermal cells in culture. PBMCs cultured with allogeneic immuno-stimulatory epidermal cells and SST secreted significantly less IFN-gamma than controls. Additionally, a SST antagonist drug appeared to interfere with the immunosuppressive effect of sheath cel…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/41992
note: THE ROLE OF SOMATOSTATIN EXPRESSION IN HAIR FOLLICLE IMMUNE PRIVILEGE by Trisia Breitkopf A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE STUDIES (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2012 © Trisia Breitkopf, 2012 Abstract The hair follicle is a mini-organ, consisting of many different types and groups of cells, capable of frequent remodeling and cycles of growth. Immune privilege (IP) is believed to exist in the anagen growth stage of the hair follicle (HF). Previous studies using immunohistology have illustrated unique downregulation of major histocompatibility complex Class I in the HF bulb, as well as expression of immunosuppressive factors in the bulge region. However, quantitative studies and functional studies to clearly demonstrate IP in HF cells are required. My goal was to examine the middle (hair fiber and sheaths) and lower (bulb) portion of the human HF to identify a novel functional mechanism of IP. My hypothesis was that the bulb and the middle third of the hair follicle have functional immune privilege capabilities. In an in vitro experiment, I found that HF cells appeared to suppress histo-incompatible peripheral blood mononuclear cell (PBMC) IFN-gamma secretion relative to epidermal cells. I screened expression of IP-related genes in HFs relative to interfollicular epidermis by quantitative real-time RT-PCR. Briefly, I found significant downregulation of all Class I and Class II HLAs examined in the bulb and sheaths. There were also several genes coding for immunosuppressant secretory factors significantly upregulated in the sheath. Most notably, somatostatin (SST) was significantly upregulated in the sheath 5.9-fold and in the bulb 94.2-fold relative to non-follicular epidermis. This led me to investigate the hypothesis that SST contributes to IP in hair follicles. I found strong expression of SST in the outer root sheath by immunohi…
has type: Thesis/Dissertation
date issued: 2012-05
is shown at: 10.14288/1.0072696
language: eng
degree discipline: Experimental Medicine
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/41992