Capillary electrophoresis-mass spectrometry separation of isomeric biological compounds
department or school: Science, Faculty of
department or school: Chemistry, Department of
data provider: DSpace
degree campus: UBCV
creator: Soliman, Laiel
issued: 2012-10-16T20:35:31Z
issued: 2012
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Current prostate cancer (PCa) diagnosis based on prostate-specific antigen (PSA) has been gradually losing its credibility over the last decade due to contradictory results in published literature and clinical practice. Recently, a group of potential PCa biomarkers in urine, particularly sarcosine, was found to increase significantly as the cancer progressed to metastasis. In Chapter 2, we report a simple, robust, and reproducible capillary electrophoresis–electrospray ionization–tandem mass spectrometry (CE–ESI-MS/MS) method for the determination of sarcosine and other representative potential biomarkers in pooled urine. A solid phase extraction (SPE) technique was optimized for maximum recovery of sarcosine. With no derivatization step, excellent resolution between sarcosine and its isomers (α-alanine and β-alanine) was achieved. A separate non-SPE method was also developed for quantitative determination of highly concentrated urinary metabolites. Precision for intra- and inter-day standard addition calibration of sarcosine were found to be within 15%, whereas intra-day precisions for the rest of the metabolites varied from 0.03 to 13.4%. Acceptable intra-day and inter-day accuracies, ranging from 80 to 124%, were obtained for sarcosine and the other metabolites. The second part of the thesis takes on a more challenging task. The importance of chiral separation in pharmaceutical, agriculture, and food industries has driven separation scientists to develop more powerful methodologies in conjunction with the structural capabilities of mass spectrometry. In Chapter 3, chiral separation of D- and L-tryptophan was compared on a bare-fused silica capillary and a PEI-coated capillary. Although a higher resolution was observed for uncoated capillaries, analytes were found to migrate slower resulting to longer analysis times (tm > 20 min). With shorter migration times (tm < 10 min) and acceptable resolution, further investigations on different factors that could affect ena…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/43419
note: CAPILLARY ELECTROPHORESIS–MASS SPECTROMETRY SEPARATION OF ISOMERIC BIOLOGICAL COMPOUNDS by Laiel Soliman B.Sc., Thompson Rivers University, 2007 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE MASTER OF SCIENCE in The Faculty of Graduate Studies (Chemistry) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2012  Laiel Soliman, 2012 ii ABSTRACT Current prostate cancer (PCa) diagnosis based on prostate-specific antigen (PSA) has been gradually losing its credibility over the last decade due to contradictory results in published literature and clinical practice. Recently, a group of potential PCa biomarkers in urine, particularly sarcosine, was found to increase significantly as the cancer progressed to metastasis. In Chapter 2, we report a simple, robust, and reproducible capillary electrophoresis–electrospray ionization–tandem mass spectrometry (CE–ESI-MS/MS) method for the determination of sarcosine and other representative potential biomarkers in pooled urine. A solid phase extraction (SPE) technique was optimized for maximum recovery of sarcosine. With no derivatization step, excellent resolution between sarcosine and its isomers (α-alanine and β-alanine) was achieved. A separate non-SPE method was also developed for quantitative determination of highly concentrated urinary metabolites. Precision for intra- and inter-day standard addition calibration of sarcosine were found to be within 15%, whereas intra-day precisions for the rest of the metabolites varied from 0.03 to 13.4%. Acceptable intra-day and inter-day accuracies, ranging from 80 to 124%, were obtained for sarcosine and the other metabolites. The second part of the thesis takes on a more challenging task. The importance of chiral separation in pharmaceutical, agriculture, and food industries has driven separation scientists to develop more powerful methodologies in conjunction with the structural capabilities of mass spectrometry. In Chapter 3, chiral separation of D- and…
has type: Thesis/Dissertation
date issued: 2012-11
is shown at: 10.14288/1.0062319
language: eng
degree discipline: Chemistry
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivs 3.0 Unported
rights URI: http://creativecommons.org/licenses/by-nc-nd/3.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/43419