Construction of dual-beam optical tweezer for single-molecule study
department or school: Science, Irving K. Barber Faculty of (Okanagan)
department or school: Chemistry, Department of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: An, Minh (Scott)
issued: 2024-10-29T16:06:30Z
issued: 2024
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Optical tweezer is a well-established and continually evolved microscopic trapping and manipulation technique widely employed across numerous disciplines. A tightly focused Gaussian-profile laser can be used to confine homogenous particles by exerting two forces: 1. Scattering force, which maintains the target particles along the axis of the laser, and 2. Gradient forces prevent the particles from laterally drifting off the beam. We constructed a dual-beam variant of the optical tweezer for single-molecule studies. This system allows the simultaneous trapping of two particles, where one serves as an anchor point and the other moves to stretch DNA-based nanostructures. The decoupling from surface anchoring enables the system to achieve sub-piconewton resolution measurement with minimal environmental noise. We developed in-house programs facilitating data acquisition and control of various components. This allows rapid testing and deployment of custom features tailored for different experiment types and DNA structures. Finally, we established comprehensive user protocols and calibrated the system force measurement. The development of this instrument provides a powerful approach for future investigators in single molecular and cellular studies.
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/89571
note: CONSTRUCTION OF DUAL-BEAM OPTICAL TWEEZER FOR SINGLE-MOLECULE STUDY by Minh (Scott) An B. Sc. Hons. Biochemistry, The University of British Columbia Okanagan, 2019 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Biochemistry & Molecular Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) October 2024 © Minh (Scott) An, 2024 ii The following individuals certify that they have read, and recommend to the College of Graduate Studies for acceptance, a thesis/dissertation entitled: CONSTRUCTION OF DUAL-BEAM OPTICAL TWEEZER FOR SINGLE-MOLECULE STUDY submitted by Minh (Scott) An in partial fulfillment of the requirements of the degree of Master of Science in Biochemistry & Molecular Biology . Dr. Isaac T.S. Li, Department of Chemistry, Irving K. Barber Faculty of Science Supervisor Dr. Robert Godin, Department of Chemistry, Irving K. Barber Faculty of Science Supervisory Committee Member Dr. David Jack, Department of Chemistry, Irving K. Barber Faculty of Science Supervisory Committee Member Dr. Andrew Jirasek, Department of Computer Science, Mathematics, Physics and Statistics, Irving K. Barber Faculty of Science University Examiner iii ABSTRACT Optical tweezer is a well-established and continually evolved microscopic trapping and manipulation technique widely employed across numerous disciplines. A tightly focused Gaussian-profile laser can be used to confine homogenous particles by exerting two forces: 1. Scattering force, which maintains the target particles along the axis of the laser, and 2. Gradient forces prevent the particles from laterally drifting off the beam. We constructed a dual-beam variant of the optical tweezer for single-molecule studies. This system allows the simultaneous trapping of two particles, where one serves as an anchor point and the other moves to stretch DNA-based nanostructures. The decoupling from surface anchoring enables the system to achieve sub-picon…
has type: Thesis/Dissertation
date issued: 2025-02
is shown at: 10.14288/1.0447170
language: eng
degree discipline: Biochemistry and Molecular Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Li, Isaac T. S.
type: Text
identifier URI: http://hdl.handle.net/2429/89571