Genetic analysis of defense pathway downstream of the immune receptor SNC2 in Arabidopsis thaliana
department or school: Science, Faculty of
department or school: Botany, Department of
data provider: DSpace
degree campus: UBCV
creator: Wang, Yiran
issued: 2025-10-31T07:00:00Z
issued: 2024
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Plants face constant threats from various microbial pathogens. Successful sensing and defending against infections are essential to their survival. Arabidopsis thaliana receptor like protein SUPPRESSOR OF NPR1-1, CONSTITUTIVE 2 (SNC2) is of great importance to plant basal immunity. The transcription factors CALMODULIN-BINDING PROTEIN 60g (CBP60g) and SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) define two parallel signaling pathways downstream of SNC2 to activate defense responses. In my PhD research, I aimed to identify the components that are involved in the CBP60g-dependent signaling pathway downstream of SNC2. From a forward genetic screen, mutants with suppressed autoimmunity of sard1-1 snc2-1D were isolated. Characterization of suppressor mutant 125-1 revealed that Epsin-like clathrin adaptor ECA4 is an inhibiting factor of SNC2-mediated immunity. A gain-of-function allele eca4-4D or overexpression of ECA4 leads to enhanced susceptibility against pathogens. Transient expression of ECA4 or eca4-4D in Nicotiana benthamiana reduces the protein levels of BDA1, a transmembrane protein required for SNC2-mediated immunity. Additionally, eca4-4D partially suppresses the autoimmunity of bda1-17D. Taken together, ECA4 contributes to SNC2-mediated immunity by negatively modulating the protein level of BDA1. 116-1 is a partial suppressor of sard1-1 snc2-1D autoimmunity, which carries a premature stop codon in ZINC-FINGER AND OCRE DOMAIN-CONTAINING PROTEIN 1 (ZOP1). ZOP1 encodes a pre-mRNA splicing factor that is involved in gene regulation. zop1 mutant exhibits enhanced susceptibility against pathogens. Moreover, many defense-related genes, including BDA1, were downregulated in the zop1 plants. Altogether, ZOP1 positively contributes to plant immunity through regulating the transcript levels of defense related genes. Studies of the suppressor mutant 123-1 uncovered the role of deubiquitinating enzyme ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM 1 (AMSH1) in SNC2-me…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/89445
note: GENETIC ANALYSIS OF DEFENSE PATHWAY DOWNSTREAM OF THE IMMUNE RECEPTOR SNC2 IN ARABIDOPSIS THALIANA by Yiran Wang B. Sc., China Agricultural University, 2018 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Botany) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) October 2024 © Yiran Wang, 2024 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Genetic analysis of defense pathway downstream of the immune receptor SNC2 in Arabidopsis thaliana submitted by Yiran Wang in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Botany Examining Committee: Dr. Yuelin Zhang, Professor, Botany, UBC Supervisor Dr. George Haughn, Professor Emeritus, Botany, UBC Supervisory Committee Member Dr. Richard Hamelin, Professor, Forest Conservation Sciences, UBC University Examiner Dr. Siyun Wang, Professor, Land and Food Systems, UBC University Examiner Additional Supervisory Committee Members: Dr. Xin Li, Professor, Botany, UBC Co-Supervisor Dr. Liang Song, Assistant Professor, Botany, UBC Supervisory Committee Member iii Abstract Plants face constant threats from various microbial pathogens. Successful sensing and defending against infections are essential to their survival. Arabidopsis thaliana receptor like protein SUPPRESSOR OF NPR1-1, CONSTITUTIVE 2 (SNC2) is of great importance to plant basal immunity. The transcription factors CALMODULIN-BINDING PROTEIN 60g (CBP60g) and SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) define two parallel signaling pathways downstream of SNC2 to activate defense responses. In my PhD research, I aimed to identify the components that are involved in the CBP60g-dependent signaling pathway downstream of SNC2. From a forward genetic screen, mutants with suppressed autoimmunity of sard1-1 snc2-1D were isolate…
has type: Thesis/Dissertation
date issued: 2024-11
is shown at: 10.14288/1.0445599
language: eng
degree discipline: Botany
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
contributor: Zhang, Yuelin
contributor: Li, Xin
type: Text
identifier URI: http://hdl.handle.net/2429/89445