Interpreting species, intraspecific and intra-individual variability by comprehensively characterizing a fishâs respiratory phenotype with valid measures of oxygen uptake
department or school: Land and Food Systems, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Zhang, Yangfan
issued: 2021-04-12T07:00:00Z
issued: 2021
related degree: Doctor of Philosophy - PhD
degree grantor: University of British Columbia
description: Vertebrate life is sustained by aerobic metabolism and temporary bouts of anaerobic metabolism. Steady-state aerobic metabolism can be assessed by measuring whole-organism oxygen uptake (á¹Oâ), the core of Fryâs paradigm, which becomes a part of a respiratory phenotype that also includes anaerobic capacity. In fishes, environment profoundly affects respiratory phenotype, perhaps even determining migratory and reproductive successes, partially driven by hypoxia, increased thermal and pathogen load. Understanding how such factors affect capacity, functional costs and mortality needs a more accurate, precise, and comprehensive characterization of a fishâs respiratory phenotype. Hence, I developed an Integrated Respiratory Assessment Paradigm (IRAP) to characterize a respiratory phenotype (its needs, capacities & patterns) for individual fish that responds to Fryâs paradigm (controlling, limiting, masking, directive, and lethal factors). Chapter 2 describes an IRAP protocol that measures 14 metrics in 8 fish simultaneously over 3 days, a high sampling throughput to better bridge physiology-&-ecology. Chapter 3 introduces and validates new analytical approaches that improved the resolution and accuracy of maximum á¹Oâ (á¹Oâmax) estimations in aquatic respirometry. Chapter 4 illustrates the detection sensitivity of the refined IRAP by distinguishing respiratory phenotypes among three rainbow trout strains using differences in á¹Oâmax, absolute aerobic capacity, critical oxygen saturation (Oâcrit) and hypoxia tolerance. Moreover, high aerobic and anaerobic capacities correlated with high hypoxic and thermal tolerances among these strains. Chapter 5 explores how acclimation to hypoxia affects the respiratory phenotype, discovering hypoxia-acclimated European sea bass suppress standard metabolic rate (SMR) and Oâcrit, a respiratory phenotype better suited for exploiting hypoxic habitats. Chapter 6 explores the diagnostic power of the refined IRAP by assessing the load-depend…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/77767
note: INTERPRETING SPECIES, INTRASPECIFIC AND INTRA-INDIVIDUAL VARIABILITY BY COMPREHENSIVELY CHARACTERIZING A FISHâS RESPIRATORY PHENOTYPE WITH VALID MEASURES OF OXYGEN UPTAKE by Yangfan Zhang B.Sc. (1st Honors), Dalhousie University, 2014 M.Sc. The University of British Columbia, 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Applied Animal Biology) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2021 © Yangfan Zhang, 2021 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the dissertation entitled: Interpreting species, intraspecific and intra-individual variability by comprehensively characterizing a fishâs respiratory phenotype with valid measures of oxygen uptake submitted by Yangfan Zhang in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Applied Animal Biology Examining Committee: Anthony Farrell, Department of Zoology & Faculty of Land and Food System, University of British Columbia, Canada Supervisor Marina Von Keyserlingk, Animal Welfare, Faculty of Land and Food System, University of British Columbia, Canada Supervisory Committee Member Denis Chabot, Maurice Lamontagne Institute, Fisheries and Oceans Canada Supervisory Committee Member Jeffery Richards, Department of Zoology, University of British Columbia, Canada University Examiner William Sheel, School of Kinesiology, Faculty of Education, University of British Columbia, Canada University Examiner Additional Supervisory Committee Members: Guy Claireaux, Laboratoire des Sciences de, Université de Bretagne Occidentale & Cabinet d'Annick Girardin, ministre de la mer, France Co-Supervisor iii Abstract Vertebrate life is sustained by aerobic metabolism and temporary bouts of anaerobic metabolism. Steady-state aerobic metabolism can be assessed by measuring whole-organism oxy…
has type: Thesis/Dissertation
date issued: 2021-05
is shown at: 10.14288/1.0396683
language: eng
degree discipline: Applied Animal Biology
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/77767