Optimization of electrochemical microfluidic biosensor design for point of care devices
department or school: Applied Science, Faculty of
department or school: Engineering, School of (Okanagan)
data provider: DSpace
degree campus: UBCO
creator: Rellstab Sánchez, Pamela Inés
issued: 2020-05-08T16:16:33Z
issued: 2020
related degree: Master of Applied Science - MASc
degree grantor: University of British Columbia
description: Over the last decade, there has been an ever-increasing interest in microfluidic biosensors in the biomedical sector, especially for diagnosing infectious diseases. These are still one of the top ten causes of mortality around the world and for regions with limited resources where mortality rates are the highest, it is imperative to provide an on-site diagnosis. With early diagnosis, accurate treatments are possible, which can help contain and prevent disease outbreaks. However, there are still several challenges that need to be addressed to develop accurate and sensitive Point of Care (POC) detection devices. Improvements in sample processing, flow manipulation and signal amplification can support powerful emerging technologies for the development of fast, inexpensive, portable, easy-to-use and sensitive diagnostic devices. For the purpose of miniaturization, simple instrumentation, low production cost, and rapid response coupled with high sensitivity/selectivity, microfluidic DNA biosensors with an electrochemical transducer have shown great promise. This thesis focuses on experiments that aim to optimize three design aspects of an electrochemical microfluidic biosensor to improve its applicability: 1) flow cell geometry, 2) flow rate and 3) electrohydrodynamic mixing. Numerical simulations were carried out to model the fluid and particle behavior inside the detection cell. Understanding these flow characteristics led to the development of a new geometry providing uniform distribution of the sample flow across the detection area for increasing the biosensorâs efficiency. In addition, the identification of an optimal flow rate was required to analyze a higher sample volume. To achieve this, the binding efficiency of Streptavidin and Methylene Blue-Biotin was compared at five different flow rates with Square Wave Voltammetry. This resulted in finding an alternate flow rate that could increase the volume sample without sacrificing the selectivity/sensitivity of the d…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/74373
note: Optimization of Electrochemical Microfluidic Biosensor Design for Point of Care Devices by Pamela Inés Rellstab Sánchez B.Sc., Tecnológico de Monterrey, Mexico, 2019 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF APPLIED SCIENCE in THE COLLEGE OF GRADUATE STUDIES (Mechanical Engineering) THE UNIVERSITY OF BRITISH COLUMBIA (Okanagan) April 2020 © Pamela Inés Rellstab Sánchez, 2020 ii The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, a thesis/dissertation entitled: Optimization of Electrochemical Microfluidic Biosensor Design for Point of Care Devices submitted by Pamela Ines Rellstab Sanchez in partial fulfillment of the requirements for the degree of Master of Applied Sciences in Mechanical Engineering Examining Committee: Dr. Mina Hoorfar, School of Engineering, Faculty of Applied Sciences Co-supervisor Dr. Homayoun Najjaran, School of Engineering, Faculty of Applied Sciences Co-supervisor Dr. Isaac Li, Department of Chemistry, Irving K. Barber School of Arts and Sciences Supervisory Committee Member Dr. Ray Taheri, School of Engineering, Faculty of Applied Sciences Supervisory Committee Member Dr. Shahria Alam, School of Engineering, Faculty of Applied Sciences University Examiner iii Abstract Over the last decade, there has been an ever-increasing interest in microfluidic biosensors in the biomedical sector, especially for diagnosing infectious diseases. These are still one of the top ten causes of mortality around the world and for regions with limited resources where mortality rates are the highest, it is imperative to provide an on-site diagnosis. With early diagnosis, accurate treatments are possible, which can help contain and prevent disease outbreaks. However, there are still several challenges that need to be addressed to develop accurate and sensitive Point of Care (POC) detection devices. Improvements in sample processi…
has type: Thesis/Dissertation
date issued: 2020-05
is shown at: 10.14288/1.0390429
language: eng
degree discipline: Mechanical Engineering
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/74373