Effect of ethylenediaminetetraacetic acid pre-treatment on biofilm dissolution by sodium hypochlorite
department or school: Dentistry, Faculty of
data provider: DSpace
degree campus: UBCV
creator: Ananthan, Joshua
issued: 2018-12-03T18:07:33Z
issued: 2018
related degree: Master of Science - MSc
degree grantor: University of British Columbia
description: Introduction: Endodontic treatment is aimed at the management and treatment of pathoses caused by bacterial colonization of the root canal system; this is primarily achieved through mechanical and chemical means. Various irrigants have been introduced in varying degrees, concentrations and combinations to optimize bacterial eradication, however, there is no well established optimal protocol nor a recommended procedure by a regulatory body. The present study aims to evaluate the effect of ethylenediaminetetraacetic acid (EDTA) pre-treatment on sodium hypochloriteâs ability to dissolve biofilm. Materials and Methods: Hydroxyapatite discs were inoculated with subgingival plaque and biofilm was grown in brain-heart infusion broth for three weeks in anaerobic conditions. Samples were exposed to a pre-treatment of either sterile water or 17% EDTA for 30 or 60 seconds, washed with an intermediate sterile water rinse, and subjected to either 2% or 6% sodium hypochlorite. Samples were examined under stereomicroscopy for complete dissolution of the biofilm; time was recorded from initial exposure to sodium hypochlorite to complete dissolution. Results: Biofilm dissolution time was decreased by increasing the concentration of sodium hypochlorite from 2% to 6% for all matched groups. Pre-treatment with 17% EDTA for 30 seconds decreased the time for biofilm dissolution by sodium hypochlorite while pre-treatment with 17% EDTA for 60 seconds increased the time for biofilm dissolution; this effect was conserved between both 2% and 6% concentrations of sodium hypochlorite. Discussion: Overall, pre-treatment of biofilms with 17% EDTA will alter sodium hypochloriteâs dissolution ability. Shorter exposure times may augment NaOCl biofilm dissolution while long exposure times may be detrimental to sodium hypochloriteâs dissolution ability. Regardless, of length of pre-treatment time, sodium hypochlorite at both 2% and 6% concentrations was eventually able to complete dissolve all biofilm…
aggregated CHO: https://circle.library.ubc.ca/rest/handle/2429/67956
note: EFFECT OF ETHYLENEDIAMINETETRAACETIC ACID PRE-TREATMENT ON BIOFILM DISSOLUTION BY SODIUM HYPOCHLORITE by Joshua Ananthan D.D.S. University of Toronto 2016 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Craniofacial Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) August 2018 © Joshua Ananthan, 2018 The following individuals certify that they have read, and recommend to the Faculty of Graduate and Postdoctoral Studies for acceptance, the thesis entitled: Effect of ethylenediaminetetraacetic acid pre-treatment on biofilm dissolution by sodium hypochlorite ! submitted by Joshua Ananthan in partial fulfillment of the requirements for the degree of Master of Science â¨in Craniofacial Science Examining Committee: Dr. Markus Haapasalo, Department of Oral Biological & Medical Sciences Supervisor Dr. Ya Shen, Department of Oral Biological & Medical Sciences Co-Supervisor Dr. Adriana Manso, Department of Oral Biological & Medical Sciences Supervisory Committee Member Dr. Vincent Lee, Department of Oral Health Sciences Additional Examiner 2iiAbstract Introduction: Endodontic treatment is aimed at the management and treatment of pathoses caused by bacterial colonization of the root canal system; this is primarily achieved through mechanical and chemical means. Various irrigants have been introduced in varying degrees, concentrations and combinations to optimize bacterial eradication, however, there is no well established optimal protocol nor a recommended procedure by a regulatory body. The present study aims to evaluate the effect of ethylenediaminetetraacetic acid (EDTA) pre-treatment on sodium hypochloriteâs ability to dissolve biofilm. Materials and Methods: Hydroxyapatite discs were inoculated with subgingival plaque and biofilm was grown in brain-heart infusion broth for three weeks in anaerobic conditions. Samples were exposed to a pre-treatment of either steri…
has type: Thesis/Dissertation
date issued: 2019-02
is shown at: 10.14288/1.0374945
language: eng
degree discipline: Craniofacial Science
provider: Vancouver : University of British Columbia Library
publisher: University of British Columbia
rights: Attribution-NonCommercial-NoDerivatives 4.0 International
rights URI: http://creativecommons.org/licenses/by-nc-nd/4.0/
scholar level: Graduate
type: Text
identifier URI: http://hdl.handle.net/2429/67956