Open Collections

UBC Research Data

In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation and exacerbation of inflammation Charlotte A. Clayton; Imogen Porter; Brian D. Deng; Giselle McCallum; Apsara Srinivas; Claire Sie; Katharine M. Ng; Jerry Y. He; Alexander Pei; Dominique Tertigas; Deanna M. Pepin; Touran Fardeen; Sidhartha R. Sinha; Michael G. Surette; Bruce A. Vallance; Carolina Tropini

Description

In the United States an estimated 14 million colonoscopies are performed yearly, each requiring patients to undergo bowel preparation, a laxative cleansing of the intestine’s luminal contents. Despite its widespread use, the effects of bowel preparation on gut physiology and susceptibility to pathogens remains poorly understood, particularly in individuals with compromised gut health. Using mouse and in vitro models, we found that bowel preparation with the laxative polyethylene glycol (PEG) rapidly disrupts, transiently increasing susceptibility to infection by Salmonella Typhimurium, including a non-motile mutant, and by gut pathobionts derived from ulcerative colitis microbiota. Bowel preparation also facilitated bacterial translocation to extraintestinal sites (mesenteric lymph nodes, liver, and spleen) and exacerbated inflammation in a chemically-induced colitis model. Although these findings are preclinical, they suggest that bowel preparation may have underappreciated risks in vulnerable populations, and warrant further clinical investigation.

Item Metadata

Title	In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation and exacerbation of inflammation
Creator	Charlotte A. Clayton; Imogen Porter; Brian D. Deng; Giselle McCallum; Apsara Srinivas; Claire Sie; Katharine M. Ng; Jerry Y. He; Alexander Pei; Dominique Tertigas; Deanna M. Pepin; Touran Fardeen; Sidhartha R. Sinha; Michael G. Surette; Bruce A. Vallance; Carolina Tropini
Contributor	Brian D. Deng
Date Issued	2025-10-09
Description	In the United States an estimated 14 million colonoscopies are performed yearly, each requiring patients to undergo bowel preparation, a laxative cleansing of the intestine’s luminal contents. Despite its widespread use, the effects of bowel preparation on gut physiology and susceptibility to pathogens remains poorly understood, particularly in individuals with compromised gut health. Using mouse and in vitro models, we found that bowel preparation with the laxative polyethylene glycol (PEG) rapidly disrupts, transiently increasing susceptibility to infection by Salmonella Typhimurium, including a non-motile mutant, and by gut pathobionts derived from ulcerative colitis microbiota. Bowel preparation also facilitated bacterial translocation to extraintestinal sites (mesenteric lymph nodes, liver, and spleen) and exacerbated inflammation in a chemically-induced colitis model. Although these findings are preclinical, they suggest that bowel preparation may have underappreciated risks in vulnerable populations, and warrant further clinical investigation.
Subject	Medicine, Health and Life Sciences; Bowel preparation; IBD mouse model; gut microbiota; Pathogen colonization; Osmolality; Salmonella enterica serovat Typhimurium; Polyethylene glycol (PEG); Inflammatory Bowel Disease (IBD); Pathobionts; Translocation; humanized mice; Gut-on-a-chip; DSS
Type	Dataset
Date Available	2024-12-12
Provider	University of British Columbia Library
License	CC-BY 4.0
DOI	10.14288/1.0450342
URI	https://doi.org/10.5683/SP3/DGHLV4
Publisher DOI	https://doi.org/10.5683/SP3/DGHLV4
Rights URI	http://creativecommons.org/licenses/by/4.0
Aggregated Source Repository	Dataverse

Item Media

Item Citations and Data

License

CC-BY 4.0