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Lipopolysaccharide differentially alters systemic and brain glucocorticoid levels in neonatal and adult mice Hamden, Jordan; Salehzadeh, Melody; Bajaj, Hitasha; Li, Michael; Soma, Kiran

Description

We treated male and female C57BL/6J mice at postnatal day 5 (PND5) or PND90 with lipopolysaccharide (LPS; 50 µg/kg bw i.p.) or vehicle and collected blood and brain after 4 hr. We microdissected the prefrontal cortex, hippocampus, hypothalamus, and amygdala. We measured 7 steroids, including corticosterone, via liquid chromatography-tandem mass spectrometry and measured transcripts for key steroidogenic enzymes and hypothalamic-pituitary-adrenal axis components (Cyp11b1, Hsd11b1, Hsd11b2, Crh, Crhr1, Pomc) via qPCR.

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Title	Lipopolysaccharide differentially alters systemic and brain glucocorticoid levels in neonatal and adult mice
Creator	Hamden, Jordan; Salehzadeh, Melody; Bajaj, Hitasha; Li, Michael; Soma, Kiran
Contributor	Hamden, Jordan
Date Issued	2024-11-18
Description	We treated male and female C57BL/6J mice at postnatal day 5 (PND5) or PND90 with lipopolysaccharide (LPS; 50 µg/kg bw i.p.) or vehicle and collected blood and brain after 4 hr. We microdissected the prefrontal cortex, hippocampus, hypothalamus, and amygdala. We measured 7 steroids, including corticosterone, via liquid chromatography-tandem mass spectrometry and measured transcripts for key steroidogenic enzymes and hypothalamic-pituitary-adrenal axis components (Cyp11b1, Hsd11b1, Hsd11b2, Crh, Crhr1, Pomc) via qPCR.
Subject	Medicine, Health and Life Sciences; Stress; Lipopolysaccharide; Glucocorticoid; Immune and Nervous system
Type	Dataset
Date Available	2024-08-16
Provider	University of British Columbia Library
License	CC-BY 4.0
DOI	10.14288/1.0447283
URI	https://doi.org/10.5683/SP3/QMTF0K
Publisher DOI	https://doi.org/10.5683/SP3/QMTF0K
Rights URI	http://creativecommons.org/licenses/by/4.0
Aggregated Source Repository	Dataverse

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CC-BY 4.0