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Decoupling of height growth and drought or pest resistance tradeoffs is revealed through multiple common-garden experiments of lodgepole pine Liu, Yang; Erbilgin, Nadir; Cappa, Eduardo Pablo; Chen, Charles; Ratcliffe, Blaise; Wei, Xiaojing; Klutsch, Jennifer G.; Ullah, Aziz; Azcona, Jaime Sebastian; Thomas, Barb R.; El-Kassaby, Yousry A.
Description
We selected five lodgepole pine populations, representing a total of 224 maternal half-sib families, grown in four progeny test sites (> 35 yrs) located along various climatic gradients in central Alberta, Canada. Across the four progeny test sites, we chose a total of 1,490 trees for phenotyping. Height growth (m) was measured at age 35-yr with a clinometer. Carbon isotope ratio (δ13C, in ‰) analysis was performed at Alberta Innovates in Victoria, using outside slabs cut and ground from the 5 mm increment cores taken from the north side of each tree at approximately breast height (1.3 m) at age 35. Samples were analyzed using an established method on a MAT253 Mass Spectrometer with Conflo IV interface and a Fisons NA1500 EA. We assessed the severity of WGR infection in the test sites by a qualitative scoring system with discrete categories ranging from no gall symptoms to deceased (four tiers) for all trees sampled at age 36-yr. We also investigated these trees’ suitability to MPB. Host tree suitability to the beetles was evaluated by quantifying defense chemicals (mainly monoterpenes) using a Gas Chromatography/Flame Ionization Detector based on cambial tissues collected by a hole punch when trees were actively growing, coinciding with MPB flight in western Canada. Then, chemical profiling was performed to test against MPB performance based on laboratory bioassays. A cutoff of four categories was used to classify trees with different MPB suitability levels. In addition, we performed dendrochronological analysis to calculate drought resistance indices based on changes in tree-ring width before and after drought event.
Item Metadata
| Title |
Decoupling of height growth and drought or pest resistance tradeoffs is revealed through multiple common-garden experiments of lodgepole pine
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| Creator | |
| Contributor | |
| Date Issued |
2022-12-28
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| Description |
We selected five lodgepole pine populations, representing a total of 224 maternal half-sib families, grown in four progeny test sites (> 35 yrs) located along various climatic gradients in central Alberta, Canada. Across the four progeny test sites, we chose a total of 1,490 trees for phenotyping. Height growth (m) was measured at age 35-yr with a clinometer. Carbon isotope ratio (δ13C, in ‰) analysis was performed at Alberta Innovates in Victoria, using outside slabs cut and ground from the 5 mm increment cores taken from the north side of each tree at approximately breast height (1.3 m) at age 35. Samples were analyzed using an established method on a MAT253 Mass Spectrometer with Conflo IV interface and a Fisons NA1500 EA. We assessed the severity of WGR infection in the test sites by a qualitative scoring system with discrete categories ranging from no gall symptoms to deceased (four tiers) for all trees sampled at age 36-yr. We also investigated these trees’ suitability to MPB. Host tree suitability to the beetles was evaluated by quantifying defense chemicals (mainly monoterpenes) using a Gas Chromatography/Flame Ionization Detector based on cambial tissues collected by a hole punch when trees were actively growing, coinciding with MPB flight in western Canada. Then, chemical profiling was performed to test against MPB performance based on laboratory bioassays. A cutoff of four categories was used to classify trees with different MPB suitability levels. In addition, we performed dendrochronological analysis to calculate drought resistance indices based on changes in tree-ring width before and after drought event.
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| Subject |
Earth and Environmental Sciences; Medicine, Health and Life Sciences; Earth and Environmental Sciences; Medicine, Health and Life Sciences; climate change; common-garden approach; functional tradeoff; trait coordination; non-adaptive evolutionary process; resource allocation; Earth and Environmental Sciences; Medicine, Health and Life Sciences; climate change; common-garden approach; functional tradeoff; trait coordination; non-adaptive evolutionary process; resource allocation
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| Geographic Location | |
| Type | |
| Date Available |
2022-11-05
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| Provider |
University of British Columbia Library
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| License |
CC0 Waiver
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| DOI |
10.14288/1.0422915
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| URI | |
| Publisher DOI | |
| Rights URI | |
| Country |
Canada
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| Aggregated Source Repository |
Dataverse
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Licence
CC0 Waiver