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Madagascar Hissing Cockroach hemolymph PO2, PCO2, and gas exchange parameters Matthews, Philip; Rowe, Tormod; Gutbrod, Martin

Description

Abstract

This data set contains in vivo measurements of hemolymph PO2 and PCO2 made on Madagascar hissing cockroaches (Gromphadorhina portentosa) using implanted micro fiber optic sensors (optodes). In addition, rate of CO2 release, ventilation frequency, and durations of the tri-phasic discontinuous gas exchange cycle associated with these measurements are also recorded. All measurements were performed while the cockroaches were breathing either normoxic and normocapnic air, hypoxic air (10 kPa PO2), or hypercapnic air (2 kPa PCO2) at 22 ºC.; Methods

These data were collected and analyzed according to the materials and methods in the associated JEB article. Briefly, Madagascar hissing cockroaches (Gromphadorhina portentosa) were secured in a respirometry chamber with PCO2 and PO2 optodes implanted in their hemocoel. A reflection sensor beneath their abdomen recorded abdominal pumping movements. Cockroaches were exposed to normoxic normocapnia, hypoxic normocapnia, and normoxic hypercapnia. Roaches were exposed to these gas mixtures while intact and breathing continuously, and then decapitated which induced discontinuous gas exchange cycles (DGCs), before being exposed to the same gas mixtures as before. During these exposures, hemolymph PO2, PCO2, VCO2, and abdominal movements were recorded continuously. The summary data extracted form these data traces are presented here.; Usage notes

Each treatment assumed 6 cockroaches were used. Empty cells indicate that either equipment failure resulted in a parameter (e.g. hemolymph PCO2) not being recorded from that individual insect, or the insect did not display the breathing pattern required to record a certain parameter (e.g., the cockroach began breathing discontinuously, preventing the recording of continuous gas exchange parameters).

Item Metadata

Title	Madagascar Hissing Cockroach hemolymph PO2, PCO2, and gas exchange parameters
Creator	Matthews, Philip; Rowe, Tormod; Gutbrod, Martin
Date Issued	2022-02-02
Description	<b>Abstract</b><br/> This data set contains <em>in vivo </em>measurements of<em> </em>hemolymph PO<sub>2</sub> and PCO<sub>2</sub> made on Madagascar hissing cockroaches (<em>Gromphadorhina portentosa</em>) using implanted micro fiber optic sensors (optodes). In addition, rate of CO<sub>2</sub> release, ventilation frequency, and durations of the tri-phasic discontinuous gas exchange cycle associated with these measurements are also recorded. All measurements were performed while the cockroaches were breathing either normoxic and normocapnic air, hypoxic air (10 kPa PO<sub>2</sub>), or hypercapnic air (2 kPa PCO<sub>2</sub>) at 22 ºC.</p>; <b>Methods</b><br /> These data were collected and analyzed according to the materials and methods in the associated JEB article. Briefly, Madagascar hissing cockroaches (<em>Gromphadorhina portentosa</em>) were secured in a respirometry chamber with PCO<sub>2</sub> and PO<sub>2</sub> optodes implanted in their hemocoel. A reflection sensor beneath their abdomen recorded abdominal pumping movements. Cockroaches were exposed to normoxic normocapnia, hypoxic normocapnia, and normoxic hypercapnia. Roaches were exposed to these gas mixtures while intact and breathing continuously, and then decapitated which induced discontinuous gas exchange cycles (DGCs), before being exposed to the same gas mixtures as before. During these exposures, hemolymph PO<sub>2</sub>, PCO<sub>2</sub>, VCO<sub>2</sub>, and abdominal movements were recorded continuously. The summary data extracted form these data traces are presented here.</p>; <b>Usage notes</b><br /> Each treatment assumed 6 cockroaches were used. Empty cells indicate that either equipment failure resulted in a parameter (e.g. hemolymph PCO2) not being recorded from that individual insect, or the insect did not display the breathing pattern required to record a certain parameter (e.g., the cockroach began breathing discontinuously, preventing the recording of continuous gas exchange parameters).</p>
Subject	Other; Other; Other
Type	Dataset
Notes	Dryad version number: 7</p> Version status: submitted</p> Dryad curation status: Published</p> Sharing link: https://datadryad.org/stash/share/AkBA5YgEVB5TyDnytQHo67LCr3XCXxVq_YPpyx8wpvA</p> Storage size: 850156</p> Visibility: public</p>
Date Available	2022-01-25
Provider	University of British Columbia Library
License	CC0 Waiver
DOI	10.14288/1.0406462
URI	https://doi.org/10.5683/SP3/FD3RZP
Publisher DOI	https://doi.org/10.5683/SP3/FD3RZP
Grant Funding Agency	Natural Sciences and Engineering Research Council of Canada; Natural Sciences and Engineering Research Council of Canada; Natural Sciences and Engineering Research Council of Canada
Rights URI	https://creativecommons.org/publicdomain/zero/1.0/
Aggregated Source Repository	Dataverse

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