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Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera Feau, Nicolas; Wong, Barbara; Hamelin, Richard

Description

Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.

Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA.

Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).

Item Metadata

Title	Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera
Creator	Feau, Nicolas; Wong, Barbara; Hamelin, Richard
Date Issued	2021-05-20
Description	Abstract Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens. ; Methods Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA. Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).
Subject	Other; RNA and DNA extraction; cDNA synthesis; qPCR; RT-qPCR; Phytophthora ramorum; Grosmannia clavigera; tree pathogen; QuantiTect Reverse Transcription Kit (QIAGEN)
Type	Dataset
Notes	Dryad version number: 2 Version status: submitted Dryad curation status: Published Sharing link: https://datadryad.org/stash/share/0aNNOOZ1MhDyvvih6bARA7n35F7br_OYXXy-nj8iXHQ</p> Storage size: 40927 Visibility: public
Date Available	2020-01-07
Provider	University of British Columbia Library
License	CC0 1.0
DOI	10.14288/1.0398059
URI	https://doi.org/10.5683/SP2/ZSAWXJ
Publisher DOI	https://doi.org/10.5683/SP2/ZSAWXJ; https://doi.org/10.5061/dryad.jq2bvq861
Rights URI	http://creativecommons.org/publicdomain/zero/1.0
Aggregated Source Repository	Dataverse

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