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Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera Feau, Nicolas; Wong, Barbara; Hamelin, Richard

Description

Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.


Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA.

Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).



Item Metadata

Title
Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera
Creator
Feau, Nicolas; Wong, Barbara; Hamelin, Richard
Date Issued
2021-05-20
Description
Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.

; Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA.

Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).
Subject
Other; RNA and DNA extraction; cDNA synthesis; qPCR; RT-qPCR; Phytophthora ramorum; Grosmannia clavigera; tree pathogen; QuantiTect Reverse Transcription Kit (QIAGEN)
Type
Dataset
Notes

Dryad version number: 2

Version status: submitted

Dryad curation status: Published

Sharing link: https://datadryad.org/stash/share/0aNNOOZ1MhDyvvih6bARA7n35F7br_OYXXy-nj8iXHQ</p>

Storage size: 40927

Visibility: public
Date Available
2020-01-07
Provider
University of British Columbia Library
License
CC0 1.0
DOI
10.14288/1.0398059
URI
https://doi.org/10.5683/SP2/ZSAWXJ
Publisher DOI
https://doi.org/10.5683/SP2/ZSAWXJ; https://doi.org/10.5061/dryad.jq2bvq861
Rights URI
http://creativecommons.org/publicdomain/zero/1.0
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Dataverse

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CC0 1.0