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Veltjen, Emily; Asselman, Pieter; Hernández Rodríguez, Majela; Palmarola Bejerano, Alejandro; Testé Lozano, Ernesto; González Torres, Luis Roberto; Goetghebeur, Paul; Larridon, Isabel; Samain, Marie-Stéphanie

<b>Abstract</b><br/>Conserving tree populations safeguards forests since they represent key elements of the ecosystem. The genetic characteristics underlying the evolutionary success of the tree growth form: high genetic diversity, extensive gene flow and strong species integrity, contribute to their survival in terms of adaptability. However, different biological and landscape contexts challenge these characteristics. This study employs 63 de novo developed microsatellite or SSR (Single Sequence Repeat) markers in different datasets of nine Neotropical Magnolia species. The genetic patterns of these protogynous, insect-pollinated tree species occurring in fragmented, highly-disturbed landscapes were investigated. Datasets containing a total of 340 individuals were tested for their genetic structure and degree of inbreeding. Analyses for genetic structure depicted structuring between species, i.e. strong species integrity. Within the species, all but one population pair were considered moderate to highly differentiated, i.e. no indication of extensive gene flow between populations. No overall correlation was observed between genetic and geographic distance of the pairwise species’ populations. In contrast to the pronounced genetic structure, there was no evidence of inbreeding within the populations, suggesting mechanisms favouring cross pollination and/or selection for more genetically diverse, heterozygous offspring. In conclusion, the data illustrate that the Neotropical Magnolias in the context of a fragmented landscape still have ample gene flow within populations, yet little gene flow between populations.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_acunae32_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 20 individuals of 1 population of Magnolia cubensis subspecies acunae. DNA of the 20 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the Cuban forest. The 20 individuals were genotyped using 32 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_acunae32_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_cubensis31_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 20 individuals of 1 population of Magnolia cubensis subspecies cubensis. DNA of the 20 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the Cuban forest. The 20 individuals were genotyped using 31 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_cubensis31_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_dodecapetala21_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia dodecapetala: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forest of the Lesser Antilles. The 40 individuals were genotyped using 21 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_dodecapetala21_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_domingensis23_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia domingensis: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forest of the Dominican Republic. The 40 individuals were genotyped using 23 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_domingensis23_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_hamorii24_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia hamorii: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forest of the Dominican Republic. The 40 individuals were genotyped using 24 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_hamorii24_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_ekmanii31_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia ekmanii: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forest of Haiti. The 40 individuals were genotyped using 31 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_ekmanii31_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_lacandonica23_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia lacandonica: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forests of Mexico. The 40 individuals were genotyped using 23 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_lacandonica23_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_pallescens20_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia pallescens: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forests of the Dominican Republic. The 40 individuals were genotyped using 20 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_pallescens20_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_portoricensis29_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 40 individuals of 2 populations of Magnolia portoricensis: 20 individuals per population. DNA of the 40 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forests of Puerto Rico. The 40 individuals were genotyped using 29 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_portoricensis29_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_splendens25_CONVERT</h4><div class="o-metadata__file-description">This file includes SSR/microsatellite genotype data of 20 individuals of 1 population of Magnolia splendens. DNA of the 20 individuals was extracted from leaves using the CTAB method. The leaves were dried on silica-gel and collected from trees in the forests of Puerto Rico. The 20 individuals were genotyped using 25 de novo developed microsatellites.</div><div class="o-metadata__file-name">Dataset_splendens25_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_dataset1_63_CONVERT</h4><div class="o-metadata__file-description">Dataset 1 comprises 340 individuals representing 17 populations of Neotropical Magnolias, genotyped for all 63 microsatellite markers where possible, including the assumed monomorphic data (See Supplementary Table S2: categories A, B and C; Veltjen et al. 2018).</div><div class="o-metadata__file-name">Dataset1a_63_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_dataset2_63_CONVERT</h4><div class="o-metadata__file-description">Dataset 2 comprises 340 individuals representing 17 populations of Neotropical Magnolias, genotyped for 63 microsatellite markers where possible, excluding the assumed monomorphic data (See Supplementary Table S2: categories A and B; Veltjen et al. 2018).</div><div class="o-metadata__file-name">Dataset2a_63_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">MAGNOLIA_SSR_dataset3_10_CONVERT</h4><div class="o-metadata__file-description">Dataset 3 comprises 260 individuals representing 13 populations of the 8 Magnolia taxa of the section Talauma subsection Splendentes (See Veltjen et al. 2018 Table 1: Class. = TAS), genotyped for 10 microsatellite markers (See Supplementary Table S2: marker names indicated with an asterisk; Veltjen et al. 2018).</div><div class="o-metadata__file-name">Dataset3a_10_CONVERT.xlsx</br></div><div class="o-metadata__file-name"></div></div>

Cuba; Haiti; Puerto Rico; Guadeloupe; Hispaniola; Dominican Republic; West-Indies; Martinique; Mexico; Lesser Antilles; Neotropics; Caribbean

Dryad version number: 1</p>

Version status: submitted</p>

Dryad curation status: Published</p>

Sharing link: https://datadryad.org/stash/share/N_DHoZDvgZRpuhH2iw6KR8Z5EkyDCqmkiXTGPKZ2vaw</p>

Storage size: 715361</p>

Visibility: public</p>

University of British Columbia Library

10.14288/1.0398058

https://doi.org/10.5683/SP2/ZS0VLW; https://doi.org/10.5061/dryad.0m625h4

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