Open Collections

Brar, Simren; Tsui, Clement K. M.; Dhillon, Braham; Bergeron, Marie-Josée; Joly, David L.; Zambino, P. J.; El-Kassaby, Yousry A.; Hamelin, Richard C.

<b>Abstract</b><br/>White pine blister rust is caused by the fungal pathogen Cronartium ribicola J.C. Fisch (Basidiomycota, Pucciniales). This invasive alien pathogen was introduced into North America at the beginning of the 20th century on pine seedlings imported from Europe and has caused serious economic and ecological impacts. In this study, we applied a population and landscape genetics approach to understand the patterns of introduction and colonization as well as population structure and migration of C. ribicola. We characterized 1,292 samples of C. ribicola from 66 geographic locations in North America using single nucleotide polymorphisms (SNPs) and evaluated the effect of landscape features, host distribution, and colonization history on the structure of these pathogen populations. We identified eastern and western genetic populations in North America that are strongly differentiated. Genetic diversity is two to five times higher in eastern populations than in western ones, which can be explained by the repeated accidental introductions of the pathogen into northeastern North America compared with a single documented introduction into western North America. These distinct genetic populations are maintained by a barrier to gene flow that corresponds to a region where host connectivity is interrupted. Furthermore, additional cryptic spatial differentiation was identified in western populations. This differentiation corresponds to landscape features, such as mountain ranges, and also to host connectivity. We also detected genetic differentiation between the pathogen populations in natural stands and plantations, an indication that anthropogenic movement of this pathogen still takes place. These results highlight the importance of monitoring this invasive alien tree pathogen to prevent admixture of eastern and western populations where different pathogen races occur.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Cronartium ribicola SNP genalex SNPs</h4><div class="o-metadata__file-description">An excel spreadsheet containing genotypes of 1292 Cronartium ribicola individuals genotyped at 31 SNPs using sequenom. The data are arranged for processing in Genalex http://biology-assets.anu.edu.au/GenAlEx/Welcome.html</div><div class="o-metadata__file-name">Dryad_Genalex.xlsx</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Cronartium ribicola Arlequin</h4><div class="o-metadata__file-description">Cronartium ribicola Arlequin file comprising genotypes at 31 SNP loci for 1292 individuals sampled across 66 populations in North America and separated into eastern and western regions for AMOVA analyses</div><div class="o-metadata__file-name">CR_1292_region.arp</br></div></div>

North America

Dryad version number: 1</p>

Version status: submitted</p>

Dryad curation status: Published</p>

Sharing link: https://datadryad.org/stash/share/oyY3emqX6SSp-URM8500k9t57ZK0wdx_pYLLoI37Zl4</p>

Storage size: 635922</p>

Visibility: public</p>

University of British Columbia Library

10.14288/1.0398013

https://doi.org/10.5683/SP2/XZYUZH; https://doi.org/10.5061/dryad.962dt

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