UBC Research Data

Data from: Divergent transcriptional patterns are related to differences in hypoxia tolerance between the intertidal and the subtidal sculpins Mandic, Milica; Ramon, Marina L.; Gracey, Andrew Y.; Richards, Jeffrey G.


Transcriptionally mediated phenotypic plasticity as a mechanism of modifying traits in response to an environmental challenge remains an important area of study. We compared the transcriptional responses to low-oxygen (hypoxia) of the hypoxia tolerant intertidal fish, the tidepool sculpin (Oligocottus maculosus) with the closely related hypoxia intolerant subtidal fish, the silverspotted sculpin (Blepsias cirrhosus) to determine if these species use different mechanisms to cope with hypoxia. Individuals from each species were exposed to environmental O2 tensions chosen to yield a similar level of tissue hypoxia and gene transcription was assessed in the liver over time. There was an effect of time in hypoxia, where the greatest transcriptional change in the silverspotted sculpin occurred between 3 to 24 hours in contrast to the tidepool sculpin where the largest transcriptional change occurred between 24 and 72 hours of hypoxia. A number of genes showed similar hypoxia-induced transcription patterns in both species (e.g. genes associated with glycolysis and apoptosis) suggesting they are involved in a conserved hypoxia response. A large set of genes showed divergent transcriptional patterns in the two species, including fatty acid oxidation and oxidative phosphorylation, suggesting that these biological processes may contribute to explaining variation in hypoxia tolerance in these species. When both species were exposed to a single environmental O2 tension, large transcriptional responses were seen in the hypoxia intolerant silverspotted sculpin while almost no response was observed in the hypoxia tolerant tidepool sculpin. Overall, divergent transcription patterns in response to both magnitude and duration of hypoxia provide insights into the processes that may determine an animal's capacity to tolerate frequent bouts of hypoxia in the wild.; Usage notes
Transcriptomic data for O. maculosus and B. cirrhosisRaw data of hybridization assays for both species of sculpin for all time-points at all O2 tensions. Available in ArrayExpress at http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3043/. When reusing these data, please include the following citation: Microarray data are available in the ArrayExpress database (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-3043.Microarray Analysis of VarianceThe R script containing the code for microarray analysis of variance used in the r/maanova package.MaanovaAnalysis.rtf
Principal Component AnalysisThe R script containing the code for principal component analysis via the 'Easy Microarray data Analysis' package.PrincipalComponentAnalysis.R
Tidepool Sculpin Data FileTidepool sculpin coalesced data of hybridization assays.TidepoolSculpinDataFile.txt
Tidepool Sculpin Design FileTidepool sculpin design data file for running r/maanova.TidepoolSculpinDesignFile.txt
Silverspotted Sculpin Data FileSilverspotted sculpin coalesced data of hybridization assays.SilverspottedSculpinDataFile.txt
Silverspotted Sculpin Design FileSilverspotted sculpin design data file for running r/maanova.SilverspottedSculpinDesignFile.txt

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