UBC Research Data

Data from: Acid secretion by the boring organ of the burrowing giant clam, Tridacna crocea Hill, Richard W.; Armstrong, Eric J.; Inaba, Kazuo; Morita, Masaya; Tresguerres, Martin; Stillman, Jonathon H.; Roa, Jinae N.; Kwan, Garfield T.

Description

Abstract
The giant clam Tridacna crocea, native to Indo-Pacific coral reefs, is noted for its unique ability to bore fully into coral rock and is a major agent of reef bioerosion. However, T. crocea’s mechanism of boring has remained a mystery despite decades of research. By exploiting a new, two-dimensional pH-sensing technology and manipulating clams to press their presumptive boring tissue (the pedal mantle) against pH-sensing foils, we show that this tissue lowers the pH of surfaces it contacts by more than 2 pH units below seawater pH day and night. Acid secretion is likely mediated by vacuolar-type H+-ATPase, which we demonstrate (by immunofluorescence) is abundant in the pedal mantle outer epithelium. Our discovery of acid secretion solves this decades-old mystery and reveals that, during bioerosion, T. crocea can liberate reef constituents directly to the soluble phase, rather than producing sediment alone as earlier assumed.; Usage notes
ME_ST_XX_00001Individual ID: Clam 1 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00006Individual ID: Clam 5 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00011Individual ID: Clam 3 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00013Individual ID: Clam 6 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00018Individual ID: Clam 7 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00021Individual ID: Clam 8 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00026Individual ID: Clam 1 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00034Individual ID: Clam 5 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00040Individual ID: Clam 3 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00042Individual ID: Clam 6 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00044Individual ID: Clam 7 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00049Individual ID: Clam 8 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00031Individual ID: Clam 4 (Day) ; Time: 10 March 2017 14:40ME_ST_XX_00076Individual ID: Clam 4 (Night) ; Time: 10 March 2017 20:45ME_ST_XX_00059Individual ID: Clam 2 (Day) ; Time: 11 March 2017 14:06ME_ST_XX_00061Individual ID: Clam 9 (Day) ; Time: 11 March 2017 14:06ME_ST_XX_00083Individual ID: Clam 9 (Night) ; Time: 11 March 2017 22:24ME_ST_XX_00088Individual ID: Clam 2 (Day) ; Time: 11 March 2017 14:06

Item Media

Item Citations and Data

Licence

CC0 Waiver

Usage Statistics