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Data from: The molecular phylogeny of Chionaster nivalis reveals a novel order of psychrophilic and globally distributed Tremellomycetes (Fungi, Basidiomycota) Irwin, Nicholas; Twynstra, Chantelle; Mathur, Varsha; Keeling, Patrick
Description
<b>Abstract</b><br/><p style="margin-bottom:11px;">Snow and ice present challenging substrates for cellular growth, yet microbial snow communities not only exist, but are diverse and ecologically impactful. These communities are dominated by green algae, but additional organisms, such as fungi, are also abundant and may be important for nutrient cycling, syntrophic interactions, and community structure in general. However, little is known about these non-algal community members, including their taxonomic affiliations. An example of this is <i>Chionaster nivalis</i>, a unicellular fungus that is morphologically enigmatic and frequently observed in snow communities globally. Despite being described over one hundred years ago, the phylogeny and higher-level taxonomic classifications of <i>C. nivalis</i> remain unknown. Here, we isolated and sequenced the internal transcribed spacer (ITS) and the D1-D2 region of the large subunit ribosomal RNA gene of <i>C. nivalis</i>,<i> </i>providing a molecular barcode for future studies. Phylogenetic analyses using the ITS and D1-D2 region revealed that <i>C. nivalis</i> is part of a novel lineage in the class Tremelomycetes (Basidiomycota, Agaricomycotina) for which a new order, Chionasterales ord. nov. (MB838717), and family, Chionasteraceae fam. nov. (MB838718), are proposed. Comparisons between <i>C. nivalis</i> and sequences generated from environmental surveys revealed that the Chionasterales are globally distributed and probably psychrophilic, as they appear to be limited to the high alpine and arctic regions. These results highlight the unexplored diversity that exists within these extreme habitats and emphasize the utility of single-cell approaches in characterizing these complex algal-dominated communities.</p>; <b>Usage notes</b><br />
Included are the internal transcribed spacer (ITS) and D1-D2 (LSU) sequences for <em>Chionaster nivalis </em>as well as the alignments and phylogenies presented in the mansucript. Data files have been organized based on which figures they were used in.</p>
Item Metadata
| Title |
Data from: The molecular phylogeny of Chionaster nivalis reveals a novel order of psychrophilic and globally distributed Tremellomycetes (Fungi, Basidiomycota)
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| Creator | |
| Date Issued |
2021-05-20
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| Description |
<b>Abstract</b><br/><p style="margin-bottom:11px;">Snow and ice present challenging substrates for cellular growth, yet microbial snow communities not only exist, but are diverse and ecologically impactful. These communities are dominated by green algae, but additional organisms, such as fungi, are also abundant and may be important for nutrient cycling, syntrophic interactions, and community structure in general. However, little is known about these non-algal community members, including their taxonomic affiliations. An example of this is <i>Chionaster nivalis</i>, a unicellular fungus that is morphologically enigmatic and frequently observed in snow communities globally. Despite being described over one hundred years ago, the phylogeny and higher-level taxonomic classifications of <i>C. nivalis</i> remain unknown. Here, we isolated and sequenced the internal transcribed spacer (ITS) and the D1-D2 region of the large subunit ribosomal RNA gene of <i>C. nivalis</i>,<i> </i>providing a molecular barcode for future studies. Phylogenetic analyses using the ITS and D1-D2 region revealed that <i>C. nivalis</i> is part of a novel lineage in the class Tremelomycetes (Basidiomycota, Agaricomycotina) for which a new order, Chionasterales ord. nov. (MB838717), and family, Chionasteraceae fam. nov. (MB838718), are proposed. Comparisons between <i>C. nivalis</i> and sequences generated from environmental surveys revealed that the Chionasterales are globally distributed and probably psychrophilic, as they appear to be limited to the high alpine and arctic regions. These results highlight the unexplored diversity that exists within these extreme habitats and emphasize the utility of single-cell approaches in characterizing these complex algal-dominated communities.</p>; <b>Usage notes</b><br />
Included are the internal transcribed spacer (ITS) and D1-D2 (LSU) sequences for <em>Chionaster nivalis </em>as well as the alignments and phylogenies presented in the mansucript. Data files have been organized based on which figures they were used in.</p> |
| Subject | |
| Type | |
| Notes |
Dryad version number: 6</p> Version status: submitted</p> Dryad curation status: Published</p> Sharing link: https://datadryad.org/stash/share/_HkzB-5PI5Sqlb7KkZmpqxpj7nbzHscbqNI791SWGXE</p> Storage size: 775982</p> Visibility: public</p> |
| Date Available |
2021-03-01
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| Provider |
University of British Columbia Library
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| License |
CC0 1.0
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| DOI |
10.14288/1.0397878
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| URI | |
| Publisher DOI | |
| Grant Funding Agency |
Gordon and Betty Moore Foundation
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| Rights URI | |
| Aggregated Source Repository |
Dataverse
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CC0 1.0