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Data from: EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling Loucks, Catrina M.; Park, Kwangjin; Walker, Denise S.; McEwan, Andrea H.; Timbers, Tiffany A.; Ardiel, Evan L.; Grundy, Laura J.; Li, Chunmei; Johnson, Jacque-Lynne; Kennedy, Julie; et al.
Description
<b>Abstract</b><br/>Neurons throughout the mammalian brain possess non-motile cilia, organelles with varied functions in sensory physiology and cellular signaling, yet their roles in these neurons are poorly understood. To shed light into their functions, we studied EFHC1, an evolutionarily conserved protein required for motile cilia function and linked to a common form of inherited epilepsy in humans, juvenile myoclonic epilepsy (JME). We demonstrate that C. elegans EFHC1 functions within specialized non-motile mechanosensory cilia, where it regulates neuronal activation and dopamine signaling. EFHC1 also localizes at the synapse, where it further modulates dopamine signaling in cooperation with the orthologue of an R-type voltage-gated calcium channel. Our findings unveil a previously undescribed dual-regulation of neuronal excitability at sites of neuronal sensory input (cilium) and neuronal output (synapse). Such a distributed regulatory mechanism may be essential for establishing neuronal activation thresholds under physiological conditions, and when impaired, may represent a novel pathomechanism for epilepsy.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">EFHC1-data_2019-02-04</h4><div class="o-metadata__file-description">The zip file contains all the data used to generate the figures in the associated manuscript. It is organized with folders containing different data types (from different kinds of experiments). It is further divided into folders containing data used to generate specific figures.</div><div class="o-metadata__file-name"></div></div>
Item Metadata
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Data from: EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling
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| Date Issued |
2021-05-19
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| Description |
<b>Abstract</b><br/>Neurons throughout the mammalian brain possess non-motile cilia, organelles with varied functions in sensory physiology and cellular signaling, yet their roles in these neurons are poorly understood. To shed light into their functions, we studied EFHC1, an evolutionarily conserved protein required for motile cilia function and linked to a common form of inherited epilepsy in humans, juvenile myoclonic epilepsy (JME). We demonstrate that C. elegans EFHC1 functions within specialized non-motile mechanosensory cilia, where it regulates neuronal activation and dopamine signaling. EFHC1 also localizes at the synapse, where it further modulates dopamine signaling in cooperation with the orthologue of an R-type voltage-gated calcium channel. Our findings unveil a previously undescribed dual-regulation of neuronal excitability at sites of neuronal sensory input (cilium) and neuronal output (synapse). Such a distributed regulatory mechanism may be essential for establishing neuronal activation thresholds under physiological conditions, and when impaired, may represent a novel pathomechanism for epilepsy.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">EFHC1-data_2019-02-04</h4><div class="o-metadata__file-description">The zip file contains all the data used to generate the figures in the associated manuscript. It is organized with folders containing different data types (from different kinds of experiments). It is further divided into folders containing data used to generate specific figures.</div><div class="o-metadata__file-name"></div></div>
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| Notes |
Dryad version number: 1</p> Version status: submitted</p> Dryad curation status: Published</p> Sharing link: https://datadryad.org/stash/share/ZGy8EkCEDZgqqRJWL2cZOKGq3Qf2pUzanilhKUK73HQ</p> Storage size: 81790293</p> Visibility: public</p> |
| Date Available |
2020-06-24
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| Provider |
University of British Columbia Library
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| License |
This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver
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| DOI |
10.14288/1.0397781
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| Aggregated Source Repository |
Dataverse
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Licence
This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver