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<strong>Background</strong>: Studying how trophic traits and niche use are related in natural populations is important in order to understand adaptation and specialization. Here, we describe trophic trait diversity in twenty-five Norwegian freshwater threespine stickleback populations and their putative marine ancestor, and relate trait differences to postglacial lake age. By studying lakes of different ages, depths and distance to the sea we examine key environmental variables that may predict adaptation in trophic position and habitat use. We measured trophic traits including geometric landmarks that integrated variation in head shape as well as gillraker length and number. Trophic position (T_pos) and niche use (α) were estimated from stable isotopes (δ¹³C, δ¹⁵N). A comparison of head shape was also made with two North American benthic-limnetic species pairs. Results: We found that head shape differed between marine and freshwater sticklebacks, with marine sticklebacks having more upturned mouths, smaller eyes, larger opercula and deeper heads. Size-adjusted gillraker lengths were larger in marine than in freshwater stickleback. Norwegian sticklebacks were compared on the same head shape axis as the one differentiating the benthic-limnetic North American threespine stickleback species pairs. Here, Norwegian freshwater sticklebacks with a more "limnetic head shape" had more and longer gillrakers than sticklebacks with "benthic head shape". The "limnetic morph" was positively associated with deeper lakes. Populations differed in α (mean ± sd: 0.76 ± 0.29) and T_pos (3.47 ± 0.27), where α increased with gillraker length. Larger fish had a higher Tpos than smaller fish. Compared to the ecologically divergent stickleback species pairs and solitary lake populations in North America, Norwegian freshwater sticklebacks had similar range in T_pos and α values, but much less trait divergences. Conclusions: Our results showed trait divergences between threespine stickleback in marine and freshwater environments. Freshwater populations diverged in trophic ecology and trophic traits, but trophic ecology was not related to the elapsed time in freshwater. Norwegian sticklebacks used the same niches as the ecologically divergent North American stickleback species pairs. However, as trophic trait divergences were smaller, and not strongly associated with the ecological niche, ecological adaptations along the benthic-limnetic axis were less developed in Norwegian sticklebacks.</p>; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Read me file with location codes</h4><div class="o-metadata__file-name">0 Sample codes to locality names.xls</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">1 Dataset-Freshwater</h4><div class="o-metadata__file-description">Data comparing the freshwater stickleback only; with principal component scores (see details in the manuscript).</div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">2 Dataset-Freshwater and marine</h4><div class="o-metadata__file-description">Data comparing the marine and freshwater stickleback; with principal component scores (see details in the manuscript).</div><div class="o-metadata__file-name"></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">3 Dataset- Freshwater, marine and North American</h4><div class="o-metadata__file-description">Data comparing the marine and freshwater stickleback from Norway and the two North American species pairs; with principal component scores (see details in the manuscript).</div><div class="o-metadata__file-name"></div></div>

Dryad version number: 3</p>

Version status: submitted</p>

Dryad curation status: Published</p>

Sharing link: https://datadryad.org/stash/share/9q94sSHI6ex5_A1xM3COoSKiaXhSDwtrXhQ0L3IPrFE</p>

Storage size: 664996</p>

Visibility: public</p>