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Data from: Phylogeny, evidence for a cryptic plastid, and distribution of Chytriodinium parasites (Dinophyceae) infecting copepods Strassert, Jürgen F.H.; Hehenberger, Elisabeth; del Campo, Javier; Okamoto, Noriko; Kolisko, Martin; Richards, Thomas A.; Worden, Alexandra Z.; Santoro, Alyson E.; Keeling, Patrick J.; Campo, Javier
Description
<b>Abstract</b><br/>Spores of the dinoflagellate Chytriodinium are known to infest copepod eggs causing their lethality. Despite the potential to control the population of such an ecologically important host, knowledge about Chytriodinium parasites is limited: we know little about phylogeny, parasitism, abundance, or geographical distribution. We carried out genome sequence surveys on four manually isolated sporocytes from the same sporangium to analyse the phylogenetic position of Chytriodinium based on SSU and concatenated SSU/LSU rRNA gene sequences, and also characterize two genes related to the plastidial heme pathway, hemL and hemY. The results suggest the presence of a cryptic plastid in Chytriodinium and a photosynthetic ancestral state of the parasitic Chytriodinium/Dissodinium clade. Finally, by mapping Tara Oceans V9 SSU amplicon data to the recovered SSU rRNA gene sequences from the sporocytes, we show that globally, Chytriodinium parasites are most abundant within the pico/nano- and mesoplankton of the surface ocean and almost absent within microplankton, a distribution indicating that they generally exist either as free-living spores or host-associated sporangia.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Chytriodinium_contigs</h4><div class="o-metadata__file-description">Contigs of Chytriodinium cf. affine in FASTA format. Contigs were generated using Ray assembler; contaminating sequences were removed as described in the publication.</div><div class="o-metadata__file-name"></div></div>
Item Metadata
| Title |
Data from: Phylogeny, evidence for a cryptic plastid, and distribution of Chytriodinium parasites (Dinophyceae) infecting copepods
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| Creator | |
| Date Issued |
2021-05-19
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| Description |
<b>Abstract</b><br/>Spores of the dinoflagellate Chytriodinium are known to infest copepod eggs causing their lethality. Despite the potential to control the population of such an ecologically important host, knowledge about Chytriodinium parasites is limited: we know little about phylogeny, parasitism, abundance, or geographical distribution. We carried out genome sequence surveys on four manually isolated sporocytes from the same sporangium to analyse the phylogenetic position of Chytriodinium based on SSU and concatenated SSU/LSU rRNA gene sequences, and also characterize two genes related to the plastidial heme pathway, hemL and hemY. The results suggest the presence of a cryptic plastid in Chytriodinium and a photosynthetic ancestral state of the parasitic Chytriodinium/Dissodinium clade. Finally, by mapping Tara Oceans V9 SSU amplicon data to the recovered SSU rRNA gene sequences from the sporocytes, we show that globally, Chytriodinium parasites are most abundant within the pico/nano- and mesoplankton of the surface ocean and almost absent within microplankton, a distribution indicating that they generally exist either as free-living spores or host-associated sporangia.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Chytriodinium_contigs</h4><div class="o-metadata__file-description">Contigs of Chytriodinium cf. affine in FASTA format. Contigs were generated using Ray assembler; contaminating sequences were removed as described in the publication.</div><div class="o-metadata__file-name"></div></div>
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| Notes |
Dryad version number: 1</p> Version status: submitted</p> Dryad curation status: Published</p> Sharing link: https://datadryad.org/stash/share/gsmVPUKPK5SRr-agnigei37P9J4OPY79q9PJ-pEt_S0</p> Storage size: 131972549</p> Visibility: public</p> |
| Date Available |
2020-06-24
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| Provider |
University of British Columbia Library
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| License |
This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver
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| DOI |
10.14288/1.0397721
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| Aggregated Source Repository |
Dataverse
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Licence
This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver