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Data from: High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying Feng, Jin-Ling; Yang, Zhi-Jian; Chen, Shi-Pin; El-Kassaby, Yousry; Chen, Hui

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<b>Abstract</b><br/>Background: Camellia species are ancient oilseed plants with a history of cultivation over two thousand years. Prior to oil extraction, natural seed drying is often practiced, a process affecting fatty acid quality and quantity. MicroRNAs (miRNA) of lipid metabolism associated with camellia seed natural drying are unexplored. To obtain insight into the function of miRNAs in lipid metabolism during natural drying, Illumina sequencing of C. oleifera and C. meiocarpa small-RNA was conducted. Results: A total of 274 candidate miRNAs were identified and 3733 target unigenes were annotated by performing a BLASTX. Through integrated GO and KEGG function annotation, 23 miRNA regulating 131 target genes were identified as lipid metabolism, regulating fatty acid biosynthesis, accumulation and catabolism. We observed one, two, and four miRNAs of lipid metabolism which were specially expressed in C. Meiocarpa, C. oleifera, and the two species collectively, respectively. At 30% moisture contents, C. meiocarpa and C. oleifer produced nine and eight significant differentially expressed miRNAs, respectively, with high fatty acid synthesis and accumulation activities. Across the two species, 12 significant differentially expressed miRNAs were identified at the 50% moisture content. Conclusions: Sequencing of small-RNA revealed the presence of 23 miRNAs regulating lipid metabolism in camellia seed during natural drying and permitted comparative miRNA profiles between C. Meiocarpa and C. oleifera. Furthermore, this study successfully identified the best drying environment at which the quantity and quality of lipid in camellia seed are at its maximum.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">sequence of miRNAs of camellia species in seed natural drying process</h4><div class="o-metadata__file-description">Camellia miRNAs related to lipid metabolism</div><div class="o-metadata__file-name"></div></div>

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Title	Data from: High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying
Creator	Feng, Jin-Ling; Yang, Zhi-Jian; Chen, Shi-Pin; El-Kassaby, Yousry; Chen, Hui
Date Issued	2021-05-19
Description	<b>Abstract</b><br/>Background: Camellia species are ancient oilseed plants with a history of cultivation over two thousand years. Prior to oil extraction, natural seed drying is often practiced, a process affecting fatty acid quality and quantity. MicroRNAs (miRNA) of lipid metabolism associated with camellia seed natural drying are unexplored. To obtain insight into the function of miRNAs in lipid metabolism during natural drying, Illumina sequencing of C. oleifera and C. meiocarpa small-RNA was conducted. Results: A total of 274 candidate miRNAs were identified and 3733 target unigenes were annotated by performing a BLASTX. Through integrated GO and KEGG function annotation, 23 miRNA regulating 131 target genes were identified as lipid metabolism, regulating fatty acid biosynthesis, accumulation and catabolism. We observed one, two, and four miRNAs of lipid metabolism which were specially expressed in C. Meiocarpa, C. oleifera, and the two species collectively, respectively. At 30% moisture contents, C. meiocarpa and C. oleifer produced nine and eight significant differentially expressed miRNAs, respectively, with high fatty acid synthesis and accumulation activities. Across the two species, 12 significant differentially expressed miRNAs were identified at the 50% moisture content. Conclusions: Sequencing of small-RNA revealed the presence of 23 miRNAs regulating lipid metabolism in camellia seed during natural drying and permitted comparative miRNA profiles between C. Meiocarpa and C. oleifera. Furthermore, this study successfully identified the best drying environment at which the quantity and quality of lipid in camellia seed are at its maximum.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">sequence of miRNAs of camellia species in seed natural drying process</h4><div class="o-metadata__file-description">Camellia miRNAs related to lipid metabolism</div><div class="o-metadata__file-name"></div></div>
Subject	Other; Camellia; miRNAs; natural drying; lipid metabolism
Type	Dataset
Notes	Dryad version number: 1</p> Version status: submitted</p> Dryad curation status: Published</p> Sharing link: https://datadryad.org/stash/share/x-GvuKg8iWSyGDjO8rAkrN2OYQy4SPRGcGdRDXR_vCM</p> Storage size: 40849</p> Visibility: public</p>
Date Available	2020-06-24
Provider	University of British Columbia Library
License	This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver
DOI	10.14288/1.0397697
URI	https://doi.org/10.5683/SP2/GF7OD6
Publisher DOI	https://doi.org/10.5683/SP2/GF7OD6; https://doi.org/10.5061/dryad.1mj01
Rights URI	https://creativecommons.org/publicdomain/zero/1.0/
Aggregated Source Repository	Dataverse

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This dataset is made available under a Creative Commons CC0 license with the following additional/modified terms and conditions: CC0 Waiver