Open Collections

Gerstein, Aleeza C.

<b>Abstract</b><br/>Ploidy is predicted to influence adaptation directly, yet whether single mutations behave the same in different ploidy backgrounds has not been well studied. It has often been assumed theoretically that aside from dominance, selective parameters do not differ between cells of varying ploidy. Using the budding yeast Saccharomyces cerevisiae, I compared the effect size of 20 adaptive mutations in haploids and homozygous diploids and found, surprisingly, that the same mutations often had a much larger effect in haploids than homozygous diploids. This empirical result demonstrates that it cannot be assumed that mutations will have the same effect in haploids and homozygous diploids.; <b>Usage notes</b><br /><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Tolerance parameter estimates from ML</h4><div class="o-metadata__file-name">combined_A_AA_MLc.csv</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">BMN parameters for graphing</h4><div class="o-metadata__file-description">Parameters for data points (colours, position, pch etc.) from Gerstein et al. (Genetics, 2012) dataset.</div><div class="o-metadata__file-name">1207NBMnys_param.csv</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Tolerance log-likelihood results from ML models</h4><div class="o-metadata__file-description">Results from ML models for the three tolerance parameters (IC50, a50, m).</div><div class="o-metadata__file-name">NysIC50_A_AA_modelsig.csv</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">R code to produce Figure1 (Tolerance data)</h4><div class="o-metadata__file-name">1209 Figure1.R</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">R code to produce Figure2 (Bioscreen data)</h4><div class="o-metadata__file-description">R code for Figure 2. Includes statistical analysis for the bioscreen data.</div><div class="o-metadata__file-name">1207 Figure2_GR.R</br></div></div><div class="o-metadata__file-usage-entry"><h4 class="o-heading__level3-file-title">Bioscreen data</h4><div class="o-metadata__file-description">Growth rate data required to produce Figure 2.</div><div class="o-metadata__file-name">A_AA_rawdata.csv</br></div></div>

Dataset

2020-06-30

CC0 1.0

https://doi.org/10.5683/SP2/C9CM31

http://creativecommons.org/publicdomain/zero/1.0