UBC Research Data

Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans. McDiarmid, Troy; Au, Vinci; Moerman, Don; Rankin, Catharine

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Raw and processed data for "Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans." This includes data demonstrating the effectiveness of peel-1 negative selection on improving CRISPR-Cas9 genome editing, and functional data investigating the roles of two genes using Multi-Worm Tracker software. Further details about this project, including a README.md, paper abstract, and the R code necessary to regenerate all of the figures and formal analyses in the manuscript using the data in this repository can be found at: https://github.com/troymcdiarmid/peel-1 The "peel1_dels_0306_2020" folder includes all of the raw Multi-Worm Tracker (https://sourceforge.net/projects/mwt/) output files. The files are organized to have one folder per tracked plate (time stamped). The organized morphology summary (data.smorph) and reversal feature summaries (data.srev) are also included in case the user does not want to regenerate them using the code at the associated github (included above) The "peel_selection_data" folder includes raw data describing the effectiveness of peel-1 and selecting against extrachromosomal array transgenics during genome editing (see the "peel1_analysis" markdown document at the associated github page for further details).

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