- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Role of intercellular adhesion molecule 2(ICAM-2) in...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Role of intercellular adhesion molecule 2(ICAM-2) in the murine immune system Carpenito, Carmine
Abstract
Intercellular adhesion molecule-2 (ICAM-2; CD102) is one of three ligands for the p2 leukocyte integrin LFA-1 (CD11a/CD18). Although ICAM-2 expression is limited to lymphocytes, monocytes, granulocytes, and endothelium, the biological role of ICAM-2 has remained unknown. In this thesis, the murine ICAM-2 cDNA was cloned in order to assist the functional investigation. Sequence analysis of both the cDNA and the genomic clone revealed that ICAM-2 is a member of the immunoglobulin superfamily. The cDNA and antibody were used to examine the role of ICAM-2 in T cell activation and leukocyte transendothelial migration. In order to examine the role of ICAM-2 in antigen presentation to T cells, murine fibroblastic L cells expressing allogeneic class II MHC (l-Ed) were transfected with the ICAM-2 cDNA and tested for the ability to stimulate splenic T cells. The expression of ICAM-2 significantly increased the stimulation of T cells in an LFA-1-dependent manner. The increased T cell response was also observed when the class II MHC and ICAM-2 were expressed in separate cells combined together. This indicated that ICAM-2 is actually transmitting a costimulatory signal rather than merely enhancing T cell adhesion to the antigen presenting cell. T cells stimulated with ICAM-2-transfected L cells expressing the class II MHC were able to respond to an allogeneic secondary stimulation. In contrast, T cells stimulated with L cells expressing only the class II MHC were not able to respond to allogeneic stimulation in the secondary response. These results indicated that ICAM-2 may provide a necessary costimulatory signal to the T cell that is required for the aversion of an anergic state. Endothelial cells transfected with the ICAM-2 cDNA were examined for the ability to assist leukocyte migration. A system was set up in which transendothelial migration could be easily quantitated. It was found that a lymphocytic cell line and bone marrow neutrophils were able to utilize ICAM-2 for the migratory process without destroying the endothelial monolayer. These results demonstrate that ICAM-2 is able to play a role in two physiological processes that are of central importance to the normal function of the immune system.
Item Metadata
Title |
Role of intercellular adhesion molecule 2(ICAM-2) in the murine immune system
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
1997
|
Description |
Intercellular adhesion molecule-2 (ICAM-2; CD102) is one of three ligands for the p2
leukocyte integrin LFA-1 (CD11a/CD18). Although ICAM-2 expression is limited to
lymphocytes, monocytes, granulocytes, and endothelium, the biological role of ICAM-2 has
remained unknown. In this thesis, the murine ICAM-2 cDNA was cloned in order to assist the
functional investigation. Sequence analysis of both the cDNA and the genomic clone revealed
that ICAM-2 is a member of the immunoglobulin superfamily. The cDNA and antibody were
used to examine the role of ICAM-2 in T cell activation and leukocyte transendothelial
migration.
In order to examine the role of ICAM-2 in antigen presentation to T cells, murine
fibroblastic L cells expressing allogeneic class II MHC (l-Ed) were transfected with the ICAM-2
cDNA and tested for the ability to stimulate splenic T cells. The expression of ICAM-2
significantly increased the stimulation of T cells in an LFA-1-dependent manner. The
increased T cell response was also observed when the class II MHC and ICAM-2 were
expressed in separate cells combined together. This indicated that ICAM-2 is actually
transmitting a costimulatory signal rather than merely enhancing T cell adhesion to the antigen
presenting cell. T cells stimulated with ICAM-2-transfected L cells expressing the class II MHC
were able to respond to an allogeneic secondary stimulation. In contrast, T cells stimulated
with L cells expressing only the class II MHC were not able to respond to allogeneic stimulation
in the secondary response. These results indicated that ICAM-2 may provide a necessary
costimulatory signal to the T cell that is required for the aversion of an anergic state.
Endothelial cells transfected with the ICAM-2 cDNA were examined for the ability to
assist leukocyte migration. A system was set up in which transendothelial migration could be easily quantitated. It was found that a lymphocytic cell line and bone marrow neutrophils were
able to utilize ICAM-2 for the migratory process without destroying the endothelial monolayer.
These results demonstrate that ICAM-2 is able to play a role in two physiological
processes that are of central importance to the normal function of the immune system.
|
Extent |
9045673 bytes
|
Genre | |
Type | |
File Format |
application/pdf
|
Language |
eng
|
Date Available |
2009-04-03
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0088063
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
1997-11
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.