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Functional characterization of mfs (2) 31 : a recessive supressor of position effect variegation

University of British Columbia

The Drosophila melanogaster gene, mfs(2)31, is a recessive suppressor of position effect variegation (PEV) and has two open reading frames (ORFs) putatively derived from a single heterogeneous nuclear RNA (hnRNA). The coding region of Intronic Centrosomal Protein (ICP) is located in the first intron of the ubiquitin 80 amino acid fusion protein (DUb80) transcript, and is known to be liberated as a 1.2 Kb polyadenylated mRNA. Antibodies raised against the C-terminal 85 amino acids of ICP were used as a reagent for western blotting and indirect immunofluorescent staining of Drosophila embryos and HeLa cells. ICP was found to co-localize with p- tubulin in a Triton X-100® resistant compartment of both the Drosophila embryonic centrosome, and the HeLa cell centrosome. ICP was also localized to small, discrete, Triton X-100® resistant 'flecks' within HeLa cell nuclei in a pattern suggestive of the nuclear scaffold. These data suggest that ICP is involved in modulating nuclear and cytoplasmic microtubule dynamics throughout the cell cycle.

Thesis/Dissertation

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2009-01-18

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http://hdl.handle.net/2429/3763

Zoology

University of British Columbia

UBCV

DSpace