- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Theses and Dissertations /
- Enhanced yield of medicinal products from Tripterygium...
Open Collections
UBC Theses and Dissertations
UBC Theses and Dissertations
Enhanced yield of medicinal products from Tripterygium cell cultures Samija, Mijo Daniel
Abstract
The growth of cell suspensions of the Asian medicinal plant Tripterygium wilfordii was manipulated in order to produce large amounts of pharmacologically active diterpene and triterpene natural products. A method was developed where elicitation with a strain of the fungus Botrytis stimulated the production of oleanane and friedelane triterpene acids. In rapidly growing twelve liter bioreactor cultures, triterpene yields were increased five to ten fold with this process, routinely providing more than 25 mg/L each of 22α-hydroxy-3-oxoolean-12-en-29-oic acid (B) and 3β,22α-dihydroxyolean-12-en-29-oic acid (D) and more than 5 mg/L each of 22β-hydroxy-3-oxoolean-12-en-29-oic acid (A) and 3β,22β-dihydroxyolean-12-en-29-oic acid (C). Yield improvement for the triptolide family of diterpenes (triptolide and tripdiolide) was approached through the synthesis of potential intermediates of the natural biosynthetic pathways, the first step in a technique where synthetic elaborations would be completed by cell cultures. These synthetic intermediates were also sought to establish the details of triptolide biosynthesis. Advances were made towards the synthesis of one potential intermediate, 18(4→ 3)-isodehydroabietenolide, starting from (L)-dehydroabietic acid....(more text)
Item Metadata
Title |
Enhanced yield of medicinal products from Tripterygium cell cultures
|
Creator | |
Publisher |
University of British Columbia
|
Date Issued |
1992
|
Description |
The growth of cell suspensions of the Asian medicinal plant Tripterygium wilfordii was manipulated in order to produce large amounts of pharmacologically active diterpene and triterpene natural products. A method was developed where elicitation with a strain of the fungus Botrytis stimulated the production of oleanane and friedelane triterpene acids. In rapidly growing twelve liter bioreactor cultures, triterpene yields were increased five to ten fold with this process, routinely providing more than 25 mg/L each of 22α-hydroxy-3-oxoolean-12-en-29-oic acid (B) and 3β,22α-dihydroxyolean-12-en-29-oic acid (D) and more than 5 mg/L each of 22β-hydroxy-3-oxoolean-12-en-29-oic acid (A) and 3β,22β-dihydroxyolean-12-en-29-oic acid (C).
Yield improvement for the triptolide family of diterpenes (triptolide and tripdiolide) was approached through the synthesis of potential intermediates of the natural biosynthetic pathways, the first step in a technique where synthetic elaborations would be completed by cell cultures. These synthetic intermediates were also sought to establish the details of triptolide biosynthesis. Advances were made towards the synthesis of one potential intermediate,
18(4→ 3)-isodehydroabietenolide, starting from (L)-dehydroabietic acid....(more text)
|
Extent |
6864089 bytes
|
Genre | |
Type | |
File Format |
application/pdf
|
Language |
eng
|
Date Available |
2008-09-18
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
|
DOI |
10.14288/1.0061785
|
URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
|
Graduation Date |
1992-05
|
Campus | |
Scholarly Level |
Graduate
|
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.