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UBC Theses and Dissertations
Silica based immunoassays for a covalently attached antigen Melzak, Kathryn
Abstract
An immunoassay was developed using a monoclonal antibody and anantigen covalently immobilized on silica, showing that silica is a potentially useful assay substrate. Silica beads prepared from tetraethyl orthosilicate (0.78 pmdiameter) and fused quartz slides were thoroughly cleaned and modified with 3-aminopropyltriethoxysilane. Amine groups on the modified slides and beads were used for carbodiimide mediated coupling to carboxyl groups on a peptide antigen. High surface concentrations of antigen were achieved. Antibody binding to the antigen-modified beads and slides was measured using an iodinated monoclonal antibody. The equilibrium constant for the antibody binding on the beads was ten times higher than on the flat silica, but the maximum amount bound was lower. Immunoassays performed using a second enzyme-labelled antibody to detect the monoclonal antibody bound to the silica permitted detection of the antigen at 10-8 M using the modified beads and at10-7 M using the slides.
Item Metadata
Title |
Silica based immunoassays for a covalently attached antigen
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Creator | |
Publisher |
University of British Columbia
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Date Issued |
1993
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Description |
An immunoassay was developed using a monoclonal antibody and anantigen covalently immobilized on silica, showing that silica is a potentially useful assay substrate.
Silica beads prepared from tetraethyl orthosilicate (0.78 pmdiameter) and fused quartz slides were thoroughly cleaned and modified with 3-aminopropyltriethoxysilane. Amine groups on the modified slides and beads were used for carbodiimide mediated coupling to carboxyl groups on a peptide antigen. High surface concentrations of antigen were achieved. Antibody binding to the antigen-modified beads and slides was measured using an iodinated monoclonal antibody. The equilibrium constant for the antibody binding on the beads was ten times higher than on the flat silica, but the maximum amount bound was lower. Immunoassays performed using a second enzyme-labelled antibody to detect the monoclonal antibody bound to the silica permitted detection of the antigen at 10-8 M using the modified beads and at10-7 M using the slides.
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Extent |
6850939 bytes
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Genre | |
Type | |
File Format |
application/pdf
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Language |
eng
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Date Available |
2008-09-11
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Provider |
Vancouver : University of British Columbia Library
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Rights |
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
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DOI |
10.14288/1.0061779
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
1993-11
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Campus | |
Scholarly Level |
Graduate
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Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.