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A new process for the separation and purification of egg yolk antibodies Charter, Edward

Abstract

A three stage process was developed for the separation of an antibody (IgY) from industrially separated chicken egg yolk. This included aqueous extraction of the water soluble fraction (WSF) from the yolk by dilution with distilled water and pH adjustment, separation of IgY from the WSF using a cation exchange column in an automated chromatography system, and finally purification of IgY using precipitation with sodium sulphate. The overall recovery of the process was approximately 50%, and the purity 95% or greater depending on the number of precipitation steps used. An automated liquid chromatography system was developed to allow efficient study of various chromatographic media for the separation of the antibody. Analysis of breakthrough curves indicates that superficial velocity is the governing parameter in the binding of IgY to the cation exchanger. Results of a pilot scale experiment involving the application of 46.5 litres of WSF containing approximately 0.8 mg/ml IgY to a 1500 nil column are presented.

Item Metadata

Title
A new process for the separation and purification of egg yolk antibodies
Creator
Charter, Edward
Publisher
University of British Columbia
Date Issued
1993
Description
A three stage process was developed for the separation of an antibody (IgY) from industrially separated chicken egg yolk. This included aqueous extraction of the water soluble fraction (WSF) from the yolk by dilution with distilled water and pH adjustment, separation of IgY from the WSF using a cation exchange column in an automated chromatography system, and finally purification of IgY using precipitation with sodium sulphate. The overall recovery of the process was approximately 50%, and the purity 95% or greater depending on the number of precipitation steps used. An automated liquid chromatography system was developed to allow efficient study of various chromatographic media for the separation of the antibody. Analysis of breakthrough curves indicates that superficial velocity is the governing parameter in the binding of IgY to the cation exchanger. Results of a pilot scale experiment involving the application of 46.5 litres of WSF containing approximately 0.8 mg/ml IgY to a 1500 nil column are presented.
Extent
6101435 bytes
Genre
Thesis/Dissertation
Type
Text
File Format
application/pdf
Language
eng
Date Available
2008-09-12
Provider
Vancouver : University of British Columbia Library
Rights
For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
DOI
10.14288/1.0058569
URI
http://hdl.handle.net/2429/1917
Degree
Doctor of Philosophy - PhD
Program
Chemical and Biological Engineering
Affiliation
Applied Science, Faculty of; Chemical and Biological Engineering, Department of
Degree Grantor
University of British Columbia
Graduation Date
1993-05
Campus
UBCV
Scholarly Level
Graduate
Aggregated Source Repository
DSpace

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For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.