[{"key":"dc.contributor","value":"University of British Columbia. Centre for Heart Lung Innovation","language":"en"},{"key":"dc.contributor.author","value":"Shahzad, Affan Mahmood","language":""},{"key":"dc.contributor.author","value":"Lu, Wenying","language":""},{"key":"dc.contributor.author","value":"Dey, Surajit","language":""},{"key":"dc.contributor.author","value":"Bhattarai, Prem","language":""},{"key":"dc.contributor.author","value":"Gaikwad, Archana Vijay","language":""},{"key":"dc.contributor.author","value":"Jaffar, Jade","language":""},{"key":"dc.contributor.author","value":"Westall, Glen","language":""},{"key":"dc.contributor.author","value":"Sutherland, Darren","language":""},{"key":"dc.contributor.author","value":"Singhera, Gurpreet K.","language":""},{"key":"dc.contributor.author","value":"Hackett, Tillie-Louise","language":""},{"key":"dc.contributor.author","value":"Eapen, Mathew Suji","language":""},{"key":"dc.contributor.author","value":"Sohal, Sukhwinder Singh","language":""},{"key":"dc.date.accessioned","value":"2024-04-26T16:16:20Z","language":""},{"key":"dc.date.available","value":"2024-04-26T16:16:22Z","language":""},{"key":"dc.date.issued","value":"2024-04-06","language":""},{"key":"dc.identifier","value":"10.3390\/jcm13072126","language":"en"},{"key":"dc.identifier.citation","value":"Journal of Clinical Medicine 13 (7): 2126 (2024)","language":"en"},{"key":"dc.identifier.uri","value":"http:\/\/hdl.handle.net\/2429\/88014","language":""},{"key":"dc.description.abstract","value":"Background: Idiopathic pulmonary fibrosis (IPF) is an irreversible lung fibrotic disorder of unknown cause. It has been reported that bacterial and viral co-infections exacerbate disease pathogenesis. These pathogens use adhesion molecules such as platelet activating factor receptor (PAFR) and intercellular adhesion molecule-1 (ICAM\u20131) to gain cellular entry, causing infections. Methods: Immunohistochemical staining was carried out for lung resections from IPF patients (n = 11) and normal controls (n = 12). The quantification of PAFR and ICAM\u20131 expression is presented as a percentage in the small airway epithelium. Also, type 2 pneumocytes and alveolar macrophages were counted as cells per mm2 of the parenchymal area and presented as a percentage. All image analysis was done using Image Pro Plus 7.0 software. Results: PAFR expression significantly increased in the small airway epithelium (p < 0.0001), type 2 pneumocytes (p < 0.0001) and alveolar macrophages (p < 0.0001) compared to normal controls. Similar trend was observed for ICAM\u20131 expression in the small airway epithelium (p < 0.0001), type 2 pneumocytes (p < 0.0001) and alveolar macrophages (p < 0.0001) compared to normal controls. Furthermore, the proportion of positively expressed type 2 pneumocytes and alveolar macrophages was higher in IPF than in normal control. Conclusions: This is the first study to show PAFR and ICAM\u20131 expression in small airway epithelium, type 2 pneumocytes and alveolar macrophages in IPF. These findings could help intervene microbial impact and facilitate management of disease pathogenesis.","language":"en"},{"key":"dc.language.iso","value":"eng","language":"en"},{"key":"dc.publisher","value":"Multidisciplinary Digital Publishing Institute","language":"en"},{"key":"dc.rights","value":"CC BY 4.0","language":""},{"key":"dc.rights.uri","value":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","language":""},{"key":"dc.subject","value":"alveolar macrophages","language":"en"},{"key":"dc.subject","value":"ICAM\u20131","language":"en"},{"key":"dc.subject","value":"idiopathic pulmonary fibrosis","language":"en"},{"key":"dc.subject","value":"infections","language":"en"},{"key":"dc.subject","value":"PAFR","language":"en"},{"key":"dc.subject","value":"type 2 pneumocytes","language":"en"},{"key":"dc.title","value":"Platelet Activating Factor Receptor and Intercellular Adhesion Molecule\u20131 Expression Increases in the Small Airway Epithelium and Parenchyma of Patients with Idiopathic Pulmonary Fibrosis : Implications for Microbial Pathogenesis","language":"en"},{"key":"dc.type","value":"Text","language":"en"},{"key":"dc.type.text","value":"Article","language":"en"},{"key":"dc.description.affiliation","value":"Medicine, Faculty of","language":"en"},{"key":"dc.description.affiliation","value":"Non UBC","language":"en"},{"key":"dc.description.affiliation","value":"Anesthesiology, Pharmacology and Therapeutics, Department of","language":"en"},{"key":"dc.description.reviewstatus","value":"Reviewed","language":"en"},{"key":"dc.description.scholarlevel","value":"Faculty","language":"en"},{"key":"dc.description.scholarlevel","value":"Researcher","language":"en"},{"key":"dc.date.updated","value":"2024-04-12T13:14:37Z","language":""}]