[{"key":"dc.contributor.author","value":"Zamani, Khosro","language":null},{"key":"dc.contributor.author","value":"Allah-Bakhshi, Noushin","language":null},{"key":"dc.contributor.author","value":"Akhavan, Faezeh","language":null},{"key":"dc.contributor.author","value":"Yousefi, Mahdieh","language":null},{"key":"dc.contributor.author","value":"Golmoradi, Rezvan","language":null},{"key":"dc.contributor.author","value":"Ramezani, Moazzameh","language":null},{"key":"dc.contributor.author","value":"Bach, Horacio","language":null},{"key":"dc.contributor.author","value":"Razavi, Shabnam","language":null},{"key":"dc.contributor.author","value":"Irajian, Gholam-Reza","language":null},{"key":"dc.contributor.author","value":"Gerami, Mahyar","language":null},{"key":"dc.contributor.author","value":"Pakdin-Parizi, Ali","language":null},{"key":"dc.contributor.author","value":"Tafrihi, Majid","language":null},{"key":"dc.contributor.author","value":"Ramezani, Fatemeh","language":null},{"key":"dc.date.accessioned","value":"2022-01-19T19:13:04Z","language":null},{"key":"dc.date.available","value":"2022-01-19T19:13:04Z","language":null},{"key":"dc.date.issued","value":"2021-12-07","language":null},{"key":"dc.identifier","value":"10.1186\/s12896-021-00727-1","language":"en"},{"key":"dc.identifier.citation","value":"BMC Biotechnology. 2021 Dec 07;21(1):68","language":"en"},{"key":"dc.identifier.uri","value":"http:\/\/hdl.handle.net\/2429\/80686","language":null},{"key":"dc.description.abstract","value":"Background\r\n                Antibiotics have been widely used for the treatment of bacterial infections for decades. However, the rapid emergence of antibiotic-resistant bacteria has created many problems with a heavy burden for the medical community. Therefore, the use of nanoparticles as an alternative for antibacterial activity has been explored. In this context, metal nanoparticles have demonstrated broad-spectrum antimicrobial activity. This study investigated the antimicrobial activity of naked cerium oxide nanoparticles dispersed in aqueous solution (CNPs) and surface-stabilized using\u00a0Pseudomonas aeruginosa\u00a0as a bacterial model.\r\n              \r\n              \r\n                Methods\r\n                Gelatin-polycaprolactone nanofibers containing CNPs (Scaffold@CNPs) were synthesized, and their effect on P. aeruginosa was investigated. The minimum inhibitory and bactericidal concentrations of the nanoparticls were determined in an ATCC reference strain and a clinical isolate strain. To determine whether the exposure to the nanocomposites might change the expression of antibiotic resistance, the expression of the genes shv, kpc, and imp was also investigated. Moreover, the cytotoxicity of the CNPs was assessed on fibroblast using flow cytometry.\r\n              \r\n              \r\n                Results\r\n                Minimum bactericidal concentrations for the ATCC and the clinical isolate of 50\u00a0\u00b5g\/mL and 200\u00a0\u00b5g\/mL were measured, respectively, when the CNPs were used. In the case of the Scaffold@CNPs, the bactericidal effect was 50\u00a0\u00b5g\/mL and 100\u00a0\u00b5g\/mL for the ATCC and clinical isolate, respectively. Interestingly, the exposure to the Scaffold@CNPs significantly decreased the expression of the genes shv, kpc, and imp.\r\n              \r\n              \r\n                Conclusions\r\n                A concentration of CNPs and scaffold@CNPs higher than 50\u00a0\u03bcg\/mL can be used to inhibit the growth of P. aeruginosa. The fact that the scaffold@CNPs significantly reduced the expression of resistance genes, it has the potential to be used for medical applications such as wound dressings.","language":"en"},{"key":"dc.language.iso","value":"eng","language":"en"},{"key":"dc.publisher","value":"BioMed Central","language":"en"},{"key":"dc.rights","value":"Attribution 4.0 International (CC BY 4.0)","language":null},{"key":"dc.rights.uri","value":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/","language":null},{"key":"dc.subject","value":"Cerium oxide nanoparticles","language":"en"},{"key":"dc.subject","value":"Nanofiber","language":"en"},{"key":"dc.subject","value":"Antibiotic resistance","language":"en"},{"key":"dc.subject","value":"Pseudomonas aeruginosa","language":"en"},{"key":"dc.subject","value":"Gene expression","language":"en"},{"key":"dc.subject","value":"Cytotoxicity","language":"en"},{"key":"dc.subject","value":"Clinical isolate","language":"en"},{"key":"dc.title","value":"Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa","language":"en"},{"key":"dc.type","value":"Text","language":"en"},{"key":"dc.type.text","value":"Article","language":"en"},{"key":"dc.description.affiliation","value":"Medicine, Faculty of","language":"en"},{"key":"dc.description.affiliation","value":"Non UBC","language":"en"},{"key":"dc.description.affiliation","value":"Medicine, Department of","language":"en"},{"key":"dc.description.reviewstatus","value":"Reviewed","language":"en"},{"key":"dc.rights.copyright","value":"The Author(s)","language":"en"},{"key":"dc.description.scholarlevel","value":"Faculty","language":"en"},{"key":"dc.description.scholarlevel","value":"Researcher","language":"en"},{"key":"dc.date.updated","value":"2021-12-07T15:02:06Z","language":null}]