[{"key":"dc.contributor","value":"Michael Smith Laboratories","language":"en"},{"key":"dc.contributor.author","value":"Akbari, Vahid","language":""},{"key":"dc.contributor.author","value":"Garant, Jean-Michel","language":""},{"key":"dc.contributor.author","value":"O\u2019Neill, Kieran","language":""},{"key":"dc.contributor.author","value":"Pandoh, Pawan","language":""},{"key":"dc.contributor.author","value":"Moore, Richard","language":""},{"key":"dc.contributor.author","value":"Marra, Marco, 1966-","language":""},{"key":"dc.contributor.author","value":"Hirst, Martin","language":""},{"key":"dc.contributor.author","value":"Jones, Steven J. M.","language":""},{"key":"dc.date.accessioned","value":"2021-02-24T18:52:32Z","language":""},{"key":"dc.date.available","value":"2021-02-24T18:52:33Z","language":""},{"key":"dc.date.issued","value":"2021-02-22","language":""},{"key":"dc.identifier","value":"10.1186\/s13059-021-02283-5","language":"en"},{"key":"dc.identifier.citation","value":"Genome Biology. 2021 Feb 22;22(1):68","language":"en"},{"key":"dc.identifier.uri","value":"http:\/\/hdl.handle.net\/2429\/77374","language":""},{"key":"dc.description.abstract","value":"The ability of nanopore sequencing to simultaneously detect modified nucleotides while producing long reads makes it ideal for detecting and phasing allele-specific methylation. However, there is currently no complete software for detecting SNPs, phasing haplotypes, and mapping methylation to these from nanopore sequence data. Here, we present NanoMethPhase, a software tool to phase 5-methylcytosine from nanopore sequencing. We also present SNVoter, which can post-process nanopore SNV calls to improve accuracy in low coverage regions. Together, these tools can accurately detect allele-specific methylation genome-wide using nanopore sequence data with low coverage of about ten-fold redundancy.","language":"en"},{"key":"dc.language.iso","value":"eng","language":"en"},{"key":"dc.publisher","value":"BioMed Central","language":"en"},{"key":"dc.rights","value":"Attribution 4.0 International (CC BY 4.0)","language":""},{"key":"dc.rights.uri","value":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/","language":""},{"key":"dc.subject","value":"Nanopore sequencing","language":"en"},{"key":"dc.subject","value":"Allele-specific methylation","language":"en"},{"key":"dc.subject","value":"Phasing","language":"en"},{"key":"dc.subject","value":"NanoMethPhase","language":"en"},{"key":"dc.title","value":"Megabase-scale methylation phasing using nanopore long reads and NanoMethPhase","language":"en"},{"key":"dc.type","value":"Text","language":"en"},{"key":"dc.type.text","value":"Article","language":"en"},{"key":"dc.description.affiliation","value":"Medicine, Faculty of","language":"en"},{"key":"dc.description.affiliation","value":"Science, Faculty of","language":"en"},{"key":"dc.description.affiliation","value":"Other UBC","language":"en"},{"key":"dc.description.affiliation","value":"Non UBC","language":"en"},{"key":"dc.description.affiliation","value":"Medical Genetics, Department of","language":"en"},{"key":"dc.description.affiliation","value":"Microbiology and Immunology, Department of","language":"en"},{"key":"dc.description.reviewstatus","value":"Reviewed","language":"en"},{"key":"dc.rights.copyright","value":"The Author(s)","language":"en"},{"key":"dc.description.scholarlevel","value":"Faculty","language":"en"},{"key":"dc.date.updated","value":"2021-02-22T12:09:59Z","language":""}]