[{"key":"dc.contributor.author","value":"Wei, Ziwang","language":null},{"key":"dc.date.accessioned","value":"2024-04-10T17:18:49Z","language":null},{"key":"dc.date.available","value":"2026-04-30T07:00:00Z","language":null},{"key":"dc.date.issued","value":"2024","language":"en"},{"key":"dc.identifier.uri","value":"http:\/\/hdl.handle.net\/2429\/87728","language":null},{"key":"dc.description.abstract","value":"Natural products are a chemically diverse group of molecules that are isolated from bacteria, fungi, or plants. Many natural products can be employed as active pharmaceutical agents, among which there is a subset bearing N-heterocycles or diazo groups in their structures. In this thesis, I have been studying the biosynthetic assembly of two types of heterocycle-containing molecules and one type of diazo compound. In the first chapter of my thesis, I review the research background of these three molecules: piperazic acid, azaserine, and azomycin. In the second chapter of my thesis, I describe my work on piperazic acid, which for the first time reveals how this cyclic hydrazine \u03b1-amino acid is activated for incorporation into diverse nonribosomal peptidyl natural products by employing a strain named Streptomyces incarnatus NRRL 8089 and its putative incarnatapeptin gene cluster. In the third chapter of my thesis, I discuss the biosynthetic studies of the diazo \u03b1-amino acid azaserine in Glycomyces harbinensis DSM46494, implicating a novel pathway for diazo group formation in nature. In the fourth chapter of my thesis, I write about my X-ray crystallographic studies on two azomycin biosynthetic enzymes from the Pseudomonas genus \u2212 RohQ and RohS \u2212 featuring a cyclodehydratase catalyzing spontaneous cyclodehydration reaction and an enzyme belonging to the emerging family of heme oxygenase-like \u1e0fiiron oxidase\/oxygenase (HDO) that catalyzes a six-electron oxidation, respectively. In the concluding chapter of my thesis, I summarize my work on these three research topics and discuss their future directions.","language":"en"},{"key":"dc.language.iso","value":"eng","language":"en"},{"key":"dc.publisher","value":"University of British Columbia","language":"en"},{"key":"dc.rights","value":"Attribution-NonCommercial-NoDerivatives 4.0 International","language":"*"},{"key":"dc.rights.uri","value":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/","language":"*"},{"key":"dc.title","value":"Biosynthetic enzymes for assembly of nonproteinogenic \u03b1-amino acids piperazic acid and azaserine and heterocyclic natural product azomycin","language":"en"},{"key":"dc.type","value":"Text","language":"en"},{"key":"dc.degree.name","value":"Doctor of Philosophy - PhD","language":"en"},{"key":"dc.degree.discipline","value":"Chemistry","language":"en"},{"key":"dc.degree.grantor","value":"University of British Columbia","language":"en"},{"key":"dc.contributor.supervisor","value":"Ryan, Katherine S.","language":null},{"key":"dc.date.graduation","value":"2024-05","language":"en"},{"key":"dc.type.text","value":"Thesis\/Dissertation","language":"en"},{"key":"dc.description.affiliation","value":"Science, Faculty of","language":"en"},{"key":"dc.description.affiliation","value":"Chemistry, Department of","language":"en"},{"key":"dc.degree.campus","value":"UBCV","language":"en"},{"key":"dc.description.scholarlevel","value":"Graduate","language":"en"}]