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van Wersch, Rowan

University of British Columbia

A diverse array of defense mechanisms and signaling pathways are activated following the detection of pathogens by the plant immune system. Immunity is kept under tight regulation in order to respond appropriately to threats while avoiding costly autoimmune activity in the absence of pathogens. ANP2 (Arabidopsis NPK1-related Protein kinase 2) and ANP3 (Arabidopsis NPK1-related Protein kinase 3) operate as part of a mitogen-activated protein kinase (MAPK) cascade that modulates downstream responses to pathogen attack. Loss of ANP2 and ANP3 leads to autoimmunity and dwarfism. In this study we investigate a large number of suppressor of anp2/anp3 (san) mutants in the anp2/anp3 double mutant background. We show that these san mutants suppress the morphological deficiencies exhibited by the anp2/anp3 mutant. In addition, the san mutants show broad suppression of the autoimmunity present in the anp2/anp3 double mutant, suggesting that these mutations are located within genes that encode immune-relevant proteins, likely downstream components of the same signaling pathway that ANP2 and ANP3 operate in. We mapped these mutations and identified three genomic regions containing candidate genes of interest.

Text

2024-01-08

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/87154

Botany

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/