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Song, Jun Yong

University of British Columbia

Preclinical studies provide valuable insights into disease mechanisms, potential treatments, and therapeutic strategies before clinical trials in the studies of retinal diseases. Mouse, one of the most widely used small animals for preclinical studies, possess great advantages as experimental animals based on their easy accessibility and availability which make them an ideal animal with a broad range of pathological models. Optical coherence tomography (OCT) allows non-invasive in vivo imaging that generates high-resolution 3D images and has been successfully applied in ophthalmology. Also, the recent progress in the development of functional extensions of OCT allowed the generation of multiple contrasts providing information-rich images. This thesis focuses on the development and verification of a polarization-diversity OCT (PD-OCT) system tailored for the mouse retina to acquire high-resolution multi-contrast images. The implementation of a sensorless adaptive optics technique and a multiple spectrometer calibration method ensures the capture of retinal images with an advanced resolution while precisely detecting melanin contrast. Preclinical application of PD-OCT demonstrated its efficacy in visualizing fine retinal features in multiple contrasts with the help of the 3D image registration technique.

Text

2024-01-08

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/87161

Biomedical Engineering

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/