Open Collections

Abbasidezfouli, Samin

University of British Columbia

Background: Based on the WHO report in 2018, 11 million burn injuries occur worldwide annually, 180,000 of which are lethal. Many studies over the past few years have demonstrated that infection and delayed wound healing are responsible for 42%–65% mortality in burn victims. Hence, the development and application of an antibacterial nutritional liquid scaffold in large burn injuries could reduce the infection rate, accelerate wound healing, and ultimately, save lives. MeshFill is a nutritional liquid scaffold found to improve wound healing and esthetic outcomes upon application in a split-thickness meshed skin graft (STMSG) in a porcine model. To expand the efficacy of MeshFill and address the prevention of infections due to wound contamination, our group added antimicrobial properties by replacing polyvinyl alcohol (PVA), a component in the MeshFill complex, with Povidone-iodine (PVP-I). Unlike silver and antibiotics, low concentrations of iodine are not toxic to human cells, and there is no potential for bacterial resistance. Experimental approach: We assessed the antibacterial effect of various ratios of PVP-I/PVP scaffoderm on both gram-positive and gram-negative bacteria during the 4- and 24-hour time points. The cytotoxicity of the three best ratios of PVP-I/PVP scaffoderm was assessed in vitro, while the wound healing effects in terms of antibacterial activity were then evaluated in vivo using relevant rat models. Result: In terms of antibacterial activity, 150% and 200% PVP-I/PVP scaffoderm was shown to kill gram-positive and gram-negative species most effectively in comparison with other groups. Moreover, 100% and 150% PVP-I/PVP scaffoderm showed the best healing results in vivo and did not delay the wound healing process. Conclusion: Scaffoderm containing 150% PVP-I/PVP is most efficacious in terms of antibacterial activity in full-thickness wounds and the promotion of wound healing. The incorporation of PVP-I into MeshFill may be an effective method to prevent impaired wound healing caused by bacterial infection.

Text

2023-06-28

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/85166

Experimental Medicine

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/