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UBC Theses and Dissertations
Discovery and characterization of novel manganese-selective cation diffusion facilitators as putative anthelminthic drug targets in Caenorhabditis elegans Au, Vinci
Abstract
Parasitic nematodes infect over 1.5 billion people worldwide and are a burden on the agricultural and veterinary industries. Widespread use of the few available classes of anthelmintics have led to the development of anthelmintic resistance amongst these parasitic helminths. It is imperative that we find new therapeutics to address the looming threat of anthelminthic resistance. One method for developing new chemotherapies is through rational drug design. Using C. elegans as a surrogate for parasitic nematodes, we have taken the first steps to identify and characterize a small group of putatively druggable “nematode-specific” proteins by generating null mutants and endogenously-tagged reporters using CRISPR-Cas9 gene-editing technology. To our knowledge, this work is the first to define a family of novel manganese-selective cation diffusion facilitators in C. elegans. Although these are not essential proteins and are unlikely to be good drug targets, their newly discovered role in manganese tolerance advances our knowledge of the mechanisms that underlie manganese biology and toxicity.
Item Metadata
Title |
Discovery and characterization of novel manganese-selective cation diffusion facilitators as putative anthelminthic drug targets in Caenorhabditis elegans
|
Creator | |
Supervisor | |
Publisher |
University of British Columbia
|
Date Issued |
2021
|
Description |
Parasitic nematodes infect over 1.5 billion people worldwide and are a burden on the agricultural
and veterinary industries. Widespread use of the few available classes of anthelmintics have led
to the development of anthelmintic resistance amongst these parasitic helminths. It is imperative
that we find new therapeutics to address the looming threat of anthelminthic resistance. One
method for developing new chemotherapies is through rational drug design. Using C. elegans as
a surrogate for parasitic nematodes, we have taken the first steps to identify and characterize a
small group of putatively druggable “nematode-specific” proteins by generating null mutants and
endogenously-tagged reporters using CRISPR-Cas9 gene-editing technology. To our knowledge,
this work is the first to define a family of novel manganese-selective cation diffusion facilitators
in C. elegans. Although these are not essential proteins and are unlikely to be good drug targets,
their newly discovered role in manganese tolerance advances our knowledge of the mechanisms
that underlie manganese biology and toxicity.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2022-06-30
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Provider |
Vancouver : University of British Columbia Library
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
DOI |
10.14288/1.0402548
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URI | |
Degree | |
Program | |
Affiliation | |
Degree Grantor |
University of British Columbia
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Graduation Date |
2021-11
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Campus | |
Scholarly Level |
Graduate
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Rights URI | |
Aggregated Source Repository |
DSpace
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Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International