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Decontamination of rough implant surfaces in vitro using glycine powder air polishing

University of British Columbia

Objectives: The prevalence of peri-implantitis and subsequent failure of implants continues to rise. The majority of cases are bacterial in etiology. In peri-implantitis, the subsequent bone loss around dental implants exposes their micro-roughened surfaces to bacterial colonization, further aggravating inflammation, bone loss and progression of disease. The challenge of peri-implantitis treatment is the complete decontamination of roughened implant surfaces once colonized with a biofilm. Many chemical agents are not effective in decontamination of the rough-surface implants with mature biofilms. Therefore, we examined if air-flow with glycine (AFG) would be effective in decontamination of implants with mature subgingival mature biofilms. Methods: SLA® (sand-blasted acid-etched) titanium discs were inoculated with dental plaque and anaerobically incubated at 37°C for 21 days to allow for the formation of a structurally mature oral biofilm on the disc surface. Discs were then separated into different treatment groups: AFG with or without the powder and with or without prior rinsing with 0.9% sterile saline. Control groups included no saline rinse, single rinse and double rinse controls. For assessment of decontamination, discs were imaged, and bacterial cells counted, under a scanning electron microscope (SEM) at 5000X magnification. Results: The no-rinse control group contained multi-species undisrupted biofilm. Saline rinsing removed most of the biofilm except the rough-surface-associated microorganisms. Numerous cocci and some rods remaining on the pits of the SLA® surface. AFG without the powder was not effective in decontamination of the rough SLA® surface. However, AFG with the powder produced practically clean SLA® surfaces. In addition, prior rinsing with saline was not necessary for this effect. Conclusions: AFG appears be an effective method to decontaminate mature biofilm contaminated implant surfaces when used directly on the contaminated implant surface.

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2018-08-09

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/66716

Craniofacial Science

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/