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UBC Theses and Dissertations

A contribution to the embryological study of SQUALUS SUCKLII, Girard, with notes on the life-history Lucas, Verna Zora 1930

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U.B.C.  L B R A R Y  ^ CAT M O l ^ ^ ; . i ACC. MO ^ .a' . A CONTRIBUTION TO THE EMBRYOLOGICAL STUDY OF SQUALUS SUCKLII, GIRARD  WITH NOTES ON THE LIFE-HISTORY  by  Verna Zora Lucas  A Thesis Submitted for the Decree of I&ster of Arts In the Department of Zoology  The University of British Columbia April 1930.  INTRODUCTION  The study of the embryology ana life-history of Squalus sucklii, Girard, was commenced at the University of British Columbia in the winter of 1928, continued at the Pacific Biological Station at DEPARTURE Bay tHe summer of 1929 where some material was obtained from the reduction Plant in Nanaimo, And again continued at tHe University during the last session. A particular study is made of tHe embryology of a 21 mm. series, although, for sake of comparison, series were made of embryos of 10 to 40 mm. in length. The data concerning the life-history, particularly that on the period of gestation , were found tu be in part similar to tnat of Squalus acanthias and in Part dissimilar, the differences and similarities are discussed in conjunction with the considerations of the paper of E. Ford, 1921, from the Plymouth Biological Station. I shoula lik  to express m^ appreciation of the  assistance and suggestions given by Dr. C. McLean Fraser and Dr. A'.A. Clemens.  i4y thanks are also due ^r. I.J. Spencer  f(ir procuring literature and i'^i many su^j stions on techique.  CONTENTS  A  EMBRYOLOGICAL STUDY OF A 2.10 CI,:. EJBRYO  I  EXTERNAL FEATURES  II  NERVOUS SYSTEi: a.  Flexures  b.  Central nervous system  c.  III  1.  telencephalon  2.  diencephalon  3.  mesencephalon  4.  metencephalon  5.  myelencephalon  Spinal cord 1.  general structure  2.  nourocoele closures  d.  Spinal nerves  e.  Sympathetic nervous system  f.  Cranial nerves  ORGANS OF Sl-ECIAL SLI7SE a.  Nose  b.  Eye  c.  Ear  d.  Lateral line  - 11 IV  ALIMENTARY TRACT AND ITS APPENDAGES A.  b.  Alimentary tract 1.  pharynx and thyroid  2.  stomach  3.  duodenum  4.  valvular intestine  5+  colon and rectum  6.  cloaca  7.  postanal gut  Glands and Appendages 1.  liver and gall bladder  2.  pancreas  3.  subnotochordal rod  V  MESENTERIES  71  ACCESSORY GLANDS  711  a.  Interrenal  b.  Suprarenal  c.  Spleen  RESPIRATORY SYSTEM a.  Gill Slits  b.  Branchial arches  c.  Gill filaments  - Ill Vili  CIRCULATORY SYSTEM a.  Heart  b.  Arteries  c.  Veins  IX  NOTOCEORD  X  irUSCLE ILATEH  XI  URINO-GENITAL SYSTEM a.  Excretory system  b.  Genital system  B  LIFJ, HISTORY NOTES  I  PERIOD 0? GESTATION  II  COMPARISON OF SQUALUS SUCZLII AND SQUALUS ACARTrlAS.  EXTERNAL APPEARANCE.  Cm opening the egg-capsule the opaque, yellowish white embryo and the yolk sac are readily separated from the watery albumen.  The embryo is attached to the yolk sac by a  long yolk stalk which, even at an early stage of development, allows a good deal of movement.  The embryo is quite active  moving with the typical wave-like motion of the adult.  The  total length of the embryo is 2.10 cm., the head being twosevenths of the total length. The body is quite straight; the head large, the rest tapering evenly to the tail.  The mid-brain is the most  anterior portion with the fore-brain at an angle of ninety degrees with the main axis of the body.  The eyes are prom-  inent; the choroid fissure showing distinctly on the posterior margin.  The olfactory vesicles are simple pits.  The  first gill slit is distinctly differentiated into the spiracle having closed ventrally and moved to lie at an angle of forty-five degrees with the second gill slit.  The second  gill slit extends two-thirds of the distance across the side of the body; the remaining slits growing progressively smaller, the sixth being half as long as the second.  Cill fila-  ments show from all the gill slits; the greatest development is in the second where the filaments are 1.5 mm. in length, the least in the spiracle where there are six short knobs. The mouth remains as a triangular opening into the buccal  - 2 cavity ahowing an indication of the development of the slitlike adult mouth.  The middle lateral portion of each gill  arch has grown posteriorly over the next to form the gill pouch or pocket. The rudiments of the fins are well developed giving indication of the unpaired dorsal, second dorsal and caudal, the paired pectorals and pelvics.  The dorsal and  second dorsal, however, show no indication of the anterior fin spines.  NERVOUS SYSTEM  The most noticoable fact about the brain is the extreme flexion that it has undergone.  The fore and mid-  brain are literally curved over the end of the notochord. The flexure starts in the mid-brain which results in bringing this region into a position approximately anterior to the end of the notochord.  The movement at the same time  brings the fore-brain directly under the notochord with its original anterior end facing postero-ventrally. Constrictions have divided the brain into forebrain, mid-brain and hind-brain.  The foro-brain has divided  into the telencephalon and diencephalon, the hind-brain into the metencephalon and the myelencophalon.  Tho mid-brain re-  mains single as the nesenccphalcn. The telencephalon is large, far larger than the posterior portion of the prosencephalon, the diencephalon. The lateral portions which are adjacent to tho thin wall of the dorsal cleft dividing thetel- and diencephalon are slightly folded around the smaller diencephalon.  The optic  stalks are given off laterally froc the vide canal connecting the tel- and diencephalon.  Separating the anterior part  of the telencephalon from this portion are slight vontro-  - 4 -  lateral constrictions. The diencephalon is separated dorsally from the telencephalon by a deep, wide, thin-walled cleft, and ventrally by a slight constriction.  The anterior wall of the  diencephalon is very thin and flattened, the laterals walls thickened.  It is connected posteriorly with the telencepha-  lon by a wide canal and separated from the mesencephalon by lateral constrictions.  Posterior to the above mentioned  cleft and in the roof of the diencephalon the epiphysis arises as a long, simple tube tapering to the tip.  On the  floor dorsal to the optic stalkb the infundibulum is developed as a round evagination having thin walls and a slightly constricted base. The mesencephalon is more distinctly marked off from the fore-brain than from the hind.  The lateral walls  are thicker than the roof and floor, the cavity elliptical in shape. The mes- and motencophalon are joined by a wide channel.  The middorsal line of the metencephalomis thin  walled; the lateral walls thickened in four places to form distinct horns which project out into the ventricle, the dorso-lateral projections, the alar plate, being greater than the ventro-lateral basal plt.te.  The myelencephalon has a very thin roof extending laterally to the median line in its greatest development. It looks just as if the walls had been stretched apart at the dorsal line to form a wile open V leaving a very thin strip of nervous tissue across the top.  The lateral walls  are quite thick and close in on one another dorsally as the myelencephalon tapers towards the spinal cord.  The basal  plate projects farther into the neurocoele, the alar plate less than in the metencephalon, thus forming midventrally a deep sulcus around which the ependymal layer is more evident than in the lateral walls. The spinal cord extends from the myelencephalon to the tail tapering to end just anterior to the neurenteric eancl.  In cross-section it is slightly ovoid, being larger  ventrally, with the three primary layers around a very narrow, laterally compressed neurocoele.  The innermost layer,  the ependymal, which encircles the whole neurocoele is widest laterally where it is half the width of the cord.  The  middle or mantle layer is composed of two crescent-shaped parts lying on either side of the cord which are only connected by a commissure marginal layer. of nerve fibres.  ventrally.  The outer layer is the  It is free of nuclei and composed entirely This layer is divided into two portions:  - 6an inner densely fibrous one and an outer vesicular part crossed by fibres from the inner nucleated layers. A very interesting fact concerning the lumen of the spinal cord was noticed and investigated, namely that the walls of the neural tube during a certain period of embryonic development approximated and fused to give two or three lumina.  This condition was traced thru a series of  embryos which were obtained in different years and at different periods of the year, preserved in different typos of fixers and imbedded under different conditions.  Because  of this variation it was concluded that the condition was not one due to physiological or histological factors but was purely an embryonic structure.  Embryos 1.02 cm. in  length showed no evidence of the closures while those of 1.15 cm., 1.29 cm., 1.62 cm., 2.15 cm., and 2.97 cm. showed a gradual development thru the series. and 3.49 em. had no trace of them.  Embryos of 3.20 cm.  From these facts it was  concluded' to be of a transitory nature. The following is a description of the condition in the embryo being studied. Opposite the second gill slit the first indication of this closure is seen.  It seems to be due to the  growth of the cells in the basal plate described above. Those plates grow towards one another, fuse and thus close  - 7 over the ventral sulcus of the neural tube.  This sulcus re-  mains thin walled as does the dorsal one which is seen in the spinal cord where it joins the medulla.  Posteriorly the gen-  eral shape of the lumen becomes more rectangular, the sides more parallel as they approach one another.  Opposite the  anterior part of the third gill slit there is an indication of the enlargement of the two alar plates closing in slightly ventral to the middle of the remaining lumen.  At the  posterior part of the third gill slit these plates come together and fuse.  Thus are formed the three lumina of the  spinal cord seen in cross section.  The dorsal lumen is  triangular in shape, larger than the other two.  The central  one is long and narrow; the middle one slightly shorter but more rounded than the ventral.  The two points of fusion  differ in that the dorsal one is about three times as long as the ventral.  Three sections (36 micra) anterior to the  fourth gill slit the ventral fusion disappears and the resulting ventral tube becomes long and narrow with a dilated dorsal portion which was the middle lumen.  Meanwhile the  ventral lumen has become smaller and round assuming the shape of a long inverted drop. and more round.  The spinal cord grows smaller  At the sixth gill slit the cord is oval,  the dorsal lumen quite small; the large ventral lumen elliptical, its dorso-ventral length slightly less than half the  - 8 dorso-ventral length of the cord. Opposite the yolk stalk where the body cavity becomes continuous with that of the stalk the dorsal fusion disappears and the neural tube assumes a longer elliptical outline than the ventral portion had.  Just opposite the  posterior border of the yolk stalk the ventral fusion again appears.  Four sections (48 micra) posterior to this the  ventral one reappears.  Two sections (24 micra) posterior  to this the middle lumen closes leaving a dorsal and a ventral lumen, the ventral lumen being twice as large as the dorsal. the cord.  This fusion extends over half the total length of From here the tube remains in this condition until  the neurenteric canal is reached where the fusion disappears and the neural canal assumes a spherical outline. The spinal nerves consist of a dorsal ganglionated and a ventral non-ganglionated root.  These roots unite a  short distance from the cord, then divide sending off a lateral and slightly dorsal nerve to the muscle plate, the dorsal ramus, a median ventral nerve to the segmental sympathetic ganglion, the ramus communicans, and a ventral norve to the body wall, the ventral ramus.  At the point where the  ramus communicans enters the sympathetic ganglion a ventral nerve is given off the ganglion which rune parallel to the ventral ramuB out thru the lateral muscle plate and vent-  - 9 rally along its outer border.  Branches of both the sympath-  etic and ventral rami are given off to the pectoral and pelvie fin buds.  Opposite the yolk-stalk the sympathetic nerve  branches just before passing thru the muscle plate, and sends a nerve ventrally between the muscle plate and the wall of the coelom. The primary sympathetic ganglia lie ventro-laterally from the notochord in the centre of a triangle bounded by the dorsal aorta, the pronephric duct and the dorsal muscle plate.  In a few sections small masses of cells have  formed dorso-median to the primary sympathetic ganglia. These are the secondary sympathetic ganglia which are connected to the primary ganglia by a commissure. Only five of the ten cranial nerves are developed, namely the fifth or trigeminal, the seventh or facial, the eighth or auditory, the ninth or glossopharyngeal and the tenth or vagus. In a section thru the ventral portion of the optic stalk and the mid portion of the infundibulum the origin of the fifth cranial nerve is seen.  It arises from the medulla  by two distinct roots and enters a large ganglion, the Gasserian ganglion.  On emerging from this ganglion it di-  vides into two parts, an anterior one going to lie beside the  - 10 optic cup — t h e opthalmicus profundus nerve—, and a posterior o n e — the mandibular nerve.  The mandibular nerve then  branches into a small dorsal nerve and a large ventral nerve. Both parts run ventro-posteriorly into the mandibular arch. The anterior branch runs to the ventral wall of the arch, the posterior one lies close beside the muscle plate in the arch and curves ventro-medially when the arch closes under the mouth.  The anterior branch is the maxillary division  of the trigeminal, the posterior one the mandibular division, supplying the upper and lower jaws respectively. In a section thru the tip of the diencephalon and the optic vesicle the root of the seventh and oighth cranial nerves is seen.  These two nerves are very closely associated  arising as they do from a single large ganglion.  This root  passes into a large ganglion from which is sent anterior a large nerve, the palatine branch of the seventh nerve.  This  nerve runs ventro-anteriorly near the skin in the angle made by the optic vesicle and the hind brain to supply the region of the palate.  After the palatine is given off the main  stem runs posteriorly giving off a small dorso-lateral branch to the skin which will eventually supply the superficial constrictor muscles.  The main part then passes out  to the junction of the upper and lower jaws where it divides into an anterior branch which runs Immediately below the  - 11 skin towards the angle of the jaw, and a posterior branch which passes along the posterior margin of the hyoid arch. From the combined three posterior branches of the facial the eighth or auditory nerve is given off opposite the anterior end of the auditory vesicle.  This nerve enters  a large ganglion which lies close beside the auditory vesicle.  From the ganglion nerve fibres are sent out to the  semicircular canal rudiments. Opposite the posterior part of the ear the ninth or glossopharyngeal nerve rises from the medulla oblongata. Passing posterolatero-ventrally it dips ventral to the auditory vesicle where it enters a large ganglion.  From this  ganglion a small branch is given off dorpo-latorally to the lateral line.  The main branch then goes to the outer and  anterior border of the hyoid arch, enters a ganglion and sends off a small branch to the anterior dorsal wall of the arch — a branchial nervous supply for the external gills. After sending off this branch the main stem passes ventrally to lie beside the muscle plate in thp arch. In an earlier stage the vagus arose as a series of four roots from the posterior portion of the medulla.  Grad-  ually the posterior roots were lost and now only the two anterior ones remain.  The two posterior ones arise as  branches of the first and second root ganglion.  - 12 At the extreme posterior border of the auditory vesicle the first root of the vagus is given off the myelencephalon. is given off. ganglion.  A short distance posterior the second root  Shortly after leaving the cord each enters a  From these ganglia each sends off two branches,  a small anterior one to the lateral line, the other to enter a large commissure.  This commissure rises from the first  root, passes directly posterior receiving the branch of the second root and continues posteriorly over the remaining arches to end when the intestinal nerve of the vagus is given off.  From this commissure into the third, fourth, fifth  and sixth arches and the posterior wall of the sixth gill slit nerves are sent ventro-laterally to the outer, anterior border of the third arch where a small branch goes to the gill filaments.  Just anterior to the places where the last  three above mentioned nerves are given off small nerves arise and pass dorso-laterally to lie in the groove of the lateral line.  The main commissure then continues ventrally  and posteriorly to become the Intestinal branch of the vagus ending in an enlarged ganglion mass lateral to the oesophagus. The roots of the glossopharyngeal and the vagus are connected close to the medulla by a commissure which is continuous with the dorsal roots of the spinal nerves.  It  - 13 is oJ the same cellular structure as the more ventral commissure although of much smaller size.  ORGANS OF SPECIAL SENSE  Under the organs of special sense are described the olfactory organ, the eye, the ear and the lateral line. The olfactory organs are simple somewhat triangular pits opening to the outside by a wide circular mouth. There are no indications of Schneiderian folds. Some parts of the eye are well developed, others indicated so that a general conception is reached as to the nature of the adult structure.  In the optic cup the inner  retinal layer is thickened and closely appliod to the outer narrow pigmented layer.  A concentration of mesenchyme about  the cup indicates the future development of the choroid and sclerotic coats. The lens is large and ovoid, alightly flattened on the median side. side is extensive.  The growth of cells from this flattened They have grown out radially towards the  outer wall nearly closing the lens vesicle*  The nuclei of  this portion are large and faintly staining with few gran-  - 14 ules.  The outer wall, the lens epithelium, is two oells  deep, the nuclei being at different levels and darkly staining. The short, broad optic stalk is attached to the ventral proximal portion of the cup at the base of the choroid fissure and shows at this point a slight indication of the infolding to form a tube down which the optic nerve fibres from the retina will ultimately grow. Projecting into the optic cup thru the choroid fissure is a mesodermal structure the processus falciformis which spreads out over the retina to form a flattened disc. From the upper surface of the disc long slightly intertwining fibres radiate out into the posterior chamber.  A  small blood vessel enters the processus falciformis at the choroid fissure and branches into the jileate structure. In the ear the three semicircular canal rudiments are quite evident extending as pockets from the otocyst proper.  The anterior oblique canal projects antero-dorsally,  the posterior oblique postero-laterally and the horizontal laterally towards the skin.  The sacculus is enlarged ven-  trally and tapers dorsally into the endolymphatic duct which opens to the exterior postero-dorsally to the posterior extremity of the posterior oblique canal.  In the thiekened  - 15 ventral walls of the canal rudiments the ampullae appear as groups of large clear cells.  To the base of etch cell is  attached a long nervous fibre which is connected to the auditory nerve. The lateral line has developed anteriorly to the third gill arch and posteriorly to a position opposite the rectal gland.  It is formed by a thickening of the ectoderm  at the level of the notochord which gives rise to an inner layer, the subsequent mucous layer of the epidermis.  The  nervous supply of this organ is furnished by branches from the vagus and glossopharyngeal nerves which coKe to lie in a hollow in the mucous layer.  ALIMENTARY TRACT AND ITS APPENDAGES  The mouth or entrance to the buccal cavity is still widely open although assuming the slit-like appearance of the adult.  There is no constriction between the buccal cavity  and the pharynx.  The pharynx extends laterally into the  spiracle and gill slits. The thyroid has lost all connection with the pharynx and lies in the floor of the pharynx opposite the  - 16 third gill slit.  In shape it is rod-like, triangular in  the centre and pointed at both ends. The pharynx before joining the oesophagus is flattened dorso-ventrally in a wide curve.  The oesophagus  is small and entirely closed having no lumen whatsoever. Opposite the posterior portion of the ventricle the oesophagus enters the stomach.  This is a crescent-shaped laterally  flattened structure, curving first to the left side then ventro-medially to enter the intestine.  The larger left  limb is the cardiac, the median smaller the pyloric division. The first part of the intestine, the duodenum is short though well defined.  Into this segment the ducts of  the liver and pancreas and the yolk stalk enter.  The walls  are thin and where the yolk-stalk enters are thrown into irregular folds. The main portion of the intestine is taken up by the spiral valve.  It is seen to be formed by an ingrowth  of the intestinal wall to form a series of annular folds eleven in number, the posterior ones being closer together than the anterior. The portions of the intestine succeeding the valvular intestine, the colon and rectum, are differentiated by general structure and also by the fact that the rect-  - 17 al gland empties into the junction of these two parts.  The  walls of the colon are thicker, the cells more columnar than those of the rectum.  On the other hand the lumen of  the former is small and round while that of the latter is crescent-shaped.  The rectal gland is a simple antero-dorso-  lateral outpocketing of the wall of the intestine at the junction of the colon and rectum. The rectum leads into an enlarged space, the cloaca, which forms,a eonron receptacle for the digestive and urinary, and later the genital systems.  It is somewhat  flattened laterally and ends blindly after the walls of the segmental ducts have fused with its walls.  The procto-  daeum is not yet broken thru, there beim? a close application but no fusion between the ectoderm and endodermal layers. The posterior portion of the gat which in the earlier stages is called the postanal gut has completely atrophied anteriorly and is only seen in the very tail region where it curves about the posterior portion of the notochord to join the neural tube and becomes the nourenteric canal.  - 18 -  GLANDS.  Under this heading are discussed those parts which are either derivatives of the alimentary tract or are associated with it in the adult. The main portion of the liver arises as a mass of embryonic liver tissue in that region bounded anteriorly by the sinus venosus and posteriorly by the yolk stalk. From this portion three lobes push posteriorly, a long right and left, and a small median caudate.  The gall bladd-  er is situated in the caudate lobe, is drained by a duct which, joined by the other ducts from the lobes of the liver, forms the ductus choleduchus and empties into the proximal portion of the duodenum on the dorsal side.  Posteriorly the  right and left lobes of the liver (the left being the larger) extend opposite the opening of the pancreatic duet into the intestine. The pancreas is composed of two lobes, a central portion connecting these and a duct.  The dorsal lobe is  large and expanded into a number of diverticula.  From these  the central portion leads latero-ventrally to meet the more compact ventral lobe which has a larger number of less branched diverticula.  From the ventral lobe the short pan-  creatic duet leads into the duodenum just anterior to the  - 19 first fold of the valvular intestine. The subnotochordal rod underlies the whole length of the notochord except for the small curved anterior portion. Anteriorly it is very small ana shows signs of atrophy.  Cen-  trally it shows its highest stage of development as a small mass of darkly staining cells.  In the region of the neuren-  teric canal it is quite rudimentary and shows its origin as a proliferation,of the cells of the dorsal wal3 of the ?ut.  I^SENTERIES  The mesenteries and septa which are to form partitions between the different body cavities are being developed.  Around the sinus venosus mesenchyme is collecting to  form the rudiment of the lateral mesocardium.  The single  dorsal mesentery which in an earlier stage supported the whole of the digestive tract is beginning to break down to form the adult structure—a large anterior and a small posterior mesentery.  This is accomplished by holes appearing  in the mesentery; these coalesce and the mesentery disappears. The posterior mesentery extends from the anterior tip of the rectal gland posteriorly.  The anterior nesentory extends ov-  er the greater part of the remaining portion ending a short distance anterior to the rectal gland.  Just before the yolk  - 20 stalk enters the body a septum forms and attaches the liver to the ventral body wall.  This is, however, the only r  whore the two lateral sides of tie body cavity ;ro completely separated from one another.  ACCESSORY GLANDS  The interrenal gland is an unpaired rod-like mass of cells lying between the dorsal aorta and caudal vein in the region of the posterior end of the kidney.  Ac yet the  cell mass is undifferentiated except that there is a slight indication of a fine membraneous covering. The sympathetic ganglia r^hich forn; the basis of tbo suprarenal glands are well developed and have oonspicuous branches connecting them with the spinal nerves.  Branches  which are masses of dark staining cells with a definite lumen are given off to the 'lorsal aorta.  The giii^lia them-  selves show an indication of division into a ventral suprarenal and a dorsal sympathetic portion. The rudiment of the spleon is seen as a mass of an -differentiated colls derived from a proliferation of mesenchymal cells.  This projects out into the body ctvit^ from  the left aide of the dorsal ¡.osentery opposite the pancicas.  - 21 -  RESPIRATORY SYSTEM  The respiratory tract comprises the mouth, pharynx, gill slits and gill filaments. The spiracle, the most anterior of the six gill slits, hus become definitely differentiated, and lies at an angle of forty-five degrees with the long axis of '-he body* The second gill slit lies slightly at an anr?lo with the dorsal portion pointing anteriorly.  The fifth and sixth slits arc  at right angles to the body axis. The branchial arches have lengthened out laterally, the central part becoming a plate, the septum from which the gill filaments arise; the distal pert folding over the next posterior arch. The embryonic gill filaments arise as nodules from the septum of the walls of the gill slits.  Bach nodule  lengthens out and forms a more or less flattened plate about whose outer border a blood vessel circles.  -'ilanents have  developed on the anterior walls of the spiracle and the sixth gill slit and on both anterior and posterior walls of the four remaining arches.  The filaments from the anterior walls are  relatively much longer than those on the posterior walls.  - 22 CIRCULATORY SYSTEM  HEART  The heart consists of a large thin-walled auricle lying dorsal to a smaller thick-walled ventricle; anteriorly from the ventricle is a conus arteriosus, posteriorly from the auricle a sinus venosus. Indications of valves show in the conus arteriosus and between the auricle and ventricle.  Those in the conus  arteriosus appear as three triangular folds, one dorsal, two latero-vontrai, projecting into the lumen of the conus; those between the auricle and ventricle as two folds pointing anteriorly into the auricle.  ARTERIES  The conus arteriosus leads anteriorly into the ventral aorta which gives off the six afferent aortic arches to the branchial archos.  The fifth and sixth afferents  leave the ventral aorta together dividing shortly after; the rest come off separately.  These traverse the outer posterior  walls of the branchial arches, send off branches to the gill filaments and run dorsally to join the dorsal aorta.  A  - 23 secondary arch is developed in the gill filament region* consists of two branches.  It  The posterior branch arises in  the filament region in the posterior wall of the branchial arch, sends three vessels directly anterior to meet the anterior branch in the filament region in the anterior wall of the branchial arch.  These are the efferent collectors  which, joinod by the afferent collectors enter the dorsal aorta. The dorsal aorta is divided for the greater part of the arch system.  It branches immediately to the entrance  of the sixth efferent arch and unites again just anterior to the spiraeular cleft so that all ?:he arches but the sixth efferent empty into separate right and left dorsal aortae. The single dorsal aorta continues anteriorly to the baso of the infundibulum where it again divides sending branches to lie close beside the wall of the mesencephalon.  Immediately  posterior to this division branches enter from the mandibular regions.  These are the pseudobranchial arteries.  They  arise from the posterior portion of tho first afferent collector, run anteriorly under the spiracle, then dorso-anteriorly to join the dorsal aorta.  Posterior to the aortic  arches the dorsal aorta continues as an undivided vessel to the tail region T.hero it beoomcs the caudal artery.  The one  large branch given off opposite to the posterior border of  - 24 the ventricle is the omphalomesenteric or vitelline artery* It dips ventrally and to the right side to enter the yolk stalk. ment  Paired branches are given off the aorta to every segsegmental arteries.  VEINS.  Blood is collected from the head region by the anterior cardinal veins which receive vessels from about the di- and mesencephalon and the optic vesicles.  Continuing  posteriorly they swerve laterally and come to lie ventrolaterally from the auditory vesicles near the skin.  In the  branchial region they lie lateral to the dorsal aorta.  In  the heart region they dip ventro-laterally to enter the common cardinal veins. Blood is collected in the tail region by the caudal vein immediately ventral in position to the caudal artery. Opposite the anterior end of the rectal gland this vein divides to form the two postcardinals.  These continue an-  teriorly on the median side of the kidney and enter the common cardinal veins at the same time as the anterior crrdinals. The hepatic veins run alon<? the lateral walls the  - 25 entire length of the liver lobes and enter separately into the posterior part of the sinus venosus.  The omphalomesen-  teric or vitelline vein enters the central portion of the liver and gives off two vessels which run along the median walls of the left and right liver lobes.  NOTOCIiORD  A section cut at rigbt angles to the longitudinal axis of the body and passing thru the infundibulum also passes thru the anterior end of the notochord*  In cross  section the notcchord consists of large vacuolate cells the nuclei of which are concentrated at the outer margin, and a definite non-cellular sheath.  In the oesophageal legion  the notochord becomes flattened on the ventral side.  Oppo-  site the pancreas it is as large as the neural tube.  From  this point posteriorly it tapers off hoeping the same sise as the neural tube and ends at the beginning of the neurenteric canal.  - 26 -  MUSCLE PLATES  The muscle plates are developed from the somites which form the segmental plates of the body.  Each somite  has divided into a dorsal segment and a ventral segment. The dorsal segment consists of an outer layer, the dermatome, and an inner, the myotome.  The dermatome has again divided  into an outer layer of cells with small nuclei a.-.d an inner layer of closely packed cells of larger nuclei.  From the  inner and ventral edges of the myotome loose sclerotomal cells have proliferated and formed a triangular mass opposite the upper part of the notochord.  Within the  yotome  muscle fibrillae have formed which give the myotome a white fibrous appearance. of the back.  These will ultimately form the muscles  From the outer ventral edge of the myotome  and from the ventral edge of the dermatome outgrowths extend ventrally close to the ectodermal wall.  These outgrowths  fuse and f *<rm the ventral plate. The ventral segment of the somite forms the lateral plate which lies close beside the wall of the coelom.  -  27 -  URIHO-SENITAL SYSTEM  EXCRETORY SYSTEM The urinary system consists of a pronephric or segmental duct and segmental tubules.  The pronephric duct  lies in the genital ridge and extends from the cloaca anteriorly to the origin of the omphalomesenteric artery from the dorsal aorta.  At its anterior end it is open to the  body cavity, at its posterior end it has a slight swelling which is the rudiment of the urinary bladder.  The walls of  this vesicle are cloaaly applied to the dorsal wall of the cloaca. The segmental tubes and nephrostomes appear more posteriorly, near the entrance of the bile duct into the duodenum.  Each segmental tubule consists of a nephrostome  opening to the body cavity.  The segmental duct grows dorso-  laterally from the nephrostome and dilates into the median vesicle which later forms the tissues for Bowman's capsules. The short renal tubule leaves the median ventral portion of the vesicle and empties into the la:ger primitive collecting tub .lo.  The total number of segmental tubules present  at this stage of development is thirty-one with the indication of two more at the anterior end.  - 28 -  GENITAL SYSTEM  On either side of the dorsal mesentery the eoelomic epithelial cells have become columnar and formed the rudiments of the two gonads.  In each segment groups of  primordial germs cells appear as large clear cells in the germinal epithelium.  In the highest stage of development  these cells have begun to wander in to the --onad rudiment, there to divide and multiply.  The secondary groups so  formed are bounded by cells of mesenchyme* There is no trace of the horizontally splitting of the pronephric duct to form the Kiillerlan duct.  - 29 LIFE-HISTORY NOTES  PERIOD OF (STATION.  General collections of embryos were made in the summer of 1929 fror Hay 15 to August 1, and at the beginning of the year, 1Ç30, from January 1 to .arch 15.  It was found  that small germinal discs were obtained over a period of three months namely, January, February and J arch.  There is  the possibility that this period may be as long- as that of Squalus acanthias recorded by ¡¡. Ford, page 503, who says that "newly formed embryos were obtained from November until the middle of Hay." At  same time, January to larch, embryos of 16  to 17 cm. were also obtained.  Similarly emiryos of two  sizes were obtained in the summer collecting-, the smaller ranging from 2.0 to 4.0 cm., the larger from 17.0 to 22.0 cm.  In the fall of 1914 Dr. C. LcLean Fraser took some  measurements of embryos.  V-ith these results to bridge over  the winter months it was seen that the period of gestation extends over a space of about twenty-three to twenty-four months.  This is illustrated in the graph of flate 1.  - 30 -  COMPARISON OF SQUALUS SUC,<'LII APD S(,UALUL! ACANTHIAS  In general these observations coincide with those of E. Ford on the life-history of Squalus acanthias but there are some points of difference.  I shall give below the  parts of the summary of his paper which deal with the early stages of development and shall discuss each part as to the differences and similarities of the two species. "2.  The female, before becoming sexually mature, under-  goes an extended adolescent period during which the initial set of ovarian eg^s are maturing." It was noticed that in some females bearing embryos of the second year cycle there wore maturing ovarian eggs which measured 1 to 1. inches in diameter in January and  to  inches in Kay.  This shows that as the embryos  are developing in the oviduct there are o g s maturing in the ovaries at such a rate as to be rea^y for fertilisation at the beginning of the thirr? year cycle. "0.  In a pregnant female the embryos arc of the same  general size, and similarly the ovarian eggs.'' "4.  Kales and females are equally represented in the  embryos end may occur together in the same uterus; they do not differ in size at any corresponding stage of embryonic  - SI development." A typical example of this is shewn in the embryos from a female caught on June 22, 1929. Length of embryo 21.6 cm.  Sex ?  21.6  "  21.6  "  tf  20.9  "  (f  21.6 em. 21.6  "  20.3  "  20. r  "  20.S  "  right oviduct  ? ?  left oviduct  ?  This also shows that, as the total volume of the two oviducts are almost the same, that oviduct which has the fewer number of oubryos will iiave larger ones than the other oviduct with more embryos. "5.  The number of embryos carriod by one fish, if the  length of the parent is ignored, is most frequently from throe to four in an observed range from one to eleven*  It  may be possible, however, that the number of embryos is dependent on the length of the parent, for the largest number of embryos was found in the largest fish."  - 32 In Squalus sucklii the observed average is nine to ten in the largest fish and five to six in the smaller, the range being five to twelve.  In fact there were no  females caught in which there were less than five embryos or five developing ovarian eggs. "6.  The remains of the egg-capsule in which tbe  earlier developmental stages are undergone may continue in the uterus until the embryos are ready for birth." There has' never been observed any remains of the egg-capsule in the oviducts of S. sucklii after it has become ruptured.  This may be explained that, because of the  thin membraneous structure cf the capsule, it may be absorbed or it may pass oat of the uterus more easily than can the hard horny one of S. acanthias. Ford does noteive the time of the cycle when the egg-capsule is ruptured leaving the developing embryos free in the oviduct.  This evidently occurs sometime in the fall  of the first year cycle as embryos in August have a capsule while those in November hive none. It has been noted by Balfour that Elasmobranch eggs pocsess no membrane other than a surfaco tension one at the interfaces of the yolk and albumen.  It may be that the  egg-capsule is used to form a protecting surface about these.  As the blastoderm grows it spreads over the yolk  - 33 forming a yolk membrane which eventually surrounds the whole yolk.  When this stage is reached the egg-capsule is  no longer required and it is perhaps then that it is passed off allowing more space and freer movement for the developing embryos. "8.  Newly formed embryos were obtained from November  until the middle cf Hay." This has been discussed above* "12.  The constitution of the shoale of adult fishes is  governed by the factors of sise and sexual condition. As all observations wore made on fish caught by set lines only tho larger fish were observed, but it was noticed that among these estcbes there was a definite segregation according to size and sex. into the Reduction "Plant at Nanaimo, B.  One scow-load brought consisted of two  catches from the mouth of the Praser River, about seventy percent of which were large mature males, the other thirty percent made up evenly of immature males and f riles of the same size.  The same day, Hay 20, 1929, a catch from Snake  Island near Nanaimo from shallow water consisted of large females carrying embryos.  Another catch brought in pregnant  fomaTes and a small amount of immature rales and females. This tends to show that the im ature males and females have e wider range of occurence, the mature males and pregnant  - 34 -  females keeping in definite shoals  UBC Scanned by UBC Library  - 35 -  LITERATURE  Arey, Leslie Brainerd. ers Company. Balfour, F. M.  Philadelphia and London  1924.  8°: 1-295, 9 pis., 9 text figs.  Lon-  1878.  Balfour, F. K.  On the origin and history of the urino^enitel  organs of vertebrates. Beard, J.  R. B. Saund-  A Monograph on the Development of Elasmo-  braneh Fishes. don  Developmental Anatomy.  Journ. Anat. Phys. 10:17  1876.  On the development of the common skate (Raja  batis).  Fishery Board for Scotland,  Beer, C. R. de.  The segmentation of the head in Squalus  acanthias. 13 figs. Coles, E. M.  (,uart. Journ. Licrosc. Sc. London.  66: 457-474,  1922.  The segmental arteries of Squalus sucklii.  Univ. Calif. Pub. Xool. Daniel, Frank J. Press.  1889.  SlJ: 03-110.  The Ulasmobranch Fishes.  Berkeley.  Eigenmann, Carl H.  1928. Univ. of Calif.  1928.  On the Viviparous Fishes of the Pacific  Coast of North America. 12:381-478, plates.  Bull, of U.S. Fish Comm.  1892.  — 36 —  Fee, A. R.  The Histology of the colon and its contained  spiral valve of the lacifie Coast Dog-fish (Squalus sucklii), with an investigation of the phylogeny of the intestinal valve. Ottawa. Ford, E.  Proe. Trans. Hoy. Soc. Can.  19: 169-195, pis.  1925.  A Contribution to our Knowledge of the Life-Hist-  ories of the Do Jishes landed at Plymouth. Biol. Ass. Plymouth. Fraser, C. McLean.  12: 469-505.  J. Har.  1921.  Ichthyological Notes.  Contributions to  Canadian Biology, being Studies from the Biological Stations of Canada, New Series. 1: 285-295. Goodrich, E. S. Black.  A Treatise on Zoology.  London  Griffin, Lawrence E.  A Guide for the Dissection of the Portland,  A Contribution Towards the Life-History of  the Spur-Dog.  J o u m . Mar. Biol. Ass.  17": 529-576, 16 text figs.  Jenkinson, J. W. Press.  Reed College.  1922.  Sickling, C. F.  B. S.  Adam and Charles  1909.  Dogfish (Squalus acanthias). Oregon.  1923.  Vertebrate Embryology.  Oxford,  1913.  Plymouth.  1930. At the Clarendon  - 37 Jordan, David Starr.  A Classification of Fishes including  Families and Genera as Far as Known. Publ.  (Biol. Sei.)  3".  Stanford Univ.  1923.  Jordan, David Starr, and Barton barren Evermann. of North America.  Smithsonian Institute.  The Fishes Vol. 1-4.  1900. Kellicott, William E.  Outlines of Chordate Development.  Henry Holt and Company. McEwen, Robert S. Company.  New York.  Vertebrate Embryology.  New York.  1927. Henry Holt and  1923.  Mcintosh, W. 0. and E. B. Prince.  On the Development and  Life-Histories of the Teleostean Food and other Fishes. Trans. Royal. Soc. Edinburgh.  35": 1-665, pis. 1-28.  1890. Meek, A. The encephalomeres and cranial nerves of an embryo of Acanthias vulgaris.  Anat. Anz. Bd. 34: 473.  Parker, T. Jeffrey, and Killiam A. Haswell. Zoology. Sedgwick, A.  McMillan and Co.  A Text-Book of 1910.  Notes on Elasmobranch Development.  Journ. Ificr. Sei. 1892.  London.  London.  1909.  Quart.  (2), 33: 559-586, pi. 35.  - 38 SiHHaway, Waldo. Sons, Inc. Thompson J. 8.  Vertebrate Embryology. New York.  John Wiley ana  1937,  The Morphology of the Prosencephalon of  Spinax as a Type of Elasmobranch Fore-brain. burgh Tr. R. Soc. Waters, Bertram H. Brain. 1892,  52: 487-500, 2 pis.  Edin-  1919.  Primitive Segmentation of the Vertebrate  Quart. Journ. Hicr. Soc.  33: 457-476. pi.  PLATE I.  Cross-section of medulla oblongata Fusion of basal plate - b. Fusion of a and b  PLATE HT  - T h i n roof of the medulla .Jlpendymal _ Marttle  layer  layer  PLATE II.  Figure 1.  -  , eopening of b  Figure 2.  -  Fusion of b for the second time  Figure 3.  -  Closing of 1 by tne lengthening of a  Figure 4.  -  Same as 3. Phis condition continues posteriorly for some distance  Figure 5.  -  Same as 3.  r  PLATE n  p íí*  ¡i J3  Sameat.fïçi.3  Fi§5  J  PLATE III.  Figure 1. --  Reopening of both a and b  Figure 2. --  Fusion of a for second time  Figure 3. --  Fusion for third time of b  Figure 4. --  Complete closure of 2  fhe condition found in Figs. 1*2, and 3 is of very ahort duration being found in three consecutive sections Figure 5.  -  tne tail region  Figure 6.  -  Same as 5.  Figure 7.  -  Junction of the neural tube and the postanal gut to form the neurenteric canal  PLATE HT  3 ^  ! n iha  ttgion  Rg.6 -J\ieurentertc  carta!  PLATE IV.  Figure 1.  -  Reconstructions of segmental tubule from cross-sections  Figure 2.  -  Reconstruction of segmental tubule from longitudinal sections  Figure 3.  -  Reconstruction of brain  PLATE HT Primítiwcollcctmglühule _ Renal  tubule  flcdion vesicle Segmental duct Segmental tubule  —Primitive collecting tubule — R e n a l "tubule — M e d ¡ a n ve9icle _ Segmental tubule _ Segmental duct _ Rrlloneum  Metèncephalon Epiphysis Optic cup telencephalon -Olfactory pits  Jnfundibulum  Medulla Root  .Root  of  nerve  of 7 * a n d  -Root of  inerve  .Root of 10"'nerve  Enerva  PLATE V.  Figure 1.  -  Reconstruction of 5th cranial nerve - trigeminal  Figure 2.  -  Reconstruction of 7th and 8th cranial nerves- facial and auditory  Figure 3.  -  Reconstruction of 9th and 10th cranial nerves - glossopharyngeal and vagus  . R o o t of 5 nerve -Gasserian ganglion .Mandibular branch -Opthatmic  branch.  Root of 5"* n erve Branch to cen9trictor muscle ^ A u d i t o r y n e r vied arch _Branch to hyo _Brartch to skin -Fhlatine branch  JRl f 3'"'ninierrvvz e _ oo oo tt o' f7"o ' dnd8 _^ .H R o o 1 o f 9 " a n d t bot of 10* Mwt i  PLATE VI.  Figure 1.  -  reconstruction of aortic arches  Figure 2.  -  Reconstruction of vascular system  Figure 3.  -  Valves of conus arteriosus  r  PLATE 51  AfRrent collector _ „Paired dorsal aorta Dorsal aorta Conus arteriosus _ Efferent Ml lector Paired ventral aorta Spiracle Rseudobranchial artery  Aortic arch . — A n t r a l aorta Anterior cardinal vein ._Dorsa) aorta Conus arteriosus .-\6ntrlcle  .Conns arteriosus  -Auricle Common cardinal vein 5inus venosus --Omphalomesenteric vein Omphalomesenteric artery Hepatic vein  H§-3  .Valves (R . Coetom  Dorsal aorta  Caudal Caudal  artery vein  J  PLATiJ VII.  Figure 1.  -  Section thru optic cup to show falciform process and infolding of optic stalk  Figure 2.  -  Falciform process  Figure 3.  -  Lens  J  PLATE Vili  Figure 1.  -  Section thru anterior portion of auditory vesicle  2.  -  Section thru posterior portion of auditory vesicle  Figure 3.  -  Structure of ampulla of auditory vesicle  Figure 4.  -  Nasal pit  Figure  P L A T E  T m  -Endolymphatic duct -Anterior oblique  canal  -Sacculus .Auditory  nerve  Anterior oblique canal  Horizontal canal Fbslérior oblique canal  -Ol&ctory pit -Telencephalon  PLAT^ IX.  Figure 1.  -  Reconstruction of spinal nerve  Figure 2.  -  Lateral line  UBC Scanned by UBC Library  PLATE HT Dorsal  root  Spinal ganglion Antral  root  Notochord Nerve of lateral  line  -Ramus  communicans  "Antral  ramus  Sympathetic fbslerlor  Ventral  ganglion  cardinal  muscle  vein  plate  Fin bud  flg.1  Nerve of Myotome Dermatome  Figa  lateral  line  PLATE X  Figyre 1.  -  Solid oesophagus  Figure  -  Structure of valve in spiral intestine  Figure 3.  -  Structure of pancreas  Figrre 4.  -  Section thru posterior end of liver  Figure 5.  -  Cross-section of thyroid  PLATE HT  Dorsal  mesentery valve  intestinal wall  .-fbrsal pancreas Ancreatic Antral  duct  pancreas  .Junction of yolk-stalk and  ---  duodenum  Embryonic trabecule Hepatic  Fig4.  ThyroiJ  sinusoid  PLATE XI.  Figure 1.  -  Primitive ova  Figure 2.  -  Fusion of segmental duct to wall of cloaca  Figure 3.  -  Budding of cells from postanal gut to form subnotochordal rod  PLATE HT  —Peritoneum Connecti've tlssue  Primitive Germina)  ova epithehum  Cotlecting tubute ^egmental duct  Ooaca  Fig. 2  Prochdaeum  .Notochord  rod Rstänal  gut  PLATE XII.  Showing that the period of gestation extends over a space of approximately 24 months.  PLATE  23E  *  ÔQUALU3  5UCKU), GtRARD  ?ER)OD or CtEST/mON  cm.  Jan.  fib  Mar  Apr  Hay  June  Juty  Aug 3ept  Oct  Nov  Dec.  


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