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UBC Theses and Dissertations

Pt.I. An investigation into the drug content of certain medicinal plants. Pt.II. The quantitative determination… Winter, Alice Greta 1927

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PART I ACC. NO. ? 7 ¿ '4 > AN INVESTIGATION INTO THE DRUG CONTENT OF CERTAIN MEDICINAL PLANTS PART II THE QÜANTITATIVE DETERMINATION OF ETHER IN ETHER, ALCOHOL AND WATER MIXTURES by ALICE GRETA WINTER A Bies is submitted for the Degree of MASTER OF ARTS In the Department of CHEMISTRY .UNIVERSITY OF BRITISH COLUMBIA April 19 TABLE Off contents EAST I AN INVESTIGATION OF THE DRUG CONTENT OF CERTAIN MEDICINAL PLANTS CEHERAL IBTRODUCTIOÎÏ HYDRASTIS CANADENSIS Introduction History and Uses Habitat and Range Description of Plant and Rootstook Growing Conditions Harvesting and Drying Discussion of Analysis Method of U»S.P* Method of Cordin and Prescott Results of Analysis Conclusion ATROPA BELLADONNA Introduction Description of Plant Growing Conditions Discussion of Analysis Hethod of U. S. P. Results and Conclusion TAJI& OF .COBTfflftrS . (Oont*d) PODOPHYLLUM PELTATUM Introduction Description of Plant (Jrowiag Conditions Ms cuss ion of Analysis Method of U.S.P» Results POPULUS TRICHOCARPA Introduction Growing Conditions Determination of Balsam in Buds Determination of Specific Gravity Saponification Value Acid and lister Values Conclusion PART I AM  IfTyESTIGATIQII HTTP THE MUG CTBTlglT 0? GBRTAIIi HEDICIITAI PLAIITS1. GSHgRAL IIITRQ HIP TI Oil 15x0 present work consists of a report on the analysis of certain drug plants gram at the Botanical Gardens of the University of British Columbia, plants investigated were Hydrastis canadensis* Atropa belladonna, Podophyllum peltatwa* and Populus Trichocarpa» ftse®« plants are all important commercially, as they are of value in medicine» Besides the results of the analyses, an effort lias been made in each case to set forth the possibilities of growing the herbs under cultivation on a commercial b&sl®» mm&sns  ctmmmis mmmmm. For the past twenty years a wide interest lias been shown in the motivation of Hydras tie canadensis,, or Golden Seal, as it is more conjaonly called* Two different facts are responsible for this, a growth in the demand, duo to an increasing knowledge of the usefulness of this drug in medicine, and a gradual depletion of the natural sources of supply. Shese two causes, as mi^ it be expected, have greatly increased the market price, which has risen from about twenty cents a pound in 1894 to from ten to sixteen dollars a pound at the present time» Such a marked change la price naturally turned the attention of many drug-collectors and others to the possibility of cultivating this herb. At the present time it is found that Hydrastis can bo very successfully grown under special conditions, and that it is possible to increase the altealoi&al content of the herb over that which it possesses in the wild state, thus rendering it more valuable, HISTOBY 1!TD U3BS The earliest settlers of America learned of the virtue of Golden Seal from the Indians, who used the roots as a medicine, and the yellow juice as a stain for their hands and faces» An infusion of the root In water was regarded as a specific for inflamed eyes and sore mouth, while the ground root was used as a tonic» JJany different names, most of them derived from its yellow color, wore applied, such as Indian dye, Indian tumeric, jaundice root, yellow puccoon, wild curcuma etc. Later it became generally known as Golden Seal, the name being derived from its yellow color and the peculiar seal-like scars left on the root by former annual stems. Around the year 174? a demand for this herb was created by the eclectic school of practitioners, which led to it becoming an article of commerce. In I860 it was made official by the Pharmacopoeia of the United States* and since then the demand has steadily increased. At present it is used as a remedy for certain digestive disorders,for catarrhal affections of the mucous membranes, in the latter Instance being administered both internally and locally, and as a bitter tonic analogous to calumba. HABITAT AHP RAUGB In the earlier years of its use, until about 1900» enough Golden Seal occurred naturally to supply the demand. The plant Is indigenous to Horth America, and is native from Minnesota to Hew York and Ontario, also south to Virginia and Missouri. Ohio, Indiana, Kentucky and Virginia have, perhaps, been the largest producers. It is found growing in patches, chiefly on hillsides affording natural drainage, and in woods which supply both shade and an abundance of leaf mold* As the roots of this herb are the parts required, many of the natural sources of supply have been gradually destroyed by the drug-collectors, who showed no discretion in their choice of season, often leaving the plant no means of propagation. Then also the increase in the area of cultivated land with advancing civilization is destroying the forests, so that year by year the extent of wild growth decreases. aasciupfiou  og flaht aijd hoots Golden Seal is a perennial, the plant reaching maturity, as far as harvesting for medicinal purposes is concerned, in four or five years. It has an erect, hairy stem and too leaves, palmately lobedf which are from tea to twelve inches in diameter. The rhizome and rootlets, which are the parts used in medicinal preparations, are bright yellow thoughout when fresh, and contain a quantity of yellow jul^ e, which gives off a rank, nauseating odor. The rhizome is from one-third to one-quarter of an inch thick, and from one to two feet long, with fibrous yellow roots produced from the sides, "hen dry the rhizome appears knotty and wrinkled; of a dull brown color outside, and pale yellow inside. It has a characteristic, quinine-life odor, and bitter taste. Each plant produces one white flower. The seeds, which are green at first, turn to scarlet in July, when they should be harvested, and dark brown in the early part of August, if left on the plant. SB0v.'Bi3-  oomiTtcms It is a recognizor fact that Hydrastis cannot !>© grown success Hilly in an open field, and that it is necessary to imitate natural conditions as ranch as possible. Sto different methods of accomp-lishing this result present themselves* the first to grow the herb in a plantation of trees, thus obtaining conditions identical with those under which it is found in nature, and the other to provide artificial shade» Both these methods have their disadvantages, the tress in the feraser depriving the plant of much of the nourishment and moisture in the soil, while the latter method is naturally the most ©aspens ive» Bie plants at the Botanical Gardens were grown under artificial shade* Ordinary laths, about two inches wide, were used to make the shelter, supported by posts driven into the ground at inter-vals of eight feet« she shed was about eight feet in height, completely ' covered with laths» In one-half the frame the laths were their own width apart, thus providing fifty per cent shade; in the other half they were placed closer together, in order to afford seventy-five per cent shade» She latter is considered to best represent the conditions in a forest of average density, and the plants were foimd to need this amount of pro-tection* In order to secure good drainage, beds were mde raised about four inches above the level of the ground, the edges being supported by boards» A li^ it rather sandy soil was used, into which a quantity of leaf mold had been introduced in order to secure the fertility and moisture retaining properties of forest soil» She plants were grown from seed, and allowed five years to reach raaturity» Each fall they were covered up with leaf mold to protect the roots in the winter» She plant will also propagete itself by the formation of new plants on the long slim fibre roots. After the first year, yellow buds will appear on the rhAzoroe, which, if not disturbed, will send up new stalks* If the rhizomes are taken up and divided, a bad being left on each piece, and planted at a distance from each other, separate plants will appear, which will reach maturity in four years, She bed in which the Hydrastis was grown measured 216 sq* ft, She plants were placed about I ft, apart. From this area, 60 lb., green weight, was obtained» Ihis would indicate that an aese grown under like conditions should yield about 10,000 lbf green weight, which is equivalent to 3,500 lb* dry weight* !IMs compares favorably with yields obtained in other localities, 1^ 3753 Til-IG MID  JggMk As the drug content of the roots varies considerably with the season, the crop should be lifted la either September or October when the leaves are dying down. Spring dug roots do not contain as ranch alkaloid, mid so command a lower price, than fall dag roots* She roots sent in for analysis were lifted on September 25, 1925* All the loose soil was shaken out, they were well washed, the moisture allowed to drain off, and then weighed (green weight). 2hey were then spread in thin layers on wooden desks covered with paper, which served to catch any fibrous roots becoming detached from rootstook* She drying was facilitated by turning the roots over each day. Shis was necessary on account of the tendency of the roots to mold if the drying is too slow* ae roots were reweighed at intervals, until after two weeks no further loss of weight was noticed, and they were assumed to be completely airdried* It was found that the roots lost approximately 655$ of their weight in drying* DI30US3I0IT OP AffAEfSIS Hydrastis canadensis is valued on account of the alkaloids which the roota contain» Of these the two most important are Hydras tine and Berberine. She roots also contain small quantities of canadine, meconln, and phytostearin» It is possible to separate the berberine and hydrastine owing to their different solubilities in ether, and to determine each separately» After having been thoroughly air-dried, the root was ground tip la an iron mortar until the powder would pass through an eighty mesh solve. It was then well mixed, and a sample taken by the method of quartering» Shis sample was then desiccated to constant weight, when it was ready for analysis* 3ie ground root was kept in a glass container, tightly stoppered, and protected from the light. Two different methods of analysis were used, that of the United States rharmacopoeia, ninth revision, which is the official method for obtaining the total alkaloids, and a method of Gordin I and Prescott , for the separation and determination of the hydrastine and berberine» u» s, p. imam d Introduce 10 g» of the powfered root into a 250 c.c* flask, and add 100 c.e. ether» Stopper, shake well, and allow to stand for 10 min*, then add 5 c.c» of 3H4GH water, and shake the flask vigorously every 10 min* for Z hrs. low add 15 c.c. distilled water, again shake the flask well, and when the drag has settled decant 100 o»c. soln», representing 5 grams of the root. Filter the sola» through a pledget of 1 Arch» Biarm., 1899, 23?, 441» purified cotton into a separator, and rinse the graduate and cotton with a little ether* Completely extract the alkaloids from the sola, by shaking out repeatedly with weak HgSO^ . Collect acid washings in a separator, add Sf%OH water untdl decidedly alkaline, and completely extract alkaloids bynshaking out repeatedly with ether* Svaporat© combined ether washings to dryness, dry the .residue to constant weight at 100°, and weigh, weight is amt* of ether soluble alkaloids from 5 g. Hydrastis* HBTH033 OF S0B3HT AHD PRB3COTT Stir 10 g* of the powdered root into a paste with a few c*c* of a mixture of alcohol, concentrated W3UM,  and ether, in the proportions Is 1:6, and kept in a stoppered jar for several hours, (overni^ it)• Dry the mixture in a current of air, until all the UH OH lias volatilised, and afterwards in a vacuum over EgSG^  for 5 - 6 hrs* Transfer the dry substance to a Soxhlet extractor, the jar being rinsed with powdered BaCSO^ Jg, and extracted with 40 - 50 c.c* absolute ether until the residue from the evaporation of a few drops of the extract gives no reaction with :layer's or Wagner's reagents* The ethereal extract contains the hydratslne* Pass a current of air through the Soxhlet tube until all the ether has evaporated, and extract the residue with 40 - 50 c.c. of alcohol. Continue extraction wit11 the extract is colorless, ash the alcohol extract Into an evaporating dish together with a little hot water and dilute BAO, and evaporate with the addition of water until all the alcohol is expelled. Add a further small quantity of dilute HAc, cool liquid, filter into a 300 - 400 c*o* Srlenmeyer flask and wash the filter. Treat filtrate with 6 - 8 c.c. of acetone, and a 10$ soln. of ITaOH drop by drop until the precipitate formed no longer disappears upon shaking, and the liquid acquires a strongly alkaline reaction. Stopper flask, shake in a circular direction for 10-15 min., and then allow to stand for 2 - 3 hrs. Wash crystals of berberine-acetone formed until the washings are colorless, return to flask, and treat with 4 c.c, of 1^ 30^  and water tip to 100 - 200 c.c. Heat flask in hot water until ppt. dissolve^  transfer to long-necked flask, and boil gently fofc 2 hrs., adding water when necessary. Cool liquid, mix with 100 c.c. of H/20 K1 soln., dilute mixture to I litre, and allow to stand overnight. Filter liquid, mix 500 c.c. of the filtrate with 50 c.c. of u/20 Agt?0_ soln., add a little HHO , o o and dilute to I litre. Shake, filter, and titrate 500 c.c. of the filtrate with Ij/40 IIH^ CIFSt using ferric alum as indicator. Twice the number of c.c. of UH CHS used is equal to the number of c.c. of If/20 EI soln. consumed 4 by the berberine in 10 g. of root, The number of c.c. of H/20 K1 soln. consumed multiplied by 0.167125 gives the percentage of anhydrous ber-berine, RESULTS OF AIIALYSR3 The U.S.P. standard for the "ether soluble" alkaloids in Hydrastis is not less than 2*5$, CWing to the fact that water is present, much of the berberine as well as the hydrastine must also be present in the final residue. Great difficulty was encountered in the prooedure owing to the presence of oil and resinous matter which was extracted with the alkaloid. This difficulty was finally overcome by first treating the powdered root with petroleum ether, in which both hydrastine and berberine are insoluble.This removed the fixed oil. 3y this method of analysis the drug showed an alkaloidal content of 2.8'^ , which is above the Pharmacopoeia standard. By Grordln and Prescott*s method, the drug was found to contain 1.5^  hydrastine and I.fy berberine, which gives a total alkaloidal content of 3*2$* me difference in the two results is doubt-lessly due to the fact that all the berberine is not extracted in the Ü* 3* process. ühese results compare favorably with other reports on the alkaloidal content of various crops. Hordingv 2 who has grown hydras tis for many years, gives the average amount of alkaloid as 2,6 to 2*7^ » Perrin,3 who analysed both the wild and cultivated root, gives 2,5$ as the average amount in the former, and 3*1 $ in the latter* C017CTJ33 IOIf 3b» price paid at the present time for Hydrastis offers a great inducement to anyone contemplating growing it en a com-mercial scale. SSie highest current price for fall dog roots is §I6*75*lb,, this being for the dried root* She price of this herb fluctuated a good deal some years ago, owing to the alternate oversupply and scarcity, but this was when a good supply was at hand in the forests. But a fairly steady advance in price over the last twenty years can be noted. In 1904 the demand for the root was increasing, and the price rose to #1,40 a lb., the first timo that a noticeably high. price was paid. In 19II the price rose to $4,50 a lb. 3ie price Increased yearly until 1920, when it readied #12,00 a lb* In the last six years the price has risen from 512,00 to $16.00, which gives a fair idea of the stability of the market, Calculating on the basis of 3,500 lb* to the acre, the gross returns from an acre of Hydrastis should bo somewhere in the neighborhood of #60, 000* 2 , Ginseng said Other Medicinal Plants. 3 Usar* J. Iharm*, 1904, 153, 226 - 10 -ASHOM BELLASOHHA IUgROmJOTIOlT Atropa belladonna, or deadly nightshade, has been known from early times on account of its poisonous properties, and its mydriatic action upon the eyes. The name, {from the Italian «beautiful lady" I, is said to be derived from the fact that the berries were used as a cosmetic. Preparations from the leaves, roots, and stems have a wide application in pharmacology both for their mydriatic property, and their paralytic effect on the muscles and the central nervous system. PSSGRIPglOiT OF PIAI1S Belladonna is a perennial belonging to the natural order Solanaceae. It is a native of the region from southert Europe to India, but is widely naturalized in most civilised countries. It is a beautiful, spreading plant, sometimes attaining the height of six feet. She leaves are entire and ovate, and dark green la color. Purple flowers occur either singly or in pairs, and later black, shiny berries, about the size of a currant, appear. She whole plant has a dis-agreeable, bitter odor. She rootstock is long, thick and fleshy, with small rootlets at the sides and end. SBOfflHS COirPISIOIIS One plant of Atropa belladonna was grosa at the Botanical Gardens. Shis was grown from seed, which was planted in light, rather sandy soil» on June 20, 1923. She herb was grown in an open, sunny place, on land which had a gentle slope to ensure good drainage. At the time of lifting, October 6, 1925, the plant was five feet tall, - -covering an area of about 4 sq. ft* She yield obtained from this specimen was I lb* of leaves and 10 lb. of root, green weight* In order for the plants to attain full growth it would be necessary to allow 2 sq» yd. to a plant* Shis would give a yield of 2400 lb, of leaves and 24,000 lb, of root to the acre, or 1200 lb* of leaves, and 10,800 lb» of root dry wt» Upon drying {under the same conditions as the Hydrastis), the leaves lose 50£, the roots 55$, of their moisture. DISCPSSIOff OF A1IALYS1S The chief alkaloids of Atropa belladonna are hyoscyamlne and atropine, although a small quantity of hyo;;aine la also present» Wo  reliable methods have been worked out for the reparation of these two alkaloids, as the hyoscyamine is readily converted into at-ropine in the process of extraction* Therefore tho only determination made is that of total alkaloid. The leaves and root were each ground in an iron mortar until they would pass through an eighty mesh sieve $ desiccated to constant weight, and were then ready for analysis. The roots and leaves were kept in a closed container away from the li^ it. aie method of the United States Pharmacopoeia was used for the analysis of both leaves and roots. Then other samples were treated using a sli^ it modification of this procedure, in that a Soxhlet apparatus was used for the extraction, and the final residue was first weighed as a check before titrating. U, S. P. IBTHOD Roots» Introduce 15 g. of powdered root into a 250 c.c. flask, and add 150 o.o* of a mixture of dOlg, I vol», and Bto0, 2 vol. Stopper, shake well, and alio.? to stand for 10 min», then add 5 c.c. of IJH^ OH water and shake the flask vigorously every 10 min. for two hrs. How add 15 c.g. of distilled water, again shake the flask well, and when the drug has settled decant 100 c.c. of the sola, representing 10 g. root. Filter soln. through a pledget of purified cotton into a separator, and completely extract the alkaloids from the sola, by shaking out repeatedly with weak %SQt* Collect acid washings in a separator, add TU^ OH water uatftl decidedly alkaline, and cospletely extract the alkaloids by shaking out repeatedly with GHClg» Evaporate combined CHClg washings to dryness, dissolve the alkaloids from the residua in exactly 5 c.c* of Il/lO %S04, and titrate the excess of acid with S/50  13)11, using cochineal as indicator Every c.c. of Ii/10 I^ SO^  consrssod corresponds to 28.92 milligrams of the total alkaloids. Leaves« Proceed as above, using 15 g. of leaves, and increasing the amount of water addad after maceration to 25 c.c., and, before titration, treating the final residue twice with 5 c.c. of BtgO, evaporating to dryness each time. RESULTS AITS OOirCIffSlOU % the U.S.P. method, the amounts of total al-kaloids found were: leaves, .55^ , roots .64^ , Sy using the Soxhlet ex-tractor, more of the alkaloid was removed, giving the result of loaves, .60^ , roots ,&Tpm She U.S.P. standard for alkaloidal content is leaves, not less than and roots not less than .45p. other reports have shown that there is a great variation in the amounts of alkaloid present, some investigators reporting more in the cultivated plant, others claiming to have found more in the wild. It is a recognised fact that second year plants contain more alkaloid than those only one year old. — X3 — POBOMTLM PBLTATER1. IITTROIXJC TIOII« Podophyllum pel ta turn, or SSay apple, Is another •useful drug plant» Other cannon names by which it Is known in various localities are mandrake, wild lemon, hog apple, devil's apple, and vege-table calomel» Although not of such Importance as Golden Seal, it has a fairly wide application as a catharic. Of later years the use has diminished somewhat doe to certain objectionable properties which it possesses, such as the poisonous effect of largo doses» 3ES03IPTI0N OF PLAITT» The plant is an indigenous perennial, belonging to the barberry family {Berberidaca©)» It is found from western Quebec to Minnesota, and south to Florida and Texas, growing in low woods, usually in dense patches • It is of erect growth, from one-half to two feet In height, with smooth, dark green foliage, and Is a conspicuous plant throughout its range in early spring* She leaves are two in number, and about one foot in diameter* A wasy White, solitary flower appears in May, and later a lemon-shaped fruit, which is first green, and turns yellow upon ripening» This fruit is edible, and lias a sweet, honey-like flavor. The root Is the Important part of the plant for medicinal purposes* She horizontally creeping roots took is from one to five feet in length. It is flexible, smooth and round; dark brown on the outside and whitish or fleshy within* At intervals of a few inches are thickened joints, with tufts of roots on the lower side» 14 -ffRowiM mmœiom as Podophyllten was grown under identical conditions te the Golden Seal. It was grown front seed, and -was five years old at the time of lifting, Oct* 20, 1925» From an area of 80 sq, ft*, a yield of IS lb, of root (green weight ) was produced, which would indicate a yield of approximately 8000 lb* to the acre, or about 3200 lb. dry wei#it, She root lost about &>Ja of its weight upon drying, which was done in the same way as the Hydrastis, DIS0ÏÏSS I01I OF AmiYOXS She Podophyllum root contains a resin which is used in medicinal preparations* She amount of resin present was determined by the United States pharmacopoeia method* The root was ground up into a fine powder{until it would pass through an eighty mesh sieve), and then desiccated to constant wei^it. ÏÏ.3.P. METHOD Moisten 10 g* of powder with 500 c.c* of StOH, and pack: in a cylindrical percolator; then add enough StOH to saturate the powder and leave a stratum above it. Then liquid begins to drop from the percolator, close lower orifice, closely cover percolator, and macerate for 48 hrs. Then allow percolation to proceed, gradually adding StOH until percolate ceases to produce more than a slight turbidity when introduced into water. Distil off the StOH until the percolate is reduced to the consistency of thin syrup, then pour slowly, with constant stirring Into I litre of water, mixed with 10 c.c. of HC1 and cooled below 10° C. •hen ppt* has settled decant liquid, and wa3h ppt* twice with water by décantation* Spread resin in a thin layer upon a strainer, and dry by «• is exposure to the air*, la a cool place, protected from the light, RESULTS The root investigated was found to contain 3.1 % of resin. She Pharmacopoeia standard for Podophyllum is given as not less than 3.0 % resin, POHJLPS THI CHOC ARPA, _INTMJCT20IT_ Balm of Gilead, or Ilecca balsam, an exudation from the Balsamodendron gileadense, lias been of common use in pharmacology for some years as a stimulant to the mucous membranes In chronic bronchitis and catarrh. As a substitute for this the balsam from the buds of the Populus trlchocarpa is used. This balsam is considered if anything superior to the original Balm of (Jilead, by which name it is sometimos erroneously called. g-BQOTS OOTTDITIOffS The Populus trlchocarpa Is a tall, branching tree, and consequently it Is difficult to gather the buds. It was thought that It would be possible to grow cuttings whieh would not be allowed to attain a height which would make harvesting difficult. Accordingly cuttings wwe planted at intervals of about three feet. The buds, which contain the balsam, were collected in the latter part of January from one year old cuttings. As the crop was In no-.my representItive of the acreage, owing to the youth of the trees, no calculations were as to the yields possible per acre. - 16 -33TgRMDTA?I0H OF BAISAM IXT BUDS Ylien the buds were recieved, they were weighed and placed la Q Soxhlet extractor, and the balsam completely extracted with ether* The ether was then distilled off, leaving a thick brown vis-cons mass, of highly agreeable aromatic odor* The yield of balsam from the buds was 31 %m It was completely soluble in ether and alcohol, partly soluble in chloroform, and insoluble In water* DBT5RMIIIATIOU OF SPECIFIC GRAVITY The density of the balsam was obtained through the method of mixtures. A 10 c*c. specific gravity bottle was cleaned, dried and weighed; then filled with distilled water at 20° <5., and again weighed* The bottle was then emptied, dried, and about 2 e.c* of the balsam introduced by means of a glass rod* It was then weighed. Distilled water was poured on the balsam until the bottle was completely filled, and the bottle and contents weighed at 20° C. From this determination the density of the balsam was found to be 1*134 at 20° C. SAPONIFICATION VAEPE The saponification value was found as follows: I g, of balsam was dissolved in 10 c*c* of alcohol and treated with 25 c. c* of IT/2 alcoholic M)H, The soln was than boiled on a steam bath under a reflux for one hour, when it was diluted with alcohol, and titrated with u/io HgSQ^ , Using this method the average of several closely agreeing values was 170*8* ACID álTD BSTSR VALUES The acid value was found by direct titration*, 2 c* c* of the balsam being dissolved In alcohol, and titrated with u/l0 H2S0A She acid value was found to be 88.0« Th® ester value is given by the differ ence between the saponification and acid values, and is accordingly 82.0. conclusion The different constants of this balsam differ greatly from those of the Mecca balsam. She substance also contains a quantity of volatile oil, which has not yet been investigated. xssgmaMiQ'r OF msm in mim* .alcohol« AH3 wATgj aimEESS. 9xe purpose of this investigation was to find, a practical method of analysis for the ether, alcohol and water mistares resulting from the catalytic dehydration of alcohol to ether by means of Al^ Og* In maay of these mixtures the yield of ether is sufficiently high to cause a separation into two layers-» It was necessary to tafee this fact into con-sideration in the choice of a method of analysis * A chemical method, that of oxidation by means of E Mb in an acid solution, was first tried.» It was thought that it would be possible to oxidise all the- alcohol to acetic acid without aa appreciable amount of the ether being, touched,'and thereby changing the conditions in a second sample, to oxidise both the ether and the alcohol» The ether .could then be obtained by difference» Shis was found to be unsatisfactory in practice, owing to the varied amount of ether attacked in the alcohol oxidation, and the tendency of the Ha 02 to precipitate out» A similiar method, using i^ Or^ O^  as oxidising, ageht, had. already been tried out In this laboratory with unsatisfactory results» Tfee following method of salting out the ether from the mixture with saturated 3a CI solution was then tried, giving results ac-curate to within 1,0» Wolff, Ohem. ;;tg, M , 1193, uses the principle of salting out for the determination of ether and benzene in alcohol. An adaptation of Wolff*» method lias- also been used by H» II* Pease and CSii Chao Tung, 3, "jaer. Ohem. So©*, Vol. 46, 1924 , 2397, for their analysis in the production of ether from alcohol» xhe latter authors found that upon shaking a mixture of ether and alcohol with about four times its volume of salt solution, the greater part of the ether separated out* They show a curvo giving the correction, the trae volume of the ether being plotted against the volume separating* Shis correction Is necessary owing to the solubility of the ether in the alcohol and water of the lower layer , and also the solubility of the alcohol in the ether of the upper layer* '"he solubility of the water la the ether is negligible *A shrinkage in volume also takes place owing to the fact that the Initial volume of ether is measured at room o temperature, and that of the upper layer at 10 C* The mixtures of ether, alcohol and water used were those which would be produced in the different percentage conversions of 50 g* of alcohol to ether* Their carve shows that when the yield of ether is low, the separation is greater than the true volume, and that when the yield is high, the separation is less than the true volume* Preliminary trials were made with corresponding mixtures, in order to see whether these values for the correction could be duplicated. The values obtained coincided with those gisten for the hi jher yields, but not for the lower, the volume separating being always less than the true volume* Accordingly it was thought necessary to carry out a series of determinations, in order that this method could be used for the mixtures under consideration. It was found convenient to work with a smaller volume, 20 c.c* being chosen as the most suitable* For this purpose a 50 c.c* burette, sealed to the neck of a 100 c*c. volumetric flask, was used. Amounts of ether and alcohol in the required proportions were run into the flask from two burettes, the room temperature being noted* The flask was then corked, and suspended in a water bath at 10° C* After the addition of IOOc.c. of saturated salt solution, the flask was tl^ itly corked, and vigorously shaken for a few minutes. Ehis vigorous shaking was continued until complete equilibrium between the two layers had been reached* The 0 volmm of the upper layer was then read at 10 0* She first mixtures used were of ether and alcohol alone, having a total volume of 20 c.c., and varying in composition from 100$ ether to XOOfj alcohol. SABLE I Measured Vol. Measured ?ol* Bther Alcohol Separation Correction 20*0 C.C* 0 c.c* 18,6 c.c. 1.4 18.I C.C. 1*9 c.c. 16*8 c.c* 1.3 14.8 c.c. 5*2 c*c* 13*6 c.c. 1*2 12,0 C.C. 8,0 c*c* 10.9 c.c. 1*1 c.c. 10.I c.c. 8*9 c.c. 1*0 7*6 c.c* 12.4 c.c. 6*7 c.c. 0*9 5*8 c.c* 14*2 c.c. 4.9 c.c. Q.9 4*2 c.c* 15.8 c.c. 2*9 c.c. 1*3 2*9 c .c * 17*1 c.c. 0.7 c.c. 2*2 Beyond this point no appreciable separation occurred, the small volume of ether all dissolving in the bottom layer. It will be seen from this table that when the alcohol is more than three times the volume of the ether, the results become inaccurate. 50 show the effect of small quantities of water on the separation, this determination was repeated, using the same amounts of ether and alcohol, but adding water in amounts varying from I to 5 c.c* An excess of solid salt was present. She addition of water had no * apparent effect on the separation, the values obtained coinciding with those of Table 1 «dSorefore if 20 c.c» of an other, aloohol and water mix tare are taken for analysis, the only influence of the water would be to make the volume of ether and alcohol smaller in comparision to the amount of salt solution« in the case cited above, the water was added in addition to the 20 c.c. of ether and alcohol, and so the proportion of salt solution to ether and alcohol remained unchanged. To deter nine what difference the amount of salt solution present makes in the separation, the volume of the mixture was varied, that of the salt solution being kept constant« With pure ether, as might be expected, a constant amount dissolves in the salt solution, independent of the total amount of ether present.This is due to the slight solubility of water in ether, '.hen alcohol is present, however, the correction varies with the total volume of the mixture, the correction becoming greater as the volume decreases .This is in accordance with the partition law. The concentration of the alcohol in the water layer is diminished,and therefore it must also become less in the ether layer, causing a shrinkage in volume. Two different mixtures were tried, the proportion of ether to alcohol being kept constant while the total volume of the mixture varied from 20 to 5 c.o.; 100 c.c. of salt solution being used in each case. TA3LB II Total Vol. lleasured Vol. Measured Vol. Separation Correction Mixture Ether Aloohol 20 c.c. 14«8 c.c. 5.2 c.c. 13.6 c.c. 1,2 15 c.c. II,I c.c. 3.9 .c.c 9.85 c.c. I. 25 10 c.c. 7.4 c.c. 2.6 c.c. 6.1 c.c. 1.3 5 c.c. 3.7 c.c. 1.3 c.c. 2,35 c.c. 1,35 fötal Vol % Mixturo 20 o*o. 15 c*c* IO o«o. 5 c*e* would occur if 5 c.c. of water were present in 20 c.c. of the mixture â&e only .05 c.c. in the first case , and .15 c.c* in the second case, and the change in the ratio of alcohol to ether is considerable in the two tables. In the dehydration of alcohol, when the percentage conversion of alcohol to ether is small,the water produced will not reach© the amount of 2 c.c. for an original volume of 20c.c. alcohol. In the higher ' percentage yields, the water equivalent to this amount of alcohol will not be over 3 o.e., and as is seen above, the change in correction lies within the experimental error. In the caso of some of the alcohol being dehydrated to form ethylene, an excess of -water would be present. Under properly regulated conditions, however, the conversion to. methylene will never be more than for which the amount of water foraed is under I e.G. It was found that the presence of ethylene dissolved in the mixture did not affect the separation in any way. Mixtures of ether, alcohol and water were now made up identical with those formed in the different percentage conversions of alcohol to ether by weight. Using the equation 2 EtOH ÊT^O •mm. § o* mBL3 III Measured Vol* Measured Toi* Separation Correction Ether Alcohol 9*9 c.c* 10,1 c.c* 8*9 c*c* 1*9 7*4 0*0. 7.6 c.c* 6*25 c.c. I.15 4*95 C.O. 5.05 c.c. 3.65 C.C. 1.3 2.5 c*c* 2*5 c.c. I.I c.c. 1,4 From this it is seen that changes in the correction which and taking the density of ether as #719 and that of alcohol as .793 at 15° C»* the volumes corresponding to the different percentage conversions of alcohol to ether can he calculated« As has been previously stated, 20 c.c. of alcohol was found to be a convenient Initial volume for the catalytic process,, and the calculations were made from this* She fol-lowing table gives the observed separation for the mixtures of compos it ion Indicated. She column headed percentage conversion shows the percentage of the total alcohol equivalent of the mixture which is equal to the ether present on the basis of the above equation* In practice a little more than 20 c.c* of alcohol was run over, in order that the conversion to ethylene might be taken Into account* Therefore in practice the percentage conver»-sions to ether cannot be taken directly from the table* These values served merely as a basis for the calculations. Owing to the shrinkage in volume upon mixing, the volume of the mixture resulting from an initial volume of 20 c.c. of alcohol is very nearly 20 c.c. TABLE IV % Measured Measured Measured Separation Correction Conversion Vol. Ether Vol. Ale. Vol* vater 100$ 17*8 c.c* 0 c.c. 3*1 C.C* 16.4 c.c. 1*4 90% 16*3 c.c* 2*0 c.c. 2*8 c.c. 15*05 c*c. 1.25 80*5 14*3 c.c* 4*0 C»C* 2*5 c.c. 13,1 c.c. 1.2 7<$ 12*6 c.c. •5*7 c.c* 2*2 c.c. 11*7 c*c. I.I . 60$ 10*7 c.c* 8.0 C.C. 1*9 c.c. 9*6 c.c. I.I 50% 8*9 c.c. 10*0 C.C. 1*6 o*c. 7*9 c.c. 1.0 40/3 6.9 c.c* 12*0 c*c* 1*2 c.c* 6*0 c.c. 0.9 30$ p.I5 c.c. 13*8 c.c* 0*9 c.c. 4*2 c.c. 0.9 20/5 3*3 c.c. 15*8 c.c. 0*6 c.c. 1*2 c.c. 2*1 As before, the values became Inaccurate in mixtures re-presenting conversions below 30% owing to the large amount of alcohol present* The corrections show no appreciable change from those of Table I, These values are for ether and alcohol in a definite series of ratios* In order to find how the correction would vary with different ratios of alcohol to ether, a fixed amount of ether was taken with varying amounts of alcohol, giving the following results* TABLE V* Measured Vol* Measured Vol* Separation Correction Ether Alcohol 14*0 c.c* 0 c.c* 12.6 c»c* 1.4 14*0 c.c. 2*0 c.c. 12*65 c.c. 1*35 14*0 c.c. 4*0 c.c. 12.75 c.c. 1*25 14.0 c.c* 6*0 c.c, 12*8 c.c. 1*2 TABLE VI 10*0 c.c. 0 c.c. 8.6 c.c. 1.4 10.0 c.c. 2.0 c.c. 8.7 c.c. 1.3 10*0 c.c. 4.0 c.c* 8*8 CoC. 1,2 10,0 c.c* 6,0 c.c. 8,9 c.c* I.I 10,0 c.c. 8*0 c.c, 9*0 c.c. 1,0 10,0 c.c. 10*0 C.C. 9*05 c.c. .95 It would appear from these values that there is a rela-tively large increase in the correction as the amount of alcohol decreases. However, for a decrease of 6 c.c. In the alcohol volume from that amount which would bring the total volume of the ether and alcohol mixture up to 20 c.c., the error Introduced by using the values for the corrections - G -given in Table I is under 2JS in both cases» Therefore for «light changes in the ratio of ether to alcohol the change in correction is small» For the analysis of mixtures resulting from the conversion of alcohol to ether, this method is, therefore, accurate to within which is the limit of accuracy of the burette readings. I,hen the ether has been determined in this manner, approximate values for the alcohol and water could be obtained fro© specific gravity measurements. If it should be more convenient to use larger volumes, the volumes of the mixture, salt solution, and correction, can be increased in the same ratio. For mixtures in which the ratio of the ether to the alcohol and water is not known, this method serves as an approximate determination, provided that the amount of ether is not less than one-third, and the quantity of water not more than one-half, the total volume of the mixture. Under these conditions the error Is within BIBLIOGRAPHY Gordia, R. and ïrescott, T, Archives of Pharmacology, 1894 Volume S3?, page 441 Harding, A* H. "Ginseng and Other Medicinal Plante"« Published Et« louis, Ko., 1906 Henry, S.A. "'Eie Vegetable Alkaloids" Allens Comercial Organic Analysis, Vol. VI Pease, H.ÏT. and Yung, 0.0« Journal of the American Chemical Society, Vol. 46, 1924, page 2397 BarrIns, IT« American Jounal Of Pharmacology # 1399 Vol. 253, page 642« Pharmacopoeia of the United States, ITlnth Tievision. 

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