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Ma, Tianxiao

University of British Columbia

DNA electrochemical (E-DNA) biosensor has developed rapidly and gained worldwide attention from many fields such as medical science, food industry, and environmental analysis. However, current electrochemical DNA biosensors have a few critical limitations that prevent E-DNA sensors from becoming more valuable and useful for commercial applications. This thesis addresses some of the issues of E-DNA biosensors such as thermal stability and functionality using electrochemistry and in-situ fluorescence microscopy. The work in this thesis used single crystal gold bead electrode as the substrate which provided a great opportunity to build the near ideal DNA SAMs with far less defects than on the polycrystalline gold which is typically used in the sensor fabrication. Together with the single crystal Au bead as the substrate and in-situ fluorescence microscopy, a thorough investigation on the properties of DNA SAMs prepare with and without potential control (Edep vs. OCPdep) was conducted. The results demonstrated Edep with superior thermal stability in solution and higher sensitivity than OCPdep. A detailed analysis on the influence of surface crystallography on the thermal stability and the packing behavior of both types of DNA SAMs prepared by Edep and OCPdep was also provided.

Text

2022-12-21

Attribution-NonCommercial-NoDerivatives 4.0 International

http://hdl.handle.net/2429/83457

Chemistry

University of British Columbia

UBCV

http://creativecommons.org/licenses/by-nc-nd/4.0/