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A genetic barcoding system to isolate target clones based on single-cell transcriptomic profiles Sakata, Rina
Abstract
Single-cell transcriptome sequencing and cell lineage tracing technologies have enabled high- resolution mapping of different cell states and trajectories during stem cell maintenance and differentiation. Nevertheless, using such technologies, we are not able to retrieve living clones of the studied cells for further molecular or functional analysis. In this thesis, I describe the development of a technology, scCloneSelect, that enables sorting clones of a target cell state identified by single-cell RNA sequencing through combining cellular DNA barcoding and CRISPR base editing technology. Furthermore, I explore the application of scCloneSelect to study factors underlying different cell fates during stem cell differentiation, reprogramming and maintenance.
Item Metadata
| Title |
A genetic barcoding system to isolate target clones based on single-cell transcriptomic profiles
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| Creator | |
| Supervisor | |
| Publisher |
University of British Columbia
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| Date Issued |
2022
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| Description |
Single-cell transcriptome sequencing and cell lineage tracing technologies have enabled high- resolution mapping of different cell states and trajectories during stem cell maintenance and differentiation. Nevertheless, using such technologies, we are not able to retrieve living clones of the studied cells for further molecular or functional analysis. In this thesis, I describe the development of a technology, scCloneSelect, that enables sorting clones of a target cell state identified by single-cell RNA sequencing through combining cellular DNA barcoding and CRISPR base editing technology. Furthermore, I explore the application of scCloneSelect to study factors underlying different cell fates during stem cell differentiation, reprogramming and maintenance.
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| Genre | |
| Type | |
| Language |
eng
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| Date Available |
2022-09-01
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| Provider |
Vancouver : University of British Columbia Library
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| Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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| DOI |
10.14288/1.0418590
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| URI | |
| Degree | |
| Program | |
| Affiliation | |
| Degree Grantor |
University of British Columbia
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| Graduation Date |
2022-11
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| Campus | |
| Scholarly Level |
Graduate
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| Rights URI | |
| Aggregated Source Repository |
DSpace
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Rights
Attribution-NonCommercial-NoDerivatives 4.0 International